Polyamine oxidase
In enzymology, a polyamine oxidase (EC 1.5.3.11) is an enzyme that catalyzes the chemical reaction
- N1-acetylspermine + O2 + H2O N1-acetylspermidine + 3-aminopropanal + H2O2
| polyamine oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.5.3.11 | ||||||||
| CAS number | 294646-71-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 3 substrates of this enzyme are N1-acetylspermine, O2, and H2O, whereas its 3 products are N1-acetylspermidine, 3-aminopropanal, and H2O2.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is N1-acetylspermidine:oxygen oxidoreductase (deaminating). This enzyme is also called 1-N-acetylspermidine:oxygen oxidoreductase (deaminating). It has 2 cofactors: FAD, and Iron. A similar enzyme in plants catalyzes non-acetylated polyamines.[1]
Structural studies
As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes 1B37, 1H81, 1H82, 1H83, 1H84, 1H86, 1RSG, 1YY5, and 1Z6L.
References
- Moschou, Panagiotis; Maite Sanmartin; Athina H Andriopoulou; Enrique Rojo; Jose J Sanchez-Serrano; Kalliopi A Roubelakis-Angelakis (1 August 2008). "Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in Arabidopsis". Plant Physiology. 147 (4): 1845–1857. doi:10.1104/pp.108.123802. PMC 2492618. PMID 18583528.
- Bolkenius FN, Seiler N (1981). "Acetylderivatives as intermediates in polyamine catabolism". Int. J. Biochem. 13 (3): 287–292. doi:10.1016/0020-711X(81)90080-X. PMID 7215618.
- Holtta E (1983). Polyamine oxidase (rat liver). Methods Enzymol. Methods in Enzymology. 94. pp. 306–311. doi:10.1016/S0076-6879(83)94054-5. ISBN 978-0-12-181994-1. PMID 6621391.