Meiotic recombination checkpoint

The meiotic recombination checkpoint monitors meiotic recombination during meiosis, and blocks the entry into metaphase I if recombination is not efficiently processed.

Spo11 catalyzes a double strand break (DSB) in one of the two homologous chromosomes to induce meiotic recombination. The repair of these DSBs are monitored at a DSB-dependent meiotic recombination checkpoint while at the DSB-independent meiotic recombination checkpoint the asynapsis of each homolog pair is examined.

Generally speaking, the cell cycle regulation of meiosis is similar to that of mitosis. As in the mitotic cycle, these transitions are regulated by combinations of different gene regulatory factors, the cyclin-Cdk complex and the anaphase-promoting complex (APC).[1] The first major regulatory transition occurs in late G1, when the start of meiotic cycle is activated by Ime1 instead of Cln3/Cdk1 in mitosis. The second major transition occurs at the entry into metaphase I. The main purpose of this step is to make sure that DNA replication has completed without error so that spindle pole bodies can separate. This event is triggered by the activation of M-Cdk in late prophase I. Then the spindle assembly checkpoint examines the attachment of microtubules at kinetochores, followed by initiation of metaphase I by APCCdc20. The special chromosome separation in meiosis, homologous chromosomes separation in meiosis I and chromatids separation in meiosis II, requires special tension between homologous chromatids and non-homologous chromatids for distinguishing microtubule attachment and it relies on the programmed DNA double strand break (DSB) and repair in prophase I. Therefore meiotic recombination checkpoint can be a kind of DNA damage response at specific time spot. On the other hand, the meiotic recombination checkpoint also makes sure that meiotic recombination does happen in every pair of homologs.

DSB-dependent pathway

The abrupt onset of M-Cdk in late prophase I depends on the positive transcription regulation feedback loop consisting of Ime2, Ndt80 and Cdk/cyclin complex. However the activation of M-Cdk is controlled by the general phosphorylation switch Wee1/Cdc25. Wee1 activity is high in early prophase I and the accumulation of Cdc25 activates M-Cdk by direct phosphorylation and marking Wee1 to be degraded. Meiotic recombination may begin with a double-strand break, either induced by Spo11[2] or by other endogenous or exogenous causes of DNA damage. These DNA breaks must be repaired before metaphase I. and these DSBs must be repaired before metaphase I. The cell monitor these DSBs via ATM pathway, in which Cdc25 is suppressed when DSB lesion is detected. This pathway is the same as classical DNA damage response and is the part we know the best in meiotic recombination checkpoint.

DSB-independent pathway

The DSB-independent pathway was proposed when people studied spo11 mutant cells in some species and found that these Spo11 cells could not process to metaphase I even in the absence of DSB.[3] The direct purpose of these DSBs is to help with the condensation of chromosomes. Even though the initial homolog paring in early leptotene is just random interactions, the further progression into presynaptic alignment depends on the formation of double strand breaks and single strand transfer complexes.[1][4] Therefore the unsynapsed chromosomes in Spo11 cells can be a target of checkpoint. An AAA–adenosine triphosphatase (AAA-ATPase) was found to be essential in this pathway.[5] but the mechanism is not yet clear. Some other studies also drew sex body formation into attention, and the signaling could be either structure based or transcription regulation such as meiotic sex chromosome inactivation.[6][7] Under this cascade, failure to synapse will maintain the gene expression from sex chromosomes and some products may inhibit cell cycle progression. Meiotic sex chromosome inactivation only happens in male, which may partially be the reason why only Spo11 mutant spermatocytes but not oocytes fail to transition from prophase I to metaphase I.[3][8] However the asynapsis does not happen only within sex chromosomes, and such transcription regulation was suspended until it was further expanded to all the chromosomes as meiotic silencing of unsynapsed chromatin,[9] but the effector gene is not found yet.

