F-tractin

F-Tractin is a cellular probe for filamentous actin. It consists of a portion of the amino terminal actin binding region of the rat protein ITPKA, usually fused to a reporter such as green fluorescent protein. Initial studies determined that amino acids 9-52 from the rat ITPKA were useful as a live-cell reporter for actin filaments. Later studies determined that amino acids 9-40 were sufficient for F-actin binding.[1][2][3] F-Tractin is one of a number of F-actin probes useful in live cell imaging.[4]

References
  1. Schell MJ, Erneux C, Irvine RF (2001). "Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus". J Biol Chem. 276 (40): 37537–46. doi:10.1074/jbc.M104101200. PMID 11468283.
  2. Johnson HW, Schell MJ (2009). "Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology". Mol Biol Cell. 20 (24): 5166–80. doi:10.1091/mbc.E09-01-0083. PMC 2793293. PMID 19846664.
  3. Yi J, Wu XS, Crites T, Hammer JA (2012). "Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells". Mol Biol Cell. 23 (5): 834–52. doi:10.1091/mbc.E11-08-0731. PMC 3290643. PMID 22219382.
  4. Melak M, Plessner M, Grosse R (2017). "Actin visualization at a glance". J Cell Sci. 130 (3): 525–530. doi:10.1242/jcs.189068. PMID 28082420.


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