IWS1

Protein IWS1 homolog also known as interacts with Spt6 (IWS1) is a protein that in humans is encoded by the IWS1 gene.[5]

IWS1
Identifiers
Aliases	IWS1, SUPT6H interacting protein, interacts with SUPT6H, CTD assembly factor 1
External IDs	MGI: 1920723 HomoloGene: 134421 GeneCards: IWS1
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q14.3 Start 127,436,207 bp[1] End 127,526,886 bp[1]
Gene location (Mouse) Chr. Chromosome 18 (mouse)[2] Band 18|18 B1 Start 32,067,741 bp[2] End 32,104,328 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding Cellular component • cell nucleus • nucleoplasm Biological process • regulation of transcription, DNA-templated • mRNA transport • RNA splicing • regulation of histone H4 acetylation • regulation of mRNA processing • mRNA processing • regulation of mRNA export from nucleus • regulation of histone H3-K36 trimethylation • transcription, DNA-templated • transcription from RNA polymerase II promoter • transcription elongation from RNA polymerase II promoter • regulation of DNA-templated transcription, elongation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	55677	73473
Ensembl	ENSG00000163166	ENSMUSG00000024384
UniProt	Q96ST2	Q8C1D8
RefSeq (mRNA)	NM_017969	NM_173441
RefSeq (protein)	NP_060439	NP_775617
Location (UCSC)	Chr 2: 127.44 – 127.53 Mb	Chr 18: 32.07 – 32.1 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

IWS1 is a transcription elongation factor. It was first identified during a search for RNA polymerase II-associated elongation factors in yeast; it directly interacts with RNA polymerase II (RNAPII) and is phosphorylated at casein kinase II (CKII) sites.[6]

The human homolog, which physically interacts with protein arginine methyltransferase 5 (PRMT5), is essential for cell survival.[7] It also recruits a SET2 histone methyltransferase (Huntingtin-interacting protein HYPB, also known as SETD2) to RNAPII during transcription elongation and is required for H3K36 trimethylation.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000163166 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024384 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: IWS1 IWS1 homolog (S. cerevisiae)".
6. Krogan NJ, Kim M, Ahn SH, Zhong G, Kobor MS, Cagney G, Emili A, Shilatifard A, Buratowski S, Greenblatt JF (Oct 2002). "RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach". Molecular and Cellular Biology. 22 (20): 6979–92. doi:10.1128/MCB.22.20.6979-6992.2002. PMC 139818. PMID 12242279.
7. Liu Z, Zhou Z, Chen G, Bao S (Feb 2007). "A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation". Biochemical and Biophysical Research Communications. 353 (1): 47–53. doi:10.1016/j.bbrc.2006.11.133. PMID 17184735.
8. Yoh SM, Lucas JS, Jones KA (Dec 2008). "The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation". Genes & Development. 22 (24): 3422–34. doi:10.1101/gad.1720008. PMC 2607075. PMID 19141475.

Further reading

• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (Apr 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Ling Y, Smith AJ, Morgan GT (2006). "A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS". Nucleic Acids Research. 34 (8): 2219–29. doi:10.1093/nar/gkl239. PMC 1450333. PMID 16648364.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (Oct 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nature Biotechnology. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Liu Z, Zhou Z, Chen G, Bao S (Feb 2007). "A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation". Biochemical and Biophysical Research Communications. 353 (1): 47–53. doi:10.1016/j.bbrc.2006.11.133. PMID 17184735.
• Yoh SM, Cho H, Pickle L, Evans RM, Jones KA (Jan 2007). "The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export". Genes & Development. 21 (2): 160–74. doi:10.1101/gad.1503107. PMC 1770899. PMID 17234882.