Meiotic checkpoint protein kinases CHEK1 and CHEK2

The central role in meiosis of human and mouse CHEK1 and CHEK2 and their orthologs in Saccharomyces cerevisiae, Caenorhabditis elegans, Schizosaccharomyces pombe and Drosophila has been reviewed by MacQueen and Hochwagen[10] and Subramanian and Hochwagen.[11] During meiotic recombination in human and mouse, CHEK1 protein kinase is important for integrating DNA damage repair with cell cycle arrest.[12] CHEK1 is expressed in the testes and associates with meiotic synaptonemal complexes during the zygonema and pachynema stages.[12] CHEK1 likely acts as an integrator for ATM and ATR signals and in monitoring meiotic recombination.[12] In mouse oocytes CHEK1 appears to be indispensable for prophase I arrest and to function at the G2/M checkpoint.[13]

CHEK2 regulates cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development.[14] Although CHEK2 is a down stream effector of the ATM kinase that responds primarily to double-strand breaks it can also be activated by ATR (ataxia-telangiectasia and Rad3 related) kinase that responds primarily to single-strand breaks. In mouse, CHEK2 is essential for DNA damage surveillance in female meiosis. The response of oocytes to DNA double-strand break damage involves a pathway hierarchy in which ATR kinase signals to CHEK2 which then activates p53 and p63 proteins.[15]

In the fruitfly Drosophila, irradiation of germ line cells generates double-strand breaks that result in cell cycle arrest and apoptosis. The Drosophila CHEK2 ortholog mnk and the p53 ortholog dp53 are required for much of the cell death observed in early oogenesis when oocyte selection and meiotic recombination occur.[16]

Meiosis-specific Transcription factor Ndt80

Ndt80 is a meiosis-specific transcription factor required for successful completion of meiosis and spore formation.[17] The protein recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation.[17][18][19] The DNA-binding domain of Ndt80 has been isolated, and the structure reveals that this protein is a member of the Ig-fold family of transcription factors.[20] Ndt80 also competes with the repressor SUM1 for binding to promoters containing MSEs.[21]

Transitions in yeast

When a mutation inactivates Ndt80 in budding yeast, meiotic cells display a prolonged delay in late pachytene, the third stage of prophase.[22] The cells display intact synaptonemal complexes but eventually arrest in the diffuse chromatin stage that follows pachytene. This checkpoint-mediated arrest prevents later events from occurring until earlier events have been executed successfully and prevents chromosome missegregation.[23][24]

Role in cell cycle progression

NDt80 is crucial for the completion of prophase and entry into meiosis 1, as it stimulates the expression of a large number of middle meiotic genes. Ndt80 is regulated through transcriptional and post-translational mechanisms (i.e. phosphorylation).

Interaction with Clb1

Ndt80 stimulates the expression of the B-type cyclin Clb-1, which greatly interacts with Cdk1 during meiotic divisions.[25] Active complexes of Clb-1 with Cdk1 play a large role in triggering the events of the first meiotic division, and their activity is restricted to meiosis 1.[26]

Interaction with Ime2

Ndt80 stimulates expression of itself and expression of protein kinase Ime2, both of which feedback to further stimulate Ndt80. This increased amount of Ndt80 protein further enhances the transcription of target genes.[24] Early in meiosis 1, Ime2 activity rises and is required for the normal accumulation and activity of Ndt80. However, if Ndt80 is expressed prematurely, it will initially accumulate in an unmodified form. Ime2 can then also act as a meiosis-specific kinase that phosphorylates Ndt80, resulting in fully activated Ndt80.[27]

Expression of Plk

Ndt80 stimulates the expression of the gene that encodes polo-like kinase, Plk. This protein is activated in late pachytene and is needed for crossover formation and partial loss of cohesion from chromosome arms. Plk is also both necessary and sufficient to trigger exit from pachytene points.[28][29]

Recombination model

The meiotic recombination checkpoint operates in response to defects in meiotic recombination and chromosome synapsis, potentially arresting cells before entry into meiotic divisions.[30] Because recombination is initiated by double stranded breaks (DSBs) at certain regions of the genome, entry into Meiosis 1 must be delayed until the DSBs are repaired.[31] The meiosis-specific kinase Mek1 plays an important role in this and recently, it has been discovered that Mek1 is able to phosphorylate Ndt80 independently of IME2. This phosphorylation, however, is inhibitory and prevents Ndt80 from binding to MSEs in the presence of DSBs.[32]

