TRIM45

tripartite motif containing 45, also known as TRIM45, is a human gene.[5]

TRIM45
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DS4
Identifiers
Aliases	TRIM45, RNF99, tripartite motif containing 45
External IDs	OMIM: 609318 MGI: 1918187 HomoloGene: 11865 GeneCards: TRIM45
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p13.1 Start 117,111,060 bp[1] End 117,122,587 bp[1]
Gene location (Mouse) Chr. Chromosome 3 (mouse)[2] Band 3|3 F2.2 Start 100,922,202 bp[2] End 100,936,920 bp[2]
Gene ontology Molecular function • zinc ion binding • metal ion binding Cellular component • intracellular • cell nucleus • nucleoplasm • intercellular bridge • cytoplasm • cytosol Biological process • bone development Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	80263	229644
Ensembl	ENSG00000134253	ENSMUSG00000033233
UniProt	Q9H8W5	Q6PFY8
RefSeq (mRNA)	NM_001145635 NM_025188	NM_001165952 NM_001165953 NM_194343
RefSeq (protein)	NP_001139107 NP_079464	NP_001159424 NP_001159425 NP_919324
Location (UCSC)	Chr 1: 117.11 – 117.12 Mb	Chr 3: 100.92 – 100.94 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a member of the tripartite motif family. The encoded protein may function as a [transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[5]

Model organisms

Trim45 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Abnormal[6]
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
MicroCT & Quantitative Faxitron	Abnormal
Salmonella infection	Normal[7]
Citrobacter infection	Normal[8]
All tests and analysis from[9][10]

Model organisms have been used in the study of TRIM45 function. A conditional knockout mouse line, called Trim45^{tm1a(KOMP)Wtsi}[11][12] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[13][14][15]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty six tests were carried out on mutant mice and three significant abnormalities were observed.[9] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; males had increased circulating magnesium levels while animals of both sex displayed increased bone strength.[9]

References

1. GRCh38: Ensembl release 89: ENSG00000134253 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000033233 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: tripartite motif containing 45". Retrieved 2011-08-30.
6. "Clinical chemistry data for Trim45". Wellcome Trust Sanger Institute.
7. "Salmonella infection data for Trim45". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Trim45". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
14. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
15. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Sardiello M, Cairo S, Fontanella B, Ballabio A, Meroni G (2008). "Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties". BMC Evolutionary Biology. 8: 225. doi:10.1186/1471-2148-8-225. PMC 2533329. PMID 18673550.
• Wang Y, Li Y, Qi X, Yuan W, Ai J, Zhu C, Cao L, Yang H, Liu F, Wu X, Liu M (Oct 2004). "TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1". Biochemical and Biophysical Research Communications. 323 (1): 9–16. doi:10.1016/j.bbrc.2004.08.048. PMID 15351693.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.