ENTPD6

Ectonucleoside triphosphate diphosphohydrolase 6 is an enzyme that in humans is encoded by the ENTPD6 gene.[5][6]

ENTPD6
Identifiers
Aliases	ENTPD6, CD39L2, IL-6SAG, IL6ST2, NTPDase-6, dJ738P15.3, ectonucleoside triphosphate diphosphohydrolase 6 (putative), ectonucleoside triphosphate diphosphohydrolase 6
External IDs	OMIM: 603160 MGI: 1202295 HomoloGene: 68170 GeneCards: ENTPD6
Gene location (Human) Chr. Chromosome 20 (human)[1] Band 20p11.21 Start 25,195,693 bp[1] End 25,228,075 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 G3 Start 150,749,042 bp[2] End 150,771,675 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • nucleoside-triphosphatase activity • guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity • uridine-diphosphatase activity • hydrolase activity • nucleoside-diphosphatase activity Cellular component • integral component of membrane • cell surface • Golgi membrane • cell membrane • membrane • extracellular region • extracellular • Golgi apparatus Biological process • response to calcium ion • response to magnesium ion • nucleobase-containing small molecule catabolic process • metabolism Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	955	12497
Ensembl	ENSG00000197586	ENSMUSG00000033068
UniProt	O75354 Q5QPI7	Q3U0P5
RefSeq (mRNA)	NM_001114089 NM_001247 NM_001317941 NM_001322378 NM_001322379 NM_001322380 NM_001322381 NM_001322382 NM_001322383 NM_001322384 NM_001322385 NM_001322386 NM_001322387 NM_001322388 NM_001322389 NM_001322390 NM_001322391 NM_001322392 NM_001322393 NM_001322394 NM_001322395 NM_001322396 NM_001322397 NM_001322398	NM_172117 NM_001355068
RefSeq (protein)	NP_001107561 NP_001238 NP_001304870 NP_001309307 NP_001309308 NP_001309309 NP_001309310 NP_001309311 NP_001309312 NP_001309313 NP_001309314 NP_001309315 NP_001309316 NP_001309317 NP_001309318 NP_001309319 NP_001309320 NP_001309321 NP_001309322 NP_001309323 NP_001309324 NP_001309325 NP_001309326 NP_001309327	NP_742115 NP_001341997 NP_001357558 NP_001357559 NP_001357560
Location (UCSC)	Chr 20: 25.2 – 25.23 Mb	Chr 2: 150.75 – 150.77 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which is characteristic of NTPases.[6]

Model organisms

Model organisms have been used in the study of ENTPD6 function. A conditional knockout mouse line called Entpd6^{tm1a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.[7] Male and female animals underwent a standardized phenotypic screen[8] to determine the effects of deletion.[9][10][11][12] Additional screens performed: - In-depth immunological phenotyping[13]

Entpd6 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[8][13]
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Salmonella infection	Normal

References

1. GRCh38: Ensembl release 89: ENSG00000197586 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000033068 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chadwick BP, Frischauf AM (Jun 1998). "The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster". Genomics. 50 (3): 357–67. doi:10.1006/geno.1998.5317. PMID 9676430.
6. "Entrez Gene: ENTPD6 ectonucleoside triphosphate diphosphohydrolase 6 (putative function)".
7. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
8. "International Mouse Phenotyping Consortium".
9. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
12. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
13. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Chadwick BP, Frischauf AM (Sep 1997). "Cloning and mapping of a human and mouse gene with homology to ecto-ATPase genes". Mammalian Genome. 8 (9): 668–72. doi:10.1007/s003359900534. PMID 9271669. S2CID 42644202.
• Hicks-Berger CA, Chadwick BP, Frischauf AM, Kirley TL (Nov 2000). "Expression and characterization of soluble and membrane-bound human nucleoside triphosphate diphosphohydrolase 6 (CD39L2)". The Journal of Biological Chemistry. 275 (44): 34041–5. doi:10.1074/jbc.M004723200. PMID 10948193.
• Yeung G, Mulero JJ, McGowan DW, Bajwa SS, Ford JE (Oct 2000). "CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart". Biochemistry. 39 (42): 12916–23. doi:10.1021/bi000959z. PMID 11041856.
• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Ivanenkov VV, Murphy-Piedmonte DM, Kirley TL (Oct 2003). "Bacterial expression, characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function". Biochemistry. 42 (40): 11726–35. doi:10.1021/bi035137r. PMID 14529283.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (Oct 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Otsuki T, Ota T, Nishikawa T, Hayashi K, Suzuki Y, Yamamoto J, Wakamatsu A, Kimura K, Sakamoto K, Hatano N, Kawai Y, Ishii S, Saito K, Kojima S, Sugiyama T, Ono T, Okano K, Yoshikawa Y, Aotsuka S, Sasaki N, Hattori A, Okumura K, Nagai K, Sugano S, Isogai T (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Research. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (Jan 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (May 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.