(+)-abscisic acid 8'-hydroxylase
In enzymology, a (+)-abscisic acid 8'-hydroxylase (EC 1.14.13.93) is an enzyme that catalyzes the chemical reaction
- (+)-abscisate + NADPH + H+ + O2 8'-hydroxyabscisate + NADP+ + H2O
| (+)-abscisic acid 8'-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.93 | ||||||||
| CAS number | 153190-37-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The four substrates of this enzyme are (+)-abscisate, NADPH, H+, and O2, whereas its three products are 8'-hydroxy-abscisate, NADP+, and H2O.
This enzyme belongs to the family of oxidoreductases, to be specific those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor. This enzyme participates in carotenoid biosynthesis.
Nomenclature
The systematic name of this enzyme class is abscisate,NADPH:oxygen oxidoreductase (8'-hydroxylating). Other names in common use include
- (+)-ABA 8'-hydroxylase and
- ABA 8'-hydroxylase.
References
- Cutler AJ, Squires TM, Loewen MK, Balsevich JJ (1997). "Induction of (+)-abscisic acid 8' hydroxylase by (+)-abscisic acid in cultured maize cells". J. Exp. Bot. 48 (10): 1787–1795. doi:10.1093/jxb/48.10.1787.
- Krochko JE, Abrams GD, Loewen MK, Abrams SR, Cutler AJ (1998). "(+)-Abscisic acid 8'-hydroxylase is a cytochrome P450 monooxygenase". Plant Physiol. 118 (3): 849–60. doi:10.1104/pp.118.3.849. PMC 34795. PMID 9808729.