Edward Kravitz

Edward Arthur Kravitz (born December 19, 1932) is the George Packer Berry Professor of Neurobiology at Harvard Medical School.[1] He is widely recognized for demonstrating that gamma-aminobutyric acid (GABA) functions as a neurotransmitter.[2] In addition, he and Antony Stretton were the first to use the intracellular dye procion yellow to visualize neuronal architecture.[3] Later, Kravitz's work with neuroamines demonstrated that serotonin and octopamine act as synaptic modulators. Kravitz continued to explore the function of amines using Homarus americanus, the American lobster, as a model organism to study aggression. He currently works on aggressive behavior using the genetically manipulable model organism, Drosophila melanogaster, the fruit fly.

Edward Kravitz
Born(1932-12-19)December 19, 1932
New York City
Alma materCity College of New York
University of Michigan
Known forIdentifying gamma-aminobutyric acid (GABA) as a neurotransmitter
Awards
Scientific career
FieldsNeuroscience
InstitutionsNational Institute of Health
Harvard Medical School
Doctoral studentsMargaret Livingstone
Thomas Schwarz

Personal life

Ed Kravitz was born in New York to Ada Machlus and Isadore Kravitz. He has one older brother, Bill, born in 1929. Kravitz grew up in The Bronx during the Great Depression. More than once he skipped an entire grade in order to be challenged in school and ended up in college at age 16.[4] Ed met his wife Kathryn Anne Frakes at the University of Michigan; they were married in 1959. Together they had two sons, David[5] (b. February 21, 1964) and James[6] (b. May 14, 1966).

Scientific career

After graduating from Evander Childs High School in The Bronx, Kravitz remained in the neighborhood he grew up in and began his studies at City College of New York (CCNY). In 1954 he graduated from CCNY with a double major in Biology and Chemistry. Unsure of what to do next, Kravitz applied to be an officer in the U.S. Army Medical Corps as well as to two medical schools, and for a Research Assistant position. He ended up at Sloan-Kettering in the laboratory of George Tarnowski. Under the supervision of George Tarnowski, Lou Kaplan, a young biochemist at the time, and Christine Riley, director of the chemotherapy unit, Kravitz began an independent research project studying amino acid metabolism in ascites tumor cells. It was this experience that led to Kravitz's decision to pursue a career as a Scientist. In 1954, Kravitz began graduate school at the University of Michigan. He met a lot of great colleagues at this time, including Marshall Nirenberg with whom he shared an apartment on Huron Avenue in Ann Arbor. Kravitz's thesis work was done in the laboratory of Armand Guarino and led to his first paper “On the effect of inorganic phosphate on hexose phosphate metabolism” which was published in the journal Science. In 1959 he received his Ph.D. in Biological Chemistry and began working in Earl Stadtman's laboratory at the National Institutes of Health. Although at one time Kravitz planned on pursuing two additional post-doctoral positions after studying morphine metabolism in the Stadtman laboratory, he was recruited to Harvard Medical School by Steve Kuffler in 1960. Almost immediately, he began working with Steve Kuffler, Dave Potter and Nico van Gelder on the experiments that would eventually demonstrate that GABA functions as a neurotransmitter. From his biochemistry training and friends at NIH, Kravitz knew that by growing Pseudomonas fluorescens on GABA as a sole carbon source, an enzymatic assay could be used to quantify the amount of GABA in the neurons of crustaceans. Using this enzymatic assay, the group quickly learned that GABA was highly expressed in inhibitory neurons. Later Kravitz worked with Masanori Otsuka, Les Iversen, and Zach Hall to show that GABA was released from inhibitory neurons of lobsters.[7] While today Ed's work on GABA is well respected, it was quite controversial when first presented publicly. After his first talk on the work at the Marine Biological Laboratory in Woods Hole, David Nachmanson commented “Well, we don’t know what that little bit of an amino acid that you see being released is when you stimulate a nerve, but it certainly is not a chemical transmitter compound, because we all know that transmission is electrical”.[8]

