TTLL3

Tubulin tyrosine ligase-like family, member 3 is a protein that in humans is encoded by the TTLL3 gene.[5]

TTLL3
Identifiers
Aliases	TTLL3, HOTTL, tubulin tyrosine ligase like 3
External IDs	MGI: 2141418 HomoloGene: 134361 GeneCards: TTLL3
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p25.3 Start 9,808,086 bp[1] End 9,855,138 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6|6 E3 Start 113,389,260 bp[2] End 113,414,587 bp[2]
Gene ontology Molecular function • ATP binding • protein-glycine ligase activity, initiating • nucleotide binding • ligase activity • protein-glycine ligase activity Cellular component • microtubules • GO:0035085 axoneme • microtubule cytoskeleton • cytoplasm • cell projection • cilium • cytoskeleton • cytosol Biological process • axoneme assembly • cellular protein modification process • cilium morphogenesis • protein polyglycylation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	26140	101100
Ensembl	ENSG00000214021	ENSMUSG00000030276
UniProt	Q9Y4R7	A4Q9E5
RefSeq (mRNA)	NM_001025930 NM_015644 NM_001366051	NM_001142732 NM_133923
RefSeq (protein)	NP_001021100 NP_001352980	NP_001136204 NP_598684
Location (UCSC)	Chr 3: 9.81 – 9.86 Mb	Chr 6: 113.39 – 113.41 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Ttll3 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Brain histopathology	Normal
Salmonella infection	Normal[6]
Citrobacter infection	Normal[7]
All tests and analysis from[8][9]

Model organisms have been used in the study of TTLL3 function. A conditional knockout mouse line, called Ttll3^{tm1a(EUCOMM)Wtsi}[10][11] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[12][13][14]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][15] Twenty three tests were carried out on mutant mice but no significant abnormalities were observed.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000214021 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030276 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: Tubulin tyrosine ligase-like family, member 3". Retrieved 2011-09-21.
6. "Salmonella infection data for Ttll3". Wellcome Trust Sanger Institute.
7. "Citrobacter infection data for Ttll3". Wellcome Trust Sanger Institute.
8. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
9. Mouse Resources Portal, Wellcome Trust Sanger Institute.
10. "International Knockout Mouse Consortium".
11. "Mouse Genome Informatics".
12. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
13. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
14. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
15. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Trichet, V.; Ruault, M.; Roizès, G.; De Sario, A. (2000). "Characterization of the human tubulin tyrosine ligase-like 1 gene (TTLL1) mapping to 22q13.1". Gene. 257 (1): 109–117. doi:10.1016/S0378-1119(00)00383-8. PMID 11054573.
• Rogowski, K.; Juge, F. O.; Van Dijk, J.; Wloga, D.; Strub, J. M.; Levilliers, N.; Thomas, D.; Bré, M. H. L. N.; Van Dorsselaer, A.; Gaertig, J.; Janke, C. (2009). "Evolutionary Divergence of Enzymatic Mechanisms for Posttranslational Polyglycylation" (PDF). Cell. 137 (6): 1076–1087. doi:10.1016/j.cell.2009.05.020. PMID 19524510. S2CID 953190.