MKI67IP

MKI67 FHA domain-interacting nucleolar phosphoprotein is a protein that in humans is encoded by the MKI67IP gene.[5][6]

NIFK
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AFF
Identifiers
Aliases	NIFK, MKI67IP, Nopp34, nucleolar protein interacting with the FHA domain of MKI67
External IDs	OMIM: 611970 MGI: 1915199 HomoloGene: 49862 GeneCards: NIFK
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q14.3 Start 121,726,945 bp[1] End 121,736,911 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 E2.3 Start 118,321,839 bp[2] End 118,333,822 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • RNA binding • nucleic acid binding Cellular component • cytoplasm • condensed nuclear chromosome • chromosome • nucleolus • cell nucleus • nucleoplasm Biological process • negative regulation of phosphatase activity • rRNA transcription • rRNA metabolic process • macromolecular complex assembly Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	84365	67949
Ensembl	ENSG00000155438	ENSMUSG00000026377
UniProt	Q9BYG3	Q91VE6
RefSeq (mRNA)	NM_032390	NM_026472
RefSeq (protein)	NP_115766	NP_080748
Location (UCSC)	Chr 2: 121.73 – 121.74 Mb	Chr 1: 118.32 – 118.33 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

FHA Ki67 binding domain of hNIFK
the solution structure of the ki67fha/hnifk(226-269)3p complex
Identifiers
Symbol	hNIFK_binding
Pfam	PF12196
InterPro	IPR021043
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

MKI67 FHA domain-interacting nucleolar phosphoprotein contains an RNA recognition motif (RRM) near to the N-terminus and a FHA Ki67 binding domain near to the C-terminus. There are two conserved sequence motifs within the FHA Ki67 binding domain: TPVCTP and LERRKS, this domain binds to the forkhead-associated domain of human Ki67. High-affinity binding requires sequential phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238, pThr234 and pSer230. This interaction is involved in cell cycle regulation.[7]

References

1. GRCh38: Ensembl release 89: ENSG00000155438 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026377 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Takagi M, Sueishi M, Saiwaki T, Kametaka A, Yoneda Y (Jul 2001). "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis". J Biol Chem. 276 (27): 25386–91. doi:10.1074/jbc.M102227200. PMID 11342549.
6. "Entrez Gene: MKI67IP MKI67 (FHA domain) interacting nucleolar phosphoprotein".
7. Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD (November 2005). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID 16244663. S2CID 5680428.

Further reading

• Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Li H, Byeon IJ, Ju Y, Tsai MD (2004). "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions". J. Mol. Biol. 335 (1): 371–81. doi:10.1016/j.jmb.2003.10.032. PMID 14659764.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Byeon IJ, Li H, Song H, et al. (2006). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID 16244663. S2CID 5680428.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human MKI67 FHA domain-interacting nucleolar phosphoprotein (MKI67IP)

This article incorporates text from the public domain Pfam and InterPro: IPR021043