The second project Kravtiz took on in the mid-1960s was much more anatomical in nature. In collaboration with his postdoctoral fellow, Antony Stretton, Ed began developing a technique to visualize the structure of neurons in order to determine whether neuronal shapes are genetically specified. Two other Scientists at Harvard Medical School, Ed Furshpan and Jaime Alvarez, had been using intracellular dyes to localize their recording electrodes in the brains of fish, but none of their dyes were able to stain the neuropil processes of the injected neurons. Kravitz and Stretton contacted Imperial Chemicals, a manufacturer of fabric staining dyes located in Providence, RI and obtained over 120 dyes to inject into lobster neurons. In the end they found a single dye, Procion Yellow, that was highly soluble, readily released from microelectrodes, completely filled cells and their processes, survived fixation and dehydration, and, most importantly, was fluorescent. Using Procion Yellow, Kravitz, Stretton, and Edith Maier found that neurons from two different animals had strikingly similar morphological shapes. They eventually injected over 100 physiologically identified neurons, processed and sectioned the ganglia, and reconstructed the cell shapes by hand from photographs of the serial sections.[9]

In the 1970s Kravitz's laboratory turned their focus back to neurotransmitters. After finding evidence that glutamate acts as an excitatory transmitter in crustaceans, they found that acetylcholine functions as the lobster sensory transmitter compound. Around this time, the laboratory also began experimenting with the neuroamines serotonin and octopamine. By trying to understand how naturally occurring neuromodulators might act, Margaret Livingstone, a graduate student at the time, injected serotonin or octopamine into two different lobsters. The results were surprising: the lobster injected with serotonin stood tall and looked just like a dominant animal while the lobster injected with octopamine adopted a lowered posture and looked like a subordinate animal.[10] These lobster injection experiments were the birth of the aggressive behavior studies that are still ongoing in Kravitz's laboratory today.

In the 1980s and 1990s Kravitz's laboratory evolved into a neuroethology laboratory. In collaboration with his postdoctoral fellow Robert Huber, a quantitative analysis of lobster fighting behavior was underway. Lobsters proved to be an excellent model system for studies on aggression due to the ease in getting animals to fight and the fact that anatomical and physiological studies were possible. However, Kravitz soon realized that in order to discover new neurons and pathways that were important for aggression, he needed an organism whose genome was sequenced and where genetic methods were available for solving sophisticated problems, leading to work with Drosophila melanogaster.

Honors and awards (partial list)

References
  1. "Edward Kravitz - Department of Neurobiology". neuro.hms.harvard.edu.
  2. Gelder, N. M. Van; Potter, D. D.; Kravitz, E. A. (1 April 1962). "Gamma-Aminobutyric Acid and Other Blocking Substances extracted from Crab Muscle". Nature. 194 (4826): 382–383. doi:10.1038/194382b0. PMID 14459471.
  3. Kravitz, E. A.; Stretton, A. O. W. (4 October 1968). "Neuronal Geometry: Determination with a Technique of Intracellular Dye Injecion". Science. 162 (3849): 132–134. doi:10.1126/science.162.3849.132. PMID 4175300.
  4. Camhi, J. (1 March 2000). "Introduction to the King Solomon Lecture of Edward Kravitz". Journal of Comparative Physiology A. 186 (3): 219–220. doi:10.1007/s003590050422.
  5. "David Kravitz, Baritone". www.davidkravitz.com.
  6. "Jamie Kravitz, UX Designer - Portfolio - Wireframes, Prototypes, Usability Testing, Evaluation, Research". www.digivitz.com.
  7. "Otsuka M., Iversen L.L., Hall Z.W., Kravitz E.A. 1966. Release of gamma-aminobutyric acid from inhibitory nerves of lobster. Proc Natl Acad Sci U S A. 56: p.1110-5". nih.gov.
  8. "Kravitz, E.A. 2003. My life up to now. In Squire L. (ed) The History of Neuroscience in Autobiography, Volume 4. Academic Press, NY p.280-343". elsevier.com.
  9. International Society for Neuroethology Newsletter March 2000 Archived 2007-08-16 at the Wayback Machine
  10. Kravitz, Edward A.; Harris-Warrick, Ronald M.; Livingstone, Margaret S. (4 April 1980). "Serotonin and Octopamine Produce Opposite Postures in Lobsters". Science. 208 (4439): 76–79. doi:10.1126/science.208.4439.76. PMID 17731572.
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