MAGI2

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 also known as membrane-associated guanylate kinase inverted 2 (MAGI-2) and atrophin-1-interacting protein 1 (AIP-1) is an enzyme that in humans is encoded by the MAGI2 gene.[5][6][7]

MAGI2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1UEP, 1UEQ, 1UEW, 1UJV, 1WFV
Identifiers
Aliases	MAGI2, ACVRIP1, AIP-1, AIP1, ARIP1, MAGI-2, SSCAM, membrane associated guanylate kinase, WW and PDZ domain containing 2, NPHS15
External IDs	OMIM: 606382 MGI: 1354953 HomoloGene: 8189 GeneCards: MAGI2
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7q21.11 Start 78,017,055 bp[1] End 79,453,667 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 A3 Start 19,227,036 bp[2] End 20,704,792 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • signal transducer activity • SMAD binding • phosphatase binding • beta-1 adrenergic receptor binding • GO:0032947 molecular adaptor activity • type II activin receptor binding • receptor signaling complex scaffold activity • GO:0001948 protein binding • activin receptor binding • kinase activity Cellular component • late endosome • cytoplasm • synapse • cell junction • cell nucleus • neuron projection • GO:0097483, GO:0097481 postsynaptic density • membrane • dendrite • perinuclear region of cytoplasm • slit diaphragm • bicellular tight junction • cell membrane • endosome • macromolecular complex • intercellular junction Biological process • receptor clustering • negative regulation of cell proliferation • positive regulation of neuron projection development • mitotic cell cycle arrest • neuroligin clustering involved in postsynaptic membrane assembly • signal transduction • negative regulation of activin receptor signaling pathway • nerve growth factor signaling pathway • planar cell polarity pathway involved in axis elongation • SMAD protein signal transduction • positive regulation of receptor internalization • protein heterooligomerization • negative regulation of protein kinase B signaling • nervous system development • glomerular visceral epithelial cell development • positive regulation of synaptic vesicle clustering • cellular response to nerve growth factor stimulus • positive regulation of phosphoprotein phosphatase activity • negative regulation of cell migration • positive regulation of signal transduction • phosphorylation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9863	50791
Ensembl	ENSG00000187391	ENSMUSG00000040003
UniProt	Q86UL8	Q9WVQ1
RefSeq (mRNA)	NM_001301128 NM_012301	NM_001170745 NM_001170746 NM_015823
RefSeq (protein)	NP_001288057 NP_036433	NP_001164216 NP_001164217 NP_056638
Location (UCSC)	Chr 7: 78.02 – 79.45 Mb	Chr 5: 19.23 – 20.7 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral-pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family.[7]

Interactions

MAGI2 has been shown to interact with ATN1[8] and PTEN (gene).

References

1. GRCh38: Ensembl release 89: ENSG00000187391 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040003 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Shoji H, Tsuchida K, Kishi H, Yamakawa N, Matsuzaki T, Liu Z, Nakamura T, Sugino H (Mar 2000). "Identification and characterization of a PDZ protein that interacts with activin type II receptors". J Biol Chem. 275 (8): 5485–92. doi:10.1074/jbc.275.8.5485. PMID 10681527.
6. Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Dec 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
7. "Entrez Gene: MAGI2 membrane associated guanylate kinase, WW and PDZ domain containing 2".
8. Wood, J D; Yuan J; Margolis R L; Colomer V; Duan K; Kushi J; Kaminsky Z; Kleiderlein J J; Sharp A H; Ross C A (Jun 1998). "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins". Mol. Cell. Neurosci. United States. 11 (3): 149–60. doi:10.1006/mcne.1998.0677. ISSN 1044-7431. PMID 9647693. S2CID 20003277.

Further reading

• Wood JD, Yuan J, Margolis RL, et al. (1998). "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins". Mol. Cell. Neurosci. 11 (3): 149–60. doi:10.1006/mcne.1998.0677. PMID 9647693. S2CID 20003277.
• Sanger Centre, The; Washington University Genome Sequencing Cente, The (1999). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.
• Ide N, Hata Y, Deguchi M, et al. (1999). "Interaction of S-SCAM with neural plakophilin-related Armadillo-repeat protein/delta-catenin". Biochem. Biophys. Res. Commun. 256 (3): 456–61. doi:10.1006/bbrc.1999.0364. PMID 10080919.
• Wu X, Hepner K, Castelino-Prabhu S, et al. (2000). "Evidence for regulation of the PTEN tumor suppressor by a membrane-localized multi-PDZ domain containing scaffold protein MAGI-2". Proc. Natl. Acad. Sci. U.S.A. 97 (8): 4233–8. Bibcode:2000PNAS...97.4233W. doi:10.1073/pnas.97.8.4233. PMC 18208. PMID 10760291.
• Xu J, Paquet M, Lau AG, et al. (2001). "beta 1-adrenergic receptor association with the synaptic scaffolding protein membrane-associated guanylate kinase inverted-2 (MAGI-2). Differential regulation of receptor internalization by MAGI-2 and PSD-95". J. Biol. Chem. 276 (44): 41310–7. doi:10.1074/jbc.M107480200. PMID 11526121.
• Vazquez F, Grossman SR, Takahashi Y, et al. (2002). "Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex". J. Biol. Chem. 276 (52): 48627–30. doi:10.1074/jbc.C100556200. PMID 11707428.
• Nishimura W, Yao I, Iida J, et al. (2002). "Interaction of synaptic scaffolding molecule and Beta -catenin". J. Neurosci. 22 (3): 757–65. doi:10.1523/JNEUROSCI.22-03-00757.2002. PMC 6758496. PMID 11826105.
• Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Yap CC, Muto Y, Kishida H, et al. (2003). "PKC regulates the delta2 glutamate receptor interaction with S-SCAM/MAGI-2 protein". Biochem. Biophys. Res. Commun. 301 (4): 1122–8. doi:10.1016/S0006-291X(03)00070-6. PMID 12589829.
• Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767–72. Bibcode:2003Sci...300..767S. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205.
• Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–64. Bibcode:2003Natur.424..157H. doi:10.1038/nature01782. PMID 12853948.
• Evgrafov OV, Mersiyanova I, Irobi J, et al. (2004). "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy". Nat. Genet. 36 (6): 602–6. doi:10.1038/ng1354. PMID 15122254.
• Franklin JL, Yoshiura K, Dempsey PJ, et al. (2005). "Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein". Exp. Cell Res. 303 (2): 457–70. doi:10.1016/j.yexcr.2004.10.007. PMID 15652357.
• Hu Y, Li Z, Guo L, et al. (2007). "MAGI-2 Inhibits cell migration and proliferation via PTEN in human hepatocarcinoma cells". Arch. Biochem. Biophys. 467 (1): 1–9. doi:10.1016/j.abb.2007.07.027. PMID 17880912.