Heterokaryon Incompatibility

Heterokaryon Incompatibility (HI) has been likened to a fungal immune system;[33] it is a non-self recognition mechanism that is ubiquitous among filamentous members of the Asomycota phylum of the Fungi kingdom.[34] Vib-1 is an Ndt80 homologue in Neurospora crassa and is required for HI in this species. It has been found that mutations at the vib1 locus suppress non-self recognition, and VIB-1 is required for the production of downstream effectors associated with HI, such as extracellular proteases.[35][36]

Female sexual development

Studies have indicated that Ndt80 homologues also play a role in female sexual development in fungi species other than the more commonly studied Saccharomyces cerevisiae.[35][37] Mutations in vib-1 have been found to affect the timing and development of female reproductive structures prior to fertilization.[37]

Role in Cancer

Although usually characterized in yeast and other fungi, the DNA-binding domain of Ndt80 is homologous to a number of proteins in higher eukaryotes and the residues used for binding are highly conserved. In humans, the Ndt80 homologue C11orf9 is highly expressed in invasive or metastatic tumor cells, suggesting potential usage as a target molecule in cancer treatment.[38] However, not much progress has been made on this front in recent years.

See also

References
  1. Morgan DO (2007). "Chapter 9: Meitosis". The Cell Cycle: Principles of Control. London: New Science Press Ltd. ISBN 978-0-87893-508-6.
  2. Malik SB, Pightling AW, Stefaniak LM, Schurko AM, Logsdon JM (August 2007). Hahn MW (ed.). "An expanded inventory of conserved meiotic genes provides evidence for sex in Trichomonas vaginalis". PLOS ONE. 3 (8): e2879. Bibcode:2008PLoSO...3.2879M. doi:10.1371/journal.pone.0002879. PMC 2488364. PMID 18663385.
  3. Barchi M, Mahadevaiah S, Di Giacomo M, Baudat F, de Rooij DG, Burgoyne PS, et al. (August 2005). "Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage" (PDF). Molecular and Cellular Biology. 25 (16): 7203–15. doi:10.1128/MCB.25.16.7203-7215.2005. PMC 1190256. PMID 16055729.
  4. Storlazzi A, Tessé S, Gargano S, James F, Kleckner N, Zickler D (November 2003). "Meiotic double-strand breaks at the interface of chromosome movement, chromosome remodeling, and reductional division". Genes & Development. 17 (21): 2675–87. doi:10.1101/gad.275203. PMC 280617. PMID 14563680.
  5. Bhalla N, Dernburg AF (December 2005). "A conserved checkpoint monitors meiotic chromosome synapsis in Caenorhabditis elegans". Science. 310 (5754): 1683–6. Bibcode:2005Sci...310.1683B. doi:10.1126/science.1117468. PMID 16339446.
  6. Odorisio T, Rodriguez TA, Evans EP, Clarke AR, Burgoyne PS (March 1998). "The meiotic checkpoint monitoring synapsis eliminates spermatocytes via p53-independent apoptosis". Nature Genetics. 18 (3): 257–61. doi:10.1038/ng0398-257. PMID 9500548.
  7. Turner JM, Mahadevaiah SK, Elliott DJ, Garchon HJ, Pehrson JR, Jaenisch R, Burgoyne PS (November 2002). "Meiotic sex chromosome inactivation in male mice with targeted disruptions of Xist". Journal of Cell Science. 115 (Pt 21): 4097–105. doi:10.1242/jcs.00111. PMID 12356914.
  8. Di Giacomo M, Barchi M, Baudat F, Edelmann W, Keeney S, Jasin M (January 2005). "Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants". Proceedings of the National Academy of Sciences of the United States of America. 102 (3): 737–42. Bibcode:2005PNAS..102..737D. doi:10.1073/pnas.0406212102. PMC 545532. PMID 15640358.
