MAGI1

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 is an enzyme that in humans is encoded by the MAGI1 gene.[5][6][7]

MAGI1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1WBP, 2KPK, 2KPL, 2Q9V, 2R4H, 2YSD, 2YSE, 2ZAJ, 3BPU
Identifiers
Aliases	MAGI1, AIP-3, AIP3, BAIAP1, BAP-1, BAP1, MAGI-1, Magi1d, TNRC19, WWP3, membrane associated guanylate kinase, WW and PDZ domain containing 1, MAGI-1b
External IDs	OMIM: 602625 MGI: 1203522 HomoloGene: 31257 GeneCards: MAGI1
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p14.1 Start 65,353,525 bp[1] End 66,038,834 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6|6 D1 Start 93,675,455 bp[2] End 94,283,917 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0032947 molecular adaptor activity • nucleotide binding • protein C-terminus binding • GO:0001948 protein binding • ATP binding • alpha-actinin binding Cellular component • adherens junction • cell junction • cell membrane • cell projection • membrane • intercellular junction • nucleoplasm • bicellular tight junction • cell nucleus • cytoplasm Biological process • neuron death • cell surface receptor signaling pathway • cell adhesion • macromolecular complex assembly • signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9223	14924
Ensembl	ENSG00000282956 ENSG00000151276	ENSMUSG00000045095
UniProt	Q96QZ7	Q6RHR9
RefSeq (mRNA)	NM_001033057 NM_004742 NM_015520 NM_001365903 NM_001365904 NM_001365905	NM_001029850 NM_001083320 NM_001083321 NM_001286784 NM_001286785 NM_001286786 NM_001286788 NM_010367
RefSeq (protein)	NP_001028229 NP_004733 NP_056335 NP_001352832 NP_001352833 NP_001352834	NP_001025021 NP_001076789 NP_001076790 NP_001273713 NP_001273714 NP_001273715 NP_001273717 NP_034497
Location (UCSC)	Chr 3: 65.35 – 66.04 Mb	Chr 6: 93.68 – 94.28 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell–cell contact. The product of this gene may play a role as scaffolding protein at cell–cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified.[7]

Interactions

MAGI1 has been shown to interact with:

• ACCN3,[8]
• ATN1,[9]
• Actinin alpha 4,[10]
• Beta-catenin,[11]
• Brain-specific angiogenesis inhibitor 1,[5]
• Calcium-activated potassium channel subunit alpha-1,[12]
• FCHSD2,[13]
• LRP2,[14] and
• SYNPO.[10]

References

1. ENSG00000151276 GRCh38: Ensembl release 89: ENSG00000282956, ENSG00000151276 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000045095 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Shiratsuchi T, Futamura M, Oda K, Nishimori H, Nakamura Y, Tokino T (Jun 1998). "Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1". Biochemical and Biophysical Research Communications. 247 (3): 597–604. doi:10.1006/bbrc.1998.8603. PMID 9647739.
6. Margolis RL, Abraham MR, Gatchell SB, Li SH, Kidwai AS, Breschel TS, Stine OC, Callahan C, McInnis MG, Ross CA (Jul 1997). "cDNAs with long CAG trinucleotide repeats from human brain". Human Genetics. 100 (1): 114–22. doi:10.1007/s004390050476. PMID 9225980. S2CID 25999127.
7. "Entrez Gene: MAGI1 membrane associated guanylate kinase, WW and PDZ domain containing 1".
8. Hruska-Hageman AM, Benson CJ, Leonard AS, Price MP, Welsh MJ (Nov 2004). "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current". The Journal of Biological Chemistry. 279 (45): 46962–8. doi:10.1074/jbc.M405874200. PMID 15317815.
9. Wood JD, Yuan J, Margolis RL, Colomer V, Duan K, Kushi J, Kaminsky Z, Kleiderlein JJ, Sharp AH, Ross CA (Jun 1998). "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins". Molecular and Cellular Neurosciences. 11 (3): 149–60. doi:10.1006/mcne.1998.0677. PMID 9647693. S2CID 20003277.
10. Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B (Aug 2002). "Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1". The Journal of Biological Chemistry. 277 (33): 30183–90. doi:10.1074/jbc.M203072200. PMID 12042308.
11. Dobrosotskaya IY, James GL (Apr 2000). "MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures". Biochemical and Biophysical Research Communications. 270 (3): 903–9. doi:10.1006/bbrc.2000.2471. PMID 10772923.
12. Ridgway LD, Kim EY, Dryer SE (Jul 2009). "MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface". American Journal of Physiology. Cell Physiology. 297 (1): C55–65. doi:10.1152/ajpcell.00073.2009. PMC 3774261. PMID 19403801.
13. Ohno H, Hirabayashi S, Kansaku A, Yao I, Tajima M, Nishimura W, Ohnishi H, Mashima H, Fujita T, Omata M, Hata Y (Nov 2003). "Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains". Oncogene. 22 (52): 8422–31. doi:10.1038/sj.onc.1206996. PMID 14627983.
14. Patrie KM, Drescher AJ, Goyal M, Wiggins RC, Margolis B (Apr 2001). "The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes". Journal of the American Society of Nephrology. 12 (4): 667–77. PMID 11274227.

