Cfr10I/Bse634I

In molecular biology, the Cfr10I/Bse634I family of restriction endonucleases includes the type II restriction endonucleases Cfr10I and Bse634I. They exhibit a conserved tetrameric architecture that is of functional importance, wherein two dimers are arranged, back-to-back, with their putative DNA-binding clefts facing opposite directions. These clefts are formed between two monomers that interact, mainly via hydrophobic interactions supported by a few hydrogen bonds, to form a U-shaped dimer. Each monomer is folded to form a compact alpha-beta structure, whose core is made up of a five-stranded mixed beta-sheet. The monomer may be split into separate N-terminal and C-terminal subdomains at a hinge located in helix alpha3.[1] Both Cfr10I and Bse634I recognise the double-stranded sequence RCCGGY and cleave after the purine R.[2]

Recognition sequence     Cut
5' RCCGGY                5' ---R   CCGGY--- 3'
3' YGGCCR 	         3' ---YGGCC   R--- 5'
Bse634I
crystal structure of citrobacter freundii restriction endonuclease cfr10i at 2.15 angstroms resolution.
Identifiers
SymbolBse634I
PfamPF07832
InterProIPR012415

References
  1. Grazulis S, Deibert M, Rimseliene R, Skirgaila R, Sasnauskas G, Lagunavicius A, Repin V, Urbanke C, Huber R, Siksnys V (February 2002). "Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence". Nucleic Acids Res. 30 (4): 876–85. doi:10.1093/nar/30.4.876. PMC 100338. PMID 11842098.
  2. Bozic D, Grazulis S, Siksnys V, Huber R (January 1996). "Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution". J. Mol. Biol. 255 (1): 176–86. doi:10.1006/jmbi.1996.0015. PMID 8568865.
This article incorporates text from the public domain Pfam and InterPro: IPR012415
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.