TMEM165

Transmembrane protein 165 is a protein that in humans is encoded by the TMEM165 gene.[1] The gene is also known as TPARL and TMPT27.[1]

Model organisms

Tmem165 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Abnormal[2]
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Salmonella infection	Abnormal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of TMEM165 function. A conditional knockout mouse line, called Tmem165^{tm1e(EUCOMM)Wtsi}[7] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[8][9][10]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][11] Twenty three tests were carried out on mutant mice and two significant abnormalities were observed.[5] Homozygous mutant males had skeletal deformities, including scoliosis, and an increased susceptibility to bacterial infection.[5]

References

1. "Transmembrane protein 165". Retrieved 2011-12-06.
2. "Radiography data for Tmem165". Wellcome Trust Sanger Institute.
3. "Salmonella infection data for Tmem165". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Tmem165". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
9. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
10. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
11. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Hu, R. -M.; Han, Z. G.; Song, H. D.; Peng, Y. D.; Huang, Q. H.; Ren, S. X.; Gu, Y. J.; Huang, C. H.; Li, Y. B.; Jiang, C. -L.; Fu, G.; Zhang, Q. -H.; Gu, B. -W.; Dai, M.; Mao, Y. -F.; Gao, G. -F.; Rong, R.; Ye, M.; Zhou, J.; Xu, S. -H.; Gu, J.; Shi, J. -X.; Jin, W. -R.; Zhang, C. -K.; Wu, T. -M.; Huang, G. -Y.; Chen, Z.; Chen, M. -D.; Chen, J. -L. (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proceedings of the National Academy of Sciences. 97 (17): 9543–9548. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.