Further reading

• Pirozzi G, McConnell SJ, Uveges AJ, Carter JM, Sparks AB, Kay BK, Fowlkes DM (Jun 1997). "Identification of novel human WW domain-containing proteins by cloning of ligand targets". The Journal of Biological Chemistry. 272 (23): 14611–6. doi:10.1074/jbc.272.23.14611. PMID 9169421.
• Dobrosotskaya I, Guy RK, James GL (Dec 1997). "MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains". The Journal of Biological Chemistry. 272 (50): 31589–97. doi:10.1074/jbc.272.50.31589. PMID 9395497.
• Wood JD, Yuan J, Margolis RL, Colomer V, Duan K, Kushi J, Kaminsky Z, Kleiderlein JJ, Sharp AH, Ross CA (Jun 1998). "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins". Molecular and Cellular Neurosciences. 11 (3): 149–60. doi:10.1006/mcne.1998.0677. PMID 9647693. S2CID 20003277.
• Dobrosotskaya IY, James GL (Apr 2000). "MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures". Biochemical and Biophysical Research Communications. 270 (3): 903–9. doi:10.1006/bbrc.2000.2471. PMID 10772923.
• Patrie KM, Drescher AJ, Goyal M, Wiggins RC, Margolis B (Apr 2001). "The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes". Journal of the American Society of Nephrology. 12 (4): 667–77. PMID 11274227.
• Dobrosotskaya IY (May 2001). "Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1". Biochemical and Biophysical Research Communications. 283 (4): 969–75. doi:10.1006/bbrc.2001.4880. PMID 11350080.
• Laura RP, Ross S, Koeppen H, Lasky LA (May 2002). "MAGI-1: a widely expressed, alternatively spliced tight junction protein". Experimental Cell Research. 275 (2): 155–70. doi:10.1006/excr.2002.5475. PMID 11969287.
• Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B (Aug 2002). "Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1". The Journal of Biological Chemistry. 277 (33): 30183–90. doi:10.1074/jbc.M203072200. PMID 12042308.
• Ohno H, Hirabayashi S, Kansaku A, Yao I, Tajima M, Nishimura W, Ohnishi H, Mashima H, Fujita T, Omata M, Hata Y (Nov 2003). "Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains". Oncogene. 22 (52): 8422–31. doi:10.1038/sj.onc.1206996. PMID 14627983.
• Hruska-Hageman AM, Benson CJ, Leonard AS, Price MP, Welsh MJ (Nov 2004). "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current". The Journal of Biological Chemistry. 279 (45): 46962–8. doi:10.1074/jbc.M405874200. PMID 15317815.
• Wright GJ, Leslie JD, Ariza-McNaughton L, Lewis J (Nov 2004). "Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development". Development. 131 (22): 5659–69. doi:10.1242/dev.01417. PMID 15509766.
• Benzinger A, Muster N, Koch HB, Yates JR, Hermeking H (Jun 2005). "Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer". Molecular & Cellular Proteomics. 4 (6): 785–95. doi:10.1074/mcp.M500021-MCP200. PMID 15778465.
• Mizuhara E, Nakatani T, Minaki Y, Sakamoto Y, Ono Y, Takai Y (Jul 2005). "MAGI1 recruits Dll1 to cadherin-based adherens junctions and stabilizes it on the cell surface". The Journal of Biological Chemistry. 280 (28): 26499–507. doi:10.1074/jbc.M500375200. PMID 15908431.
• Patrie KM (Aug 2005). "Identification and characterization of a novel tight junction-associated family of proteins that interacts with a WW domain of MAGI-1". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1745 (1): 131–44. doi:10.1016/j.bbamcr.2005.05.011. PMID 16019084.
• Bratt A, Birot O, Sinha I, Veitonmäki N, Aase K, Ernkvist M, Holmgren L (Oct 2005). "Angiomotin regulates endothelial cell-cell junctions and cell motility". The Journal of Biological Chemistry. 280 (41): 34859–69. doi:10.1074/jbc.M503915200. PMID 16043488.
• Sakurai A, Fukuhara S, Yamagishi A, Sako K, Kamioka Y, Masuda M, Nakaoka Y, Mochizuki N (Feb 2006). "MAGI-1 is required for Rap1 activation upon cell-cell contact and for enhancement of vascular endothelial cadherin-mediated cell adhesion". Molecular Biology of the Cell. 17 (2): 966–76. doi:10.1091/mbc.E05-07-0647. PMC 1356604. PMID 16339077.