OK cells

OK cells (short for opossum kidney cells) are a marsupial cell line used in medical research to model proximal tubule epithelial cells of the kidney.

Characteristics

The cell line was derived from the kidney of an adult female North American opossum (Didelphis virginiana).[1] Like porcine LLC-PK1 cells, this cell line has the limitation of lacking several enzymes specific to the proximal tubule.[2] Nonetheless, OK cells have been used extensively to study functional interactions between the parathyroid hormone 1 receptor (PTH1R) and the sodium-hydrogen exchange regulatory factor 1 (NHERF1).[3]

Use in research

OK cells were originally cultured as a source of X chromosomes for studies on X inactivation.[1] They have also served as models for the study of renal dopaminergic physiology, owing to their capacity to produce and degrade dopamine.[4] OK cells are sold under the catalog number CRL-1840 by ATCC.

References
  1. Koyama, H.; Goodpasture, C.; Miller, M. M.; Teplitz, R. L.; Riggs, A. D. (March 1978). "Establishment and characterization of a cell line from the American opossum (Didelphys virginiana)". In Vitro. 14 (3): 239–246. doi:10.1007/BF02616032. PMID 566717. S2CID 27331795.
  2. Kruidering, Marieke; Water, Bob van der; Nagelkerke, J. Fred (1996). Methods for Studying Renal Toxicity. Archives of Toxicology. 18. pp. 173–83. doi:10.1007/978-3-642-61105-6. ISBN 978-3-642-64696-6. PMID 8678793. S2CID 27034550.
  3. Ciruela, Francisco; Luján, Rafael (2008). Molecular Aspects of G Protein-Coupled Receptors: Interacting Proteins and Function. New York, NY: Nova Science Publishers. p. 219. ISBN 9781600219153.
  4. Guimarães, J.T.; et al. (April 1997). "Opossum kidney (OK) cells in culture synthesize and degrade the natriuretic hormone dopamine: A comparison with rat renal tubular cells". The International Journal of Biochemistry & Cell Biology. 29 (4): 681–688. doi:10.1016/S1357-2725(96)00166-5. PMID 9363646.
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