Bicinchoninic acid

Bicinchoninic acid
Names
	Preferred IUPAC name [2,2'-Biquinoline]-4,4'-dicarboxylic acid
	Other names Bicinchoninic acid; 4,4'-Dicarboxy-2,2'-biquinoline
Identifiers
CAS Number	1245-13-2 ;
3D model (JSmol)	Interactive image;
ChEMBL	ChEMBL431482 ;
ChemSpider	64223 ;
ECHA InfoCard	100.013.628
PubChem CID	71068;
UNII	CX56TX9Y1I ;
CompTox Dashboard (EPA)	DTXSID8061638 ;
	InChI InChI=1S/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26) Key: AFYNADDZULBEJA-UHFFFAOYSA-N ; InChI=1/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26) Key: AFYNADDZULBEJA-UHFFFAOYAF;
	SMILES O=C(O)c1cc(nc2c1cccc2)c3nc4ccccc4c(c3)C(=O)O;
Properties
Chemical formula	C20H12N2O4
Molar mass	344.326 g·mol−1
Appearance	Cream colored powder
Odor	Characteristic odor
Melting point	365 to 367 °C (689 to 693 °F; 638 to 640 K)
Solubility in water	Partially soluble in cold water, hot water
Hazards
Main hazards	Will irritate eyes and mucous membranes.
Safety data sheet	External MSDS
NFPA 704 (fire diamond)	1
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

Bicinchoninic acid /baɪsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu⁺ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[1]

References

Pierce BCA Protein Assay Kit

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[1] Pierce BCA Protein Assay Kit