ZNF367

Zinc finger protein 367 is a protein that in humans is encoded by the ZNF367 gene.[1] The human gene is also known as ZFF29 and CDC14B; the orthologue in mice is Zfp367.[1] ZNF367 contains a unique Cys2His2 zinc finger motif and is a member of the zinc finger protein family.[2]

Model organisms

Zfp367 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of ZNF367 function. A conditional knockout mouse line, called Zfp367^{tm1a(KOMP)Wtsi}[7][8] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][12] Twenty six tests were carried out on mutant mice, but no significant abnormalities were observed.[5]

References

1. "Zinc finger protein 367". Retrieved 2011-12-07.
2. Murate, T.; Asano, H.; Naoe, T.; Saito, H.; Stamatoyannopoulos, G. (2004). "Molecular cloning and characterization of ZFF29: A protein containing a unique Cys2His2 zinc-finger motif". Biochemical Journal. 384 (3): 647–653. doi:10.1042/BJ20040394. PMC 1134151. PMID 15344908.
3. "Salmonella infection data for Zfp367". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Zfp367". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Gilligan, P.; Brenner, S.; Venkatesh, B. (2002). "Fugu and human sequence comparison identifies novel human genes and conserved non-coding sequences". Gene. 294 (1–2): 35–44. doi:10.1016/S0378-1119(02)00793-X. PMID 12234665.