Cystatin A

Cystatin-A is a protein that in humans is encoded by the CSTA gene.[5][6]

CSTA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1CYU, 1CYV, 1DVC, 1DVD, 1GD3, 1GD4, 1N9J, 1NB3, 1NB5, 3K9M, 3KFQ, 3KSE
Identifiers
Aliases	CSTA, AREI, STF1, STFA, Cystatin A, PSS4
External IDs	OMIM: 184600 MGI: 1924020 HomoloGene: 3819 GeneCards: CSTA
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3q21.1 Start 122,325,248 bp[1] End 122,341,969 bp[1]
Gene location (Mouse) Chr. Chromosome 16 (mouse)[2] Band 16|16 B3 Start 36,221,468 bp[2] End 36,257,444 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • peptidase inhibitor activity • protease binding • protein binding, bridging • endopeptidase inhibitor activity • structural molecule activity • cysteine-type endopeptidase inhibitor activity Cellular component • cytoplasm • intracellular • cornified envelope • extracellular • cell nucleus • cytosol Biological process • negative regulation of proteolysis • peptide cross-linking • negative regulation of peptidase activity • cell adhesion • keratinocyte differentiation • negative regulation of endopeptidase activity • cornification • cell-cell adhesion Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1475	76770
Ensembl	ENSG00000121552	ENSMUSG00000095620
UniProt	P01040	Q9D8D6
RefSeq (mRNA)	NM_005213	NM_029733 NM_001384090
RefSeq (protein)	NP_005204	NP_084009 NP_001371019
Location (UCSC)	Chr 3: 122.33 – 122.34 Mb	Chr 16: 36.22 – 36.26 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.[6]

Interactions

Cystatin A has been shown to interact with Cathepsin B[7][8] and CTSL1.[8][9]

See also

• Peptide Transporter Carbon Starvation (cstA) Family

References

1. GRCh38: Ensembl release 89: ENSG00000121552 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000095620 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hsieh WT, Barrick JL, Berrettini WH, Chan MM, Fong D (Jun 1991). "A PstI DNA polymorphism in the human stefin A gene (STF 1)". Nucleic Acids Res. 19 (7): 1722. doi:10.1093/nar/19.7.1722-a. PMC 333958. PMID 1674139.
6. "Entrez Gene: CSTA cystatin A (stefin A)".
7. Pavlova, Alona; Björk Ingemar (Sep 2003). "Grafting of features of cystatins C or B into the N-terminal region or second binding loop of cystatin A (stefin A) substantially enhances inhibition of cysteine proteinases". Biochemistry. United States. 42 (38): 11326–33. doi:10.1021/bi030119v. ISSN 0006-2960. PMID 14503883.
8. Estrada, S; Nycander M; Hill N J; Craven C J; Waltho J P; Björk I (May 1998). "The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L". Biochemistry. UNITED STATES. 37 (20): 7551–60. doi:10.1021/bi980026r. ISSN 0006-2960. PMID 9585570.
9. Majerle, Andreja; Jerala Roman (Sep 2003). "Protein inhibitors form complexes with procathepsin L and augment cleavage of the propeptide". Arch. Biochem. Biophys. United States. 417 (1): 53–8. doi:10.1016/S0003-9861(03)00319-9. ISSN 0003-9861. PMID 12921779.

