SERINC3

Serine incorporator 3 is a protein that in humans is encoded by the SERINC3 gene.[5][6] It has been demonstrated that SERINC3 acts as a retrovirus restriction factor. [7][8][9]

SERINC3
Identifiers
Aliases	SERINC3, AIGP1, DIFF33, SBBI99, TDE, TDE1, TMS-1, serine incorporator 3
External IDs	OMIM: 607165 MGI: 1349457 HomoloGene: 38230 GeneCards: SERINC3
Gene location (Human) Chr. Chromosome 20 (human)[1] Band 20q13.12 Start 44,496,221 bp[1] End 44,522,085 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 H3 Start 163,623,272 bp[2] End 163,645,131 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • L-serine transmembrane transporter activity Cellular component • integral component of membrane • Golgi membrane • Golgi apparatus • membrane • cytoplasm • perinuclear region of cytoplasm • cell membrane Biological process • phosphatidylserine metabolic process • detection of virus • innate immune system • defense response to virus • positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway • sphingolipid metabolic process • L-serine transport • immune system process • L-serine biosynthetic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10955	26943
Ensembl	ENSG00000132824	ENSMUSG00000017707
UniProt	Q13530	Q9QZI9
RefSeq (mRNA)	NM_198941 NM_006811	NM_012032
RefSeq (protein)	NP_006802 NP_945179	NP_036162
Location (UCSC)	Chr 20: 44.5 – 44.52 Mb	Chr 2: 163.62 – 163.65 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Model organisms have been used in the study of SERINC3 function. A conditional knockout mouse line called Serinc3^{tm1a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.[10] Male and female animals underwent a standardized phenotypic screen[11] to determine the effects of deletion.[12][13][14][15] Additional screens performed: - In-depth immunological phenotyping[16]

Serinc3 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[11][16]
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Abnormal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. GRCh38: Ensembl release 89: ENSG00000132824 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000017707 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bossolasco M, Lebel M, Lemieux N, Mes-Masson AM (November 1999). "The human TDE gene homologue: localization to 20q13.1-13.3 and variable expression in human tumor cell lines and tissue". Molecular Carcinogenesis. 26 (3): 189–200. doi:10.1002/(SICI)1098-2744(199911)26:3<189::AID-MC8>3.0.CO;2-T. PMID 10559794.
6. "Entrez Gene: SERINC3 serine incorporator 3".
7. Rosa A, Chande A, Ziglio S, De Sanctis V, Bertorelli R, Goh SL, et al. (October 2015). "HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation". Nature. 526 (7572): 212–7. doi:10.1038/nature15399. PMC 4861059. PMID 26416734.
8. Chande A, Cuccurullo EC, Rosa A, Ziglio S, Carpenter S, Pizzato M (November 2016). "S2 from equine infectious anemia virus is an infectivity factor which counteracts the retroviral inhibitors SERINC5 and SERINC3". Proceedings of the National Academy of Sciences of the United States of America. 113 (46): 13197–13202. doi:10.1073/pnas.1612044113. PMC 5135340. PMID 27803322.
9. Ramdas P, Bhardwaj V, Singh A, Vijay N, Chande A (2020-02-24). "Coevolution of retroviruses with SERINCs following whole-genome duplication divergence". bioRxiv. doi:10.1101/2020.02.24.962506.
10. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
11. "International Mouse Phenotyping Consortium".
12. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, et al. (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
13. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
14. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
15. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, et al. (July 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
16. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, et al. (September 1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). Nature. 377 (6547 Suppl): 3–174. PMID 7566098.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Bonaldo MF, Lennon G, Soares MB (September 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Bossolasco M, Veillette F, Bertrand R, Mes-Masson AM (August 2006). "Human TDE1, a TDE1/TMS family member, inhibits apoptosis in vitro and stimulates in vivo tumorigenesis". Oncogene. 25 (33): 4549–58. doi:10.1038/sj.onc.1209488. PMID 16547497.