STAP1

Signal-transducing adaptor protein 1 is a protein that in humans is encoded by the STAP1 gene.[5][6][7]

STAP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1X1F, 3MAZ
Identifiers
Aliases	STAP1, BRDG1, STAP-1, signal transducing adaptor family member 1
External IDs	OMIM: 604298 MGI: 1926193 HomoloGene: 8103 GeneCards: STAP1
Gene location (Human) Chr. Chromosome 4 (human)[1] Band 4q13.2 Start 67,558,727 bp[1] End 67,607,337 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 E1 Start 86,071,746 bp[2] End 86,106,125 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • macrophage colony-stimulating factor receptor binding • transmembrane receptor protein tyrosine kinase adaptor activity • phosphotyrosine residue binding • receptor tyrosine kinase binding • GO:0001948 protein binding • phospholipid binding • protein kinase binding • signaling adaptor activity Cellular component • cytoplasm • mitochondrion • cell nucleus • nucleoplasm • intracellular membrane-bounded organelle • macromolecular complex Biological process • positive regulation of B cell receptor signaling pathway • positive regulation of phagocytosis, engulfment • transmembrane receptor protein tyrosine kinase signaling pathway • negative regulation of ruffle assembly • positive regulation of microglial cell mediated cytotoxicity • positive regulation of non-membrane spanning protein tyrosine kinase activity • negative regulation of microglial cell migration • positive regulation of gene expression • positive regulation of microglial cell activation • negative regulation of phosphorylation • cellular response to lipopolysaccharide • negative regulation of macrophage colony-stimulating factor signaling pathway • positive regulation of actin cytoskeleton reorganization • negative regulation of macrophage chemotaxis • response to bacterium Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	26228	56792
Ensembl	ENSG00000035720	ENSMUSG00000029254
UniProt	Q9ULZ2	Q9JM90
RefSeq (mRNA)	NM_012108 NM_001317769	NM_019992 NM_001310639
RefSeq (protein)	NP_001304698 NP_036240	NP_001297568 NP_064376
Location (UCSC)	Chr 4: 67.56 – 67.61 Mb	Chr 5: 86.07 – 86.11 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. A mouse ortholog, stem cell adaptor protein 1, shares 83% identity with its human counterpart.[7]

Interactions

STAP1 has been shown to interact with C19orf2.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000035720 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029254 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ohya K, Kajigaya S, Kitanaka A, Yoshida K, Miyazato A, Yamashita Y, Yamanaka T, Ikeda U, Shimada K, Ozawa K, Mano H (Nov 1999). "Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase". Proc Natl Acad Sci U S A. 96 (21): 11976–81. doi:10.1073/pnas.96.21.11976. PMC 18397. PMID 10518561.
6. Masuhara M, Nagao K, Nishikawa M, Sasaki M, Yoshimura A, Osawa M (Mar 2000). "Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains". Biochem Biophys Res Commun. 268 (3): 697–703. doi:10.1006/bbrc.2000.2223. PMID 10679268.
7. "Entrez Gene: BRDG1 BCR downstream signaling 1".
8. Gstaiger M, Luke B, Hess D, Oakeley EJ, Wirbelauer C, Blondel M, Vigneron M, Peter M, Krek W (Nov 2003). "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI". Science. 302 (5648): 1208–12. doi:10.1126/science.1088401. PMID 14615539. S2CID 26836888.

Further reading

• Kurosaki T (1999). "Genetic analysis of B cell antigen receptor signaling". Annu. Rev. Immunol. 17 (1): 555–92. doi:10.1146/annurev.immunol.17.1.555. PMID 10358768.
• Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M (2001). "Creation of genome-wide protein expression libraries using random activation of gene expression". Nat. Biotechnol. 19 (5): 440–5. doi:10.1038/88107. PMID 11329013. S2CID 25064683.
• Yokohari K, Yamashita Y, Okada S, Ohya K, Oda S, Hatano M, Mano H, Hirasawa H, Tokuhisa T (2002). "Isoform-dependent interaction of BRDG1 with Tec kinase". Biochem. Biophys. Res. Commun. 289 (2): 414–20. doi:10.1006/bbrc.2001.6008. PMID 11716489.
• Minoguchi M, Minoguchi S, Aki D, Joo A, Yamamoto T, Yumioka T, Matsuda T, Yoshimura A (2003). "STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif". J. Biol. Chem. 278 (13): 11182–9. doi:10.1074/jbc.M211230200. PMID 12540842.
• Ma J, Dempsey AA, Stamatiou D, Marshall KW, Liew CC (2007). "Identifying leukocyte gene expression patterns associated with plasma lipid levels in human subjects". Atherosclerosis. 191 (1): 63–72. doi:10.1016/j.atherosclerosis.2006.05.032. PMID 16806233.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.