  9. Manterola M, Page J, Vasco C, Berríos S, Parra MT, Viera A, et al. (August 2009). Hawley RS (ed.). "A high incidence of meiotic silencing of unsynapsed chromatin is not associated with substantial pachytene loss in heterozygous male mice carrying multiple simple robertsonian translocations". PLoS Genetics. 5 (8): e1000625. doi:10.1371/journal.pgen.1000625. PMC 2726437. PMID 19714216.
  10. MacQueen AJ, Hochwagen A (July 2011). "Checkpoint mechanisms: the puppet masters of meiotic prophase". Trends in Cell Biology. 21 (7): 393–400. doi:10.1016/j.tcb.2011.03.004. PMID 21531561.
  11. Subramanian VV, Hochwagen A (October 2014). "The meiotic checkpoint network: step-by-step through meiotic prophase". Cold Spring Harbor Perspectives in Biology. 6 (10): a016675. doi:10.1101/cshperspect.a016675. PMC 4176010. PMID 25274702.
  12. Flaggs G, Plug AW, Dunks KM, Mundt KE, Ford JC, Quiggle MR, et al. (December 1997). "Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes". Current Biology. 7 (12): 977–86. doi:10.1016/s0960-9822(06)00417-9. PMID 9382850.
  13. Chen L, Chao SB, Wang ZB, Qi ST, Zhu XL, Yang SW, et al. (May 2012). "Checkpoint kinase 1 is essential for meiotic cell cycle regulation in mouse oocytes". Cell Cycle. 11 (10): 1948–55. doi:10.4161/cc.20279. PMID 22544319.
  14. Dai XX, Duan X, Liu HL, Cui XS, Kim NH, Sun SC (February 2014). "Chk2 regulates cell cycle progression during mouse oocyte maturation and early embryo development". Molecules and Cells. 37 (2): 126–32. doi:10.14348/molcells.2014.2259. PMC 3935625. PMID 24598997.
  15. Bolcun-Filas E, Rinaldi VD, White ME, Schimenti JC (January 2014). "Reversal of female infertility by Chk2 ablation reveals the oocyte DNA damage checkpoint pathway". Science. 343 (6170): 533–6. Bibcode:2014Sci...343..533B. doi:10.1126/science.1247671. PMC 4048839. PMID 24482479.
  16. Shim HJ, Lee EM, Nguyen LD, Shim J, Song YH (2014). "High-dose irradiation induces cell cycle arrest, apoptosis, and developmental defects during Drosophila oogenesis". PLOS ONE. 9 (2): e89009. Bibcode:2014PLoSO...989009S. doi:10.1371/journal.pone.0089009. PMC 3923870. PMID 24551207.
  17. Xu L, Ajimura M, Padmore R, Klein C, Kleckner N (December 1995). "NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae". Molecular and Cellular Biology. 15 (12): 6572–81. doi:10.1128/MCB.15.12.6572. PMC 230910. PMID 8524222.
  18. Chu S, Herskowitz I (April 1998). "Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80". Molecular Cell. 1 (5): 685–96. doi:10.1016/S1097-2765(00)80068-4. PMID 9660952.
  19. Ozsarac N, Straffon MJ, Dalton HE, Dawes IW (March 1997). "Regulation of gene expression during meiosis in Saccharomyces cerevisiae: SPR3 is controlled by both ABFI and a new sporulation control element". Molecular and Cellular Biology. 17 (3): 1152–9. doi:10.1128/MCB.17.3.1152. PMC 231840. PMID 9032242.
  20. Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN (November 2002). "Structure of the sporulation-specific transcription factor Ndt80 bound to DNA". The EMBO Journal. 21 (21): 5721–32. doi:10.1093/emboj/cdf572. PMC 131069. PMID 12411490.
  21. Hendrickson WA, Ward KB (October 1975). "Atomic models for the polypeptide backbones of myohemerythrin and hemerythrin". Biochemical and Biophysical Research Communications. 66 (4): 1349–56. doi:10.1016/0006-291x(75)90508-2. PMID 5.
  22. Morgan DO (2007). The Cell Cycle: Principles of Control. New Science Press Ltd. p. 186.
  23. Roeder GS, Bailis JM (September 2000). "The pachytene checkpoint". Trends in Genetics. 16 (9): 395–403. doi:10.1016/s0168-9525(00)02080-1. PMID 10973068.
  24. Tung KS, Hong EJ, Roeder GS (October 2000). "The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80". Proceedings of the National Academy of Sciences of the United States of America. 97 (22): 12187–92. Bibcode:2000PNAS...9712187T. doi:10.1073/pnas.220464597. PMC 17316. PMID 11035815.
