D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase (EC 2.4.1.264, GumK) is an enzyme with systematic name UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol UDP + D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Identifiers
EC number2.4.1.264
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

References
  1. Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Pühler A, Ielpi L (April 1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". Journal of Bacteriology. 180 (7): 1607–17. PMC 107069. PMID 9537354.
  2. Ielpi L, Couso RO, Dankert MA (May 1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". Journal of Bacteriology. 175 (9): 2490–500. PMC 204549. PMID 7683019.
  3. Kim SY, Kim JG, Lee BM, Cho JY (February 2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnology Letters. 31 (2): 265–70. doi:10.1007/s10529-008-9858-3. PMID 18854951.
  4. Barreras M, Bianchet MA, Ielpi L (September 2006). "Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. 62 (Pt 9): 880–3. doi:10.1107/S1744309106028764. PMC 2242869. PMID 16946469.
  5. Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L (September 2008). "Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase". The Journal of Biological Chemistry. 283 (36): 25027–35. doi:10.1074/jbc.M801227200. PMC 3259810. PMID 18596046.
  6. Vojnov AA, Bassi DE, Daniels MJ, Dankert MA (February 2002). "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris". Carbohydrate Research. 337 (4): 315–26. doi:10.1016/S0008-6215(01)00322-6. PMID 11841812.
  7. Barreras M, Abdian PL, Ielpi L (March 2004). "Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Glycobiology. 14 (3): 233–41. doi:10.1093/glycob/cwh056. PMID 14736729.
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