Sørensen formol titration

The Sørensen formol titration(SFT) invented by S. P. L. Sørensen in 1907 [1] is a titration of an amino acid with potassium hydroxide in the presence of formaldehyde.[2] It is used in the determination of protein content in samples.[3]

Formol titration equation for amino acids in general

If instead of an amino acid an ammonium salt is used the reaction product with formaldehyde is hexamethylenetetramine:

The liberated hydrochloric acid is then titrated with the base and the amount of ammonium salt used can be determined.

With an amino acid the formaldehyde reacts with the amino group to form a methylene amino (R-N=CH2) group. The remaining acidic carboxylic acid group can then again be titrated with base.[3]

In winemaking

Formol titration is one of the methods used in winemaking to measure yeast assimilable nitrogen needed by wine yeast in order to successfully complete fermentation.[4]

Accuracy in formol titration
Formol titration equation for proline

There has been some inaccuracies of the SFT caused by the differences in the basicity of the nitrogen in different amino acids which were explained by S. L. Jodidi. For instances, proline(an imino acid), histidine, and lysine yields too low values compared to the theory. Unlike alpha, monobasic (containing one amino group per molecule) amino acids, these amino (or imino) acids' nitrogens have inconstant basicity, which results in partial reaction with formaldehyde.[5]

In case of tyrosine, the actual results are too high due to the negative hydroxyl group (-OH), which acts as a base. This explanation is supported by the fact that phenylalanine can be accurately titrated.[5]

Formol titration equation for secondary amines
Formol titration equation for histidine

References
  1. Sørensen Biochem Z., 7, 45, 407 1907
  2. Harry Auterhoff: Lehrbuch der Pharmazeutischen Chemie, 5. Aufl., Wissenschaftliche Verlagsgesellschaft mbH Stuttgart, 1968.
  3. Analytical Chemistry of Foods C.S. James Springer Science & Business Media, 2013
  4. B. Zoecklein, K. Fugelsang, B. Gump, F. Nury Wine Analysis and Production pgs 152–163, 340–343, 444–445, 467 Kluwer Academic Publishers, New York (1999) ISBN 0834217015
  5. S. L. Jodidi "Abnormalities In the Formol Titration Method" Journal of American Chemical Society 1918 40 (7), 1031-1035 DOI: 10.1021/ja02240a006


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