  25. Tibbles KL, Sarkar S, Novak B, Arumugam P (2013-11-01). Wang Y (ed.). "CDK-dependent nuclear localization of B-cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast". PLOS ONE. 8 (11): e79001. Bibcode:2013PLoSO...879001T. doi:10.1371/journal.pone.0079001. PMC 3815228. PMID 24223874.
  26. Carlile TM, Amon A (April 2008). "Meiosis I is established through division-specific translational control of a cyclin". Cell. 133 (2): 280–91. doi:10.1016/j.cell.2008.02.032. PMC 2396536. PMID 18423199.
  27. Sopko R, Raithatha S, Stuart D (October 2002). "Phosphorylation and maximal activity of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is dependent on Ime2". Molecular and Cellular Biology. 22 (20): 7024–40. doi:10.1128/MCB.22.20.7024-7040.2002. PMC 139797. PMID 12242283.
  28. Clyne RK, Katis VL, Jessop L, Benjamin KR, Herskowitz I, Lichten M, Nasmyth K (May 2003). "Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I". Nature Cell Biology. 5 (5): 480–5. doi:10.1038/ncb977. PMID 12717442.
  29. Sourirajan A, Lichten M (October 2008). "Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis". Genes & Development. 22 (19): 2627–32. doi:10.1101/gad.1711408. PMC 2559907. PMID 18832066.
  30. Pak J, Segall J (September 2002). "Role of Ndt80, Sum1, and Swe1 as targets of the meiotic recombination checkpoint that control exit from pachytene and spore formation in Saccharomyces cerevisiae". Molecular and Cellular Biology. 22 (18): 6430–40. doi:10.1128/MCB.22.18.6430-6440.2002. PMC 135635. PMID 12192042.
  31. Keeney S, Lange J, Mohibullah N (2014-11-23). "Self-organization of meiotic recombination initiation: general principles and molecular pathways". Annual Review of Genetics. 48 (1): 187–214. doi:10.1146/annurev-genet-120213-092304. PMC 4291115. PMID 25421598.
  32. Chen X, Gaglione R, Leong T, Bednor L, de Los Santos T, Luk E, et al. (November 2018). Lichten M (ed.). "Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80". PLoS Genetics. 14 (11): e1007832. doi:10.1371/journal.pgen.1007832. PMC 6289461. PMID 30496175.
  33. Paoletti M, Saupe SJ (November 2009). "Fungal incompatibility: evolutionary origin in pathogen defense?". BioEssays. 31 (11): 1201–10. doi:10.1002/bies.200900085. PMID 19795412.
  34. Saupe SJ (September 2000). "Molecular genetics of heterokaryon incompatibility in filamentous ascomycetes". Microbiology and Molecular Biology Reviews. 64 (3): 489–502. doi:10.1128/MMBR.64.3.489-502.2000. PMC 99001. PMID 10974123.
  35. Hutchison EA, Glass NL (August 2010). "Meiotic regulators Ndt80 and ime2 have different roles in Saccharomyces and Neurospora". Genetics. 185 (4): 1271–82. doi:10.1534/genetics.110.117184. PMC 2927755. PMID 20519745.
  36. Dementhon K, Iyer G, Glass NL (December 2006). "VIB-1 is required for expression of genes necessary for programmed cell death in Neurospora crassa". Eukaryotic Cell. 5 (12): 2161–73. doi:10.1128/EC.00253-06. PMC 1694810. PMID 17012538.
  37. Katz ME, Cooper S (October 2015). "Extreme Diversity in the Regulation of Ndt80-Like Transcription Factors in Fungi". G3. 5 (12): 2783–92. doi:10.1534/g3.115.021378. PMC 4683649. PMID 26497142.
  38. Montano SP, Pierce M, Coté ML, Vershon AK, Georgiadis MM (December 2002). "Crystallographic studies of a novel DNA-binding domain from the yeast transcriptional activator Ndt80". Acta Crystallographica. Section D, Biological Crystallography. 58 (Pt 12): 2127–30. doi:10.2210/pdb1m6u/pdb. PMID 12454476.
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