TMEM242

Transmembrane protein 242 (TMEM242) is a protein that in humans is encoded by the TMEM242 gene.[5] The tmem242 gene is located on chromosome 6, on the long arm, in band 2 section 5.3. This protein is also commonly called C6orf35, BM033, and UPF0463 Transmembrane Protein C6orf35. The tmem242 gene is 35,238 base pairs long, and the protein is 141 amino acids in length. The tmem242 gene contains 4 exons.[6] The function of this protein is not well understood by the scientific community. This protein contains a DUF1358 domain (Domain of Unknown Function 1358).[7]

TMEM242
Identifiers
AliasesTMEM242, BM033, C6orf35, transmembrane protein 242
External IDsMGI: 1917794 HomoloGene: 44020 GeneCards: TMEM242
Gene location (Human)
Chr.Chromosome 6 (human)[1]
Band6q25.3Start157,289,025 bp[1]
End157,323,601 bp[1]
Orthologs
SpeciesHumanMouse
Entrez

729515

70544

Ensembl

ENSG00000215712

ENSMUSG00000004945

UniProt

Q9NWH2

Q8VCR3

RefSeq (mRNA)

NM_018452

NM_027457

RefSeq (protein)

NP_060922

NP_081733

Location (UCSC)Chr 6: 157.29 – 157.32 MbChr 17: 5.41 – 5.44 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Domain

The TMEM242 protein has a conserved domain of unknown function pfam 07096, DUF 1358., which covers the first 121 aa of the protein. This domain is conserved in eukaryotes.

Associated Proteins

Several predicted interacting proteins and functional sites on the protein have been identified. One of the predicted interacting protein is MAP2K1IP1, which is a scaffold protein.[8] This protein is known to be involved in the MAP Kinase pathway. The MAP Kinase pathway is associated with the Alzheimer's pathway through a protein called Tau or MAPT. Excessive phosphorylation of this protein leads to aggregation of neurons which can cause Alzheimer's disease. Other associated proteins include GGT7,[9][10] RNF5,[9][10] ELOVL4,[9] GPR42,[9] BCL2L13,[9] HEATR1,[11] IZUMO4,[11] ARID1B,[11] SIAE,[11] EDARADD,[11] URB1,[11] ZDHHC14,[11] KIF11,[11] RRM2,[11] KCTD13,[11] TMEM31,[10] NDUFA3,[10] SGPL1,[10] CNR2,[10] and GJA8.[10] All of the listed proteins have known functions.

Function

The tmem242 protein is highly expressed in many tissues, but the most highly expressed in the brain, heart, adrenal, and thyroid.[12]

Homologs and orthologs

There are homologs and orthologs of the tmem242 protein in a variety of species. The species have diverged as far back as 794 million years ago.[13] The following species have been found as having orthologs to the tmem242 protein in their genome:[14]

  • TMEM242, H. sapiens
  • TMEM242, P. troglodytes
  • Tmem242, M. musculus
  • C1H6orf35, R. norvegicus
  • TMEM242, C. lupus
  • TMEM242, B. taurus
  • C3H6ORF35, G. gallus
  • tmem242, D. rerio
  • tmem242, X. tropicalis
  • AgaP_AGAP009165, A. gambiae
  • CG11699, D. melanogaster


This image shows the corrected divergence for tmem242 compared to fibrinogen and cytochrome C

The image to the right represents the rate of divergence for tmem242 (blue) compared to fibrinogen (red) and cytochrome C (green). This graph shows that tmem242 is evolving at a rate that is in between that of fibrinogen, which evolves quickly, and cytochrome C, which evolves slowly.

Gene Level Regulation

The promoter for the tmem242 gene is located upstream, but includes the transcription and translational start sites. This promoter was found using ElDorado by Genomatix.[15] This promoter has many transcription factor binding sites. Some of these transcription factors include TFIIB, Mouse Krueppel like factor, and NKX homeodomain factors. One notable transcription factor is the Retinoblastoma-binding protein with demethylase activity. There are ubiquitous basal level expression of tmem242 in all tissues in human[6] and mouse.[16] There are other tissues with increase expression levels such as the cerebellum and the hippocampus. There is increase expression levels in many parts of the brain along with kidney and prostate.[17] Tmem242 is also expressed in smaller amounts such as heart, adrenal gland, and thyroid.[6]

Transcriptional Level Regulation

Tmem242 has several predicted post-transcriptional stem loop structures in the 5' UTR.[18] These structures use binding, both traditional and modified, to create stem loop structures in the untranslated regions of the mRNA.

Protein Level Regulation

There are various forms of protein level regulation for tmem242. Tmem242 is found in a membrane within the cell. It is likely tmem242 is found in the cellular membrane[19] or the mitochondrial membrane,[20] but other sub cellular locations are possible. These include the endoplasmic reticulum and the nuclear membrane. There are various phosphorylation sites present on tmem242. These sites include cAMP and cGMP dependent protein kinase phosphorylation sites, casein kinase II phosphorylation sites, and a protein kinase C phosphorylation site.[21] These site all favor the phosphorylation of serine and threonine residues.[22] Other post-translational modifications include a N-myristoylation site, which covalently adds a myristate molecule.[22]

Protein Composition

There are various single nucleotide polymorphisms (SNPs) that can occur in tmem242. A few cause stop codons, others make either silent mutations or missense mutations.

