UDP-sulfoquinovose synthase
UDP-sulfoquinovose synthase (EC 3.13.1.1) is an enzyme that catalyzes the chemical reaction
- UDP-glucose + sulfite UDP-6-sulfoquinovose + H2O
| UDPsulfoquinovose synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.13.1.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are UDP-glucose and sulfite, whereas its two products are UDP-6-sulfoquinovose and H2O.[1]
In a subsequent reaction catalyzed by sulfoquinovosyl diacylglycerol synthase, the sulfoquinovose portion of UDP-sulfoquinovose is combined with diacyglycerol to produce the sulfolipid sulfoquinovosyl diacylglycerol (SQDG).[2]
This enzyme belongs to the family of hydrolases, specifically those acting on carbon-sulfur bonds. The systematic name of this enzyme class is UDP-6-sulfo-6-deoxyglucose sulfohydrolase. Other names in common use include sulfite:UDP-glucose sulfotransferase, and UDP-sulfoquinovose synthase. This enzyme participates in nucleotide sugars metabolism and glycerolipid metabolism.
The 3-dimensional structure of the enzyme is known from Protein Data Bank entries 1qrr (Mulichak et al., 1999), 1i24, 1i2b and 1i2c.
See also
References
- Mulichak, AM; Theisen, MJ; Essigmann, B; Benning, C; Garavito, RM (9 November 1999). "Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose". Proceedings of the National Academy of Sciences of the United States of America. 96 (23): 13097–102. Bibcode:1999PNAS...9613097M. doi:10.1073/pnas.96.23.13097. PMC 23906. PMID 10557279.
- Goddard-Borger, ED; Williams, SJ (20 February 2017). "Sulfoquinovose in the biosphere: occurrence, metabolism and functions". The Biochemical Journal. 474 (5): 827–849. doi:10.1042/BCJ20160508. PMID 28219973.
- Essigmann B, Guler S, Narang RA, Linke D, Benning C (1998). "Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana". Proc. Natl. Acad. Sci. U.S.A. 95 (4): 1950–5. Bibcode:1998PNAS...95.1950E. doi:10.1073/pnas.95.4.1950. PMC 19220. PMID 9465123.
- Essigmann B, Hespenheide BM, Kuhn LA, Benning C (1999). "Prediction of the active-site structure and NAD(+) binding in SQD1, a protein essential for sulfolipid biosynthesis in Arabidopsis". Arch. Biochem. Biophys. 369 (1): 30–41. doi:10.1006/abbi.1999.1344. PMID 10462438.
- Sanda S, Leustek T, Theisen MJ, Garavito RM, Benning C (2001). "Recombinant Arabidopsis SQD1 converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose in vitro". J. Biol. Chem. 276 (6): 3941–6. doi:10.1074/jbc.M008200200. PMID 11073956.