Further reading

• Järvinen M, Rinne A, Hopsu-Havu VK (1988). "Human cystatins in normal and diseased tissues--a review". Acta Histochem. 82 (1): 5–18. doi:10.1016/s0065-1281(87)80043-0. PMID 3122506.
• Brown WM, Dziegielewska KM (1997). "Friends and relations of the cystatin superfamily--new members and their evolution". Protein Sci. 6 (1): 5–12. doi:10.1002/pro.5560060102. PMC 2143511. PMID 9007972.
• Kos J, Lah TT (1998). "Cysteine proteinases and their endogenous inhibitors: target proteins for prognosis, diagnosis and therapy in cancer (review)". Oncol. Rep. 5 (6): 1349–61. doi:10.3892/or.5.6.1349. PMID 9769367.
• Rinne A, Järvinen M, Räsänen O (1979). "A protein reminiscent of the epidermal SH-protease inhibitor occurs in squamous epithelia of man and rat". Acta Histochem. 63 (2): 183–92. doi:10.1016/s0065-1281(78)80024-5. PMID 107702.
• Räsänen O, Järvinen M, Rinne A (1979). "Localization of the human SH-protease inhibitor in the epidermis. Immunofluorescent studies". Acta Histochem. 63 (2): 193–6. doi:10.1016/s0065-1281(78)80025-7. PMID 107703.
• Rasmussen HH, van Damme J, Puype M, et al. (1993). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis. 13 (12): 960–9. doi:10.1002/elps.11501301199. PMID 1286667. S2CID 41855774.
• Madsen P, Rasmussen HH, Leffers H, et al. (1991). "Molecular cloning, occurrence, and expression of a novel partially secreted protein "psoriasin" that is highly up-regulated in psoriatic skin". J. Invest. Dermatol. 97 (4): 701–12. doi:10.1111/1523-1747.ep12484041. PMID 1940442.
• Hsieh WT, Fong D, Sloane BF, et al. (1991). "Mapping of the gene for human cysteine proteinase inhibitor stefin A, STF1, to chromosome 3cen-q21". Genomics. 9 (1): 207–9. doi:10.1016/0888-7543(91)90241-6. PMID 2004763.
• Rinne A, Järvinen M, Dorn A, et al. (1986). "[Low-molecular cysteine protease inhibitors in the human palatal tonsil]". Anatomischer Anzeiger. 161 (3): 215–30. PMID 2424340.
• Kartasova T, Cornelissen BJ, Belt P, van de Putte P (1987). "Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes". Nucleic Acids Res. 15 (15): 5945–62. doi:10.1093/nar/15.15.5945. PMC 306060. PMID 2442723.
• Takeda A, Kaji H, Nakaya K, et al. (1989). "Comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes". J. Biochem. 105 (6): 986–91. doi:10.1093/oxfordjournals.jbchem.a122792. PMID 2768224.
• Strauss M, Stollwerk J, Lenarcic B, et al. (1989). "Chemical synthesis of a gene for human stefin A and its expression in E. coli". Biol. Chem. Hoppe-Seyler. 369 (9): 1019–30. doi:10.1515/bchm3.1988.369.2.1019. PMID 3067731.
• Davies ME, Barrett AJ (1984). "Immunolocalization of human cystatins in neutrophils and lymphocytes". Histochemistry. 80 (4): 373–7. doi:10.1007/BF00495420. PMID 6429090. S2CID 13559202.
• Machleidt W, Borchart U, Fritz H, et al. (1984). "Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes". Hoppe-Seyler's Z. Physiol. Chem. 364 (11): 1481–6. doi:10.1515/bchm2.1983.364.2.1481. PMID 6689312.
• Söderström KO, Laato M, Wu P, et al. (1995). "Expression of acid cysteine proteinase inhibitor (ACPI) in the normal human prostate, benign prostatic hyperplasia and adenocarcinoma". Int. J. Cancer. 62 (1): 1–4. doi:10.1002/ijc.2910620102. PMID 7541394. S2CID 25265556.
• Tate S, Ushioda T, Utsunomiya-Tate N, et al. (1995). "Solution structure of a human cystatin A variant, cystatin A2-98 M65L, by NMR spectroscopy. A possible role of the interactions between the N- and C-termini to maintain the inhibitory active form of cystatin A". Biochemistry. 34 (45): 14637–48. doi:10.1021/bi00045a004. PMID 7578072.
• Martin JR, Craven CJ, Jerala R, et al. (1995). "The three-dimensional solution structure of human stefin A". J. Mol. Biol. 246 (2): 331–43. doi:10.1006/jmbi.1994.0088. PMID 7869384.
• Steinert PM, Marekov LN (1997). "Direct evidence that involucrin is a major early isopeptide cross-linked component of the keratinocyte cornified cell envelope". J. Biol. Chem. 272 (3): 2021–30. doi:10.1074/jbc.272.3.2021. PMID 8999895.

External links

• Cystatin: a protein that flips out! QUite Interesting PDB Structure article at PDBe
• The MEROPS online database for peptidases and their inhibitors: I25.001