Tmem242 is 141 amino acids long, and contains at least one residue of each amino acid. There are two transmembrane domains, one spanning from approximately the 28th residue to the 48th reside. The second domain spans from approximately the 82nd residue to the 102nd residue.[23] These amino acid residues are approximate as various sources have approximations for the transmembrane domains.

Secondary Structure

Tmem242 has several secondary structures. The two transmembrane domains have alpha helices.[24] These helices are formed from uncharged residues that are buried in the membrane. These helices are not exposed for binding. Other parts of the tmem242 protein may form secondary structures.

Tertiary Structure

The tmem242 protein further folds to its final structure to embed in a membrane. It is likely tmem242 is embedded in the cellular membrane,[25] there is also potential for tmem242 to embed in the mitochondrial membrane or the endoplasmic reticulum membrane.

Post-translational Modification

Tmem242 undergoes various post-translational modification. There are 11 serine residues that have a potential to be phosphorylated. Those sites occur in residue numbers 13, 20, 51, 57, 65, 74, 76, 77, 119, 128, and 130.[26] There are also four threonine residues that have the potential to be phosphorylated. Those site are residue numbers 36, 49, 123, and 137.[26] Other post-translational modifications that potentially happen occur at specific motif sites. There are 12 motif sites in tmem242 that correlate to 4 different motif types. The four types are cAMP and cGMP dependent protein kinase phosphorylation site, casein kinase II phosphorylation site, N-myristoylation site, and protein kinase C phosphorylation site.[22] There is one cAMP and cGMP dependent protein kinase phosphorylation site in tmem242, and this motif has a preference for phosphorylation of threonine and serine residues.[22] There are four casein kinase II phosphorylation sites, and this motif is a serine/threonine kinase. There are six N-myristoylation sites in tmem242, and this motif signals a covalent attachment of myristate.[22] There is one protein kinase C phosphorylation site which signals phosphorylation of serine or threonine resides.[22]

References
  1. GRCh38: Ensembl release 89: ENSG00000215712 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000004945 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Entrez Gene: chromosome 6 open reading frame 35".
  6. "transmembrane protein 242 [Homo sapiens]". Protein - NCBI. National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 2019-02-26.
  7. "Pfam Family: DUF1358 (PF07096)". Pfam.
  8. "C6orf35 protein (Homo sapiens)". STRING interaction network.
  9. Calderone A. "TMEM242". Mentha: the intercome browser.
  10. "48 binary interactions found for search term tmem242". IntAct Molecular Interaction Database. EMBL-EBI.
  11. "STRING: functional protein association networks". string-db.org. Retrieved 2019-04-22.
  12. "TMEM242 transmembrane protein 242 [Homo sapiens (human)]". Gene - NCBI. National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 2019-02-26.
  13. "TimeTree :: The Timescale of Life". timetree.org. Retrieved 2019-03-04.
  14. "HomoloGene:44020". HomoloGene. National Center for Biotechnology Information, U.S. National Library of Medicine.
  15. "Genomatix - NGS Data Analysis & Personalized Medicine". www.genomatix.de. Retrieved 2019-05-15.
  16. "Experiment Detail :: Allen Brain Atlas: Mouse Brain". mouse.brain-map.org. Retrieved 2019-05-15.
  17. . doi:10.7287/peerj.preprints.26870v1/supp-19 //doi.org/10.7287%2Fpeerj.preprints.26870v1%2Fsupp-19. Cite journal requires |journal= (help); Missing or empty |title= (help)
  18. "Figure S10: Putative secondary structures of the four IGRs (A-D correspond to IGRs in Fig. 2 from left to right) from the filiferanHydractinia symbiolongicarpususing the RNA Folding Form of the mfold Web Server (mfold.rna.albany.edu/?q=mfold)". doi:10.7717/peerj.1403/supp-10. Cite journal requires |journal= (help)
  19. "PSORT II Prediction". psort.hgc.jp. Retrieved 2019-05-15.
  20. "PredictProtein - Protein Sequence Analysis, Prediction of Structural and Functional Features". predictprotein.org. Retrieved 2019-05-15.
  21. "Motif Scan". myhits.isb-sib.ch. Retrieved 2019-05-15.
  22. "SIB myhits". SIB.
  23. "The European Bioinformatics Institute < EMBL-EBI". www.ebi.ac.uk. Retrieved 2019-04-22.
  24. "PredictProtein - Protein Sequence Analysis, Prediction of Structural and Functional Features". predictprotein.org. Retrieved 2019-04-22.
  25. "PSORT WWW Server". psort.hgc.jp. Retrieved 2019-04-22.
  26. "ExPaSy (Expert Protein Analysis System)", Encyclopedic Dictionary of Genetics, Genomics and Proteomics, John Wiley & Sons, Inc., 2004-07-15, doi:10.1002/0471684228.egp04300, ISBN 978-0471684220

Further reading
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