H295R

H295R (also referred to as NCI-H295R) is an angiotensin-II-responsive steroid-producing adrenocortical cell line.[1] It was initially isolated in 1980 from a 48-year-old female patient diagnosed with adrenocortical carcinoma.[1][2] The initial polyclonal populations of tumor cells obtained from the patients' tumor were cultured and the resultant cell line was called NCI-H295.[1][2] Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth.[1] Three strains were developed, based on the serum supplement used for growth, which have been termed H295R-S1, H295R-S2 and H295R-S3.[1][3] All three strains grow as adherent monolayer cultures.[1]

References
  1. Wang T, Rainey WE (2012). "Human Adrenocortical Carcinoma Cell Lines". Mol. Cell. Endocrinol. 351 (1): 58–65. doi:10.1016/j.mce.2011.08.041. PMC 3288152. PMID 21924324.
  2. Gazdar AF, Oie HK, Shackleton CH, Chen TR, Triche TJ, Myers CE, Chrousos GP, Brennan MF, Stein CA, La Rocca RV (1990). "Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis". Cancer Res. 50 (17): 5488–5496. PMID 2386954.
  3. Rainey WE, Saner K, Schimmer BP (2004). "Adrenocortical cell lines". Mol. Cell. Endocrinol. 228 (1–2): 23–38. doi:10.1016/j.mce.2003.12.020. PMID 15541570.
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