DNA-formamidopyrimidine glycosylase
DNA-formamidopyrimidine glycosylase (EC 3.2.2.23, Fapy-DNA glycosylase, deoxyribonucleate glycosidase, 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase, formamidopyrimidine-DNA glycosylase, DNA-formamidopyrimidine glycosidase, Fpg protein) is an enzyme with systematic name DNA glycohydrolase (2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide releasing).[1] FPG is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA.[2] It was discovered by Zimbabwean scientist Christopher J. Chetsanga in 1975.[3]
| DNA-formamidopyrimidine glycosylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.2.2.23 | ||||||||
| CAS number | 78783-53-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme catalyses the following chemical reaction
- Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
This enzyme participates in processes leading to recovery from mutagenesis and/or cell death by alkylating agents.
References
- Boiteux S, O'Connor TR, Laval J (October 1987). "Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein". The EMBO Journal. 6 (10): 3177–83. PMC 553760. PMID 3319582.
- Serre1; Pereira De Jésus, K; Boiteux, S; Zelwer, C; Castaing, B; et al. (2002). "Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA". The EMBO Journal. 21 (12): 2854–2865. doi:10.1093/emboj/cdf304. PMC 126059. PMID 12065399.
- Chetsanga C.J., Lindahl T. (1979). "Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from Escherichia coli". Nucleic Acids Res. 6 (11): 3673–84. doi:10.1093/nar/6.11.3673. PMC 327965. PMID 386277.
External links
- DNA-formamidopyrimidine+glycosylase at the US National Library of Medicine Medical Subject Headings (MeSH)