TMUB2

Transmembrane and ubiquitin-like domain-containing protein 2 is a protein that in humans is encoded by the TMUB2 gene.[4][5][6]

TMUB2
Identifiers
Aliases	TMUB2, FP2653, transmembrane and ubiquitin like domain containing 2
External IDs	MGI: 1919303 HomoloGene: 18581 GeneCards: TMUB2
Gene location (Mouse) Chr. Chromosome 11 (mouse)[1] Band 11|11 D Start 102,284,931 bp[1] End 102,289,237 bp[1]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding Cellular component • membrane • integral component of membrane Biological process • ubiquitin-dependent ERAD pathway Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	79089	72053
Ensembl	ENSG00000168591	ENSMUSG00000034757
UniProt	Q71RG4	Q3V209
RefSeq (mRNA)	NM_001076674 NM_024107 NM_177441 NM_001330235	NM_028076 NM_001302505 NM_001302506
RefSeq (protein)	NP_001070142 NP_001317164 NP_077012 NP_803190 NP_001340102 NP_001340103 NP_001340104 NP_001340105 NP_001340106 NP_001340107 NP_001340109 NP_001340110 NP_001340111 NP_001340112 NP_001340113 NP_001340114 NP_001340115 NP_001340116 NP_001340117 NP_001340118 NP_001340119 NP_001340120	NP_001289434 NP_001289435 NP_082352
Location (UCSC)	n/a	Chr 11: 102.28 – 102.29 Mb
PubMed search	[2]	[3]
Wikidata
View/Edit Human View/Edit Mouse

Gene

TMUB2 maps on the human chromosome 17, at locus 17q21.31.[6] TMUB2 sits between two neighboring genes, ASB16-AS1 to the left and ATXN7L3 to the right.[7] TMUB2 is 4.99Kb long. The TMUB2 gene can be transcribed into three possible mRNA variants.[8]

Expression

TMUB2 is likely ubiquitously expressed throughout the human body.[9] It has a high expression level that is 2.9 times higher than other human genes.[10][11]

Protein

The TMUB2 protein has a function that is not currently known. It consists of a 321 amino acid long chain in humans. The human protein has a molecular weight of 33.8kdal, an isoelectric point of 4.73899, and three transmembrane regions.[12] These will likely vary in orthologs.

Homology

Paralogs

TMUB1 is the only paralog of TMUB2.[13][14] These proteins share a 38% identity and 51% similarity.[15]

Orthologs

The table below presents a selection of some of the TMUB2 orthologs to display protein diversity among species.[15]

Species	Common name	Accession number	Sequence length (aa)	Sequence identity	Sequence similarity
Pan troglodytes	Chimpanzee	XP_003953053.1	301	100%	100%
Felis Catus	Cat	XP_003997025.1	322	95%	95%
Mus Musculus	Mouse	AAH29841.2	319	85%	88%
Alligator Mississippiensus	Alligator	XP_006271613.1	306	61%	71%
Haliaeetus leucocephalus	Bald Eagle	XP_010559728	301	59%	70%
Danio rerio	Zebrafish	NP_001005573.1	291	47%	60%
Acromyrmex echinatior	Ant	XP_011049429.1	354	23%	42%
Nannochloropsis Gaditana*	Algae	EWM26843.1	476	41%	54%
Coccidioides immitis RS*	Pathogenic Fungus	XP_001242306.1	418	38%	50%

*Limited Query Coverage

Protein Interactions

In humans, Ubiquitin C (UBC) is a protein with a known interaction with TMUB2.[16][17][18][19] Other proposed interactions include BCL2L13 (BCL2-like 13),[20] SGTA (Small glutamine-rich tetratricopeptide repeat-containing protein),[20] and UBQLN1 (Ubiquilin-1).[20][21][22]

References

1. GRCm38: Ensembl release 89: ENSMUSG00000034757 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (June 1996). "A "double adaptor" method for improved shotgun library construction". Anal Biochem. 236 (1): 107–113. doi:10.1006/abio.1996.0138. PMID 8619474.
5. Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (June 1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
6. "Entrez Gene: TMUB2 transmembrane and ubiquitin-like domain containing 2".
7. UCSC Genome Browser: BLAT Search
8. NCBI Gene: TMUB2
9. EST Profile Viewer: Human
10. Aceview: TMUB2
11. NCBI GEO Profiles
12. SDSC Biology Workbench 2.0
13. GeneCards: TMUB2
14. NCBI Gene: TMUB1
15. NCBI BLAST: Basic Local Alignment Search Tool
16. Danielsen Jannie M R; Sylvestersen Kathrine B; Bekker-Jensen Simon; Szklarczyk Damian; Poulsen Jon W; Horn Heiko; Jensen Lars J; Mailand Niels; Nielsen Michael L (2011). "Mass Spectrometric Analysis of Lysine Ubiquitylation Reveals Promiscuity at Site Level". Molecular & Cellular Proteomics. 10 (3): M110.003590. doi:10.1074/mcp.M110.003590. PMC 3047152. PMID 21139048.
17. Wagner S. A.; Beli P.; Weinert B. T.; Nielsen M. L.; Cox J.; Mann M.; Choudhary C. (2011). "A Proteome-Wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles". Molecular & Cellular Proteomics. 10 (10): M111.013284. doi:10.1074/mcp.M111.013284. PMC 3205876. PMID 21890473.
18. Kim Woong; Bennett Eric J.; Huttlin Edward L.; Guo Ailan; Li Jing; Possemato Anthony; Sowa Mathew E.; et al. (2011). "Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome". Molecular Cell. 44 (2): 325–40. doi:10.1016/j.molcel.2011.08.025. PMC 3200427. PMID 21906983.
19. Povlsen Lou K.; Beli Petra; Wagner Sebastian A.; Poulsen Sara L.; Sylvestersen Kathrine B.; Poulsen Jon W.; Nielsen Michael L.; Bekker-Jensen Simon; Mailand Niels; Choudhary Chunaram (2012). "Systems-Wide Analysis of Ubiquitylation Dynamics Reveals a Key Role for PAF15 Ubiquitylation in DNA-Damage Bypass". Nature Cell Biology. 14 (10): 1089–1098. doi:10.1038/ncb2579. PMID 23000965. S2CID 26442522.
20. Rolland T, Taşan M, Charloteaux B, et al. (November 2014). "A proteome-scale map of the human interactome network". Cell. 159 (5): 1212–26. doi:10.1016/j.cell.2014.10.050. PMC 4266588. PMID 25416956.
21. STRING: Functional Protein Association Networks
22. BioGRID Database

Further reading

• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–1795. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–435. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–2270. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–45. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–2144. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Wan D, Gong Y, Qin W, et al. (2004). "Large-scale cDNA transfection screening for genes related to cancer development and progression". Proc. Natl. Acad. Sci. U.S.A. 101 (44): 15724–15729. Bibcode:2004PNAS..10115724W. doi:10.1073/pnas.0404089101. PMC 524842. PMID 15498874.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–D418. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.