Deoxycytidine triphosphate
Deoxycytidine triphosphate (dCTP) is a nucleoside triphosphate that contains the pyrimidine base cytosine. The triphosphate group contains high-energy phosphoanhydride bonds, which liberate energy when hydrolized.
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ECHA InfoCard | 100.016.504 |
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C9H16N3O13P3 | |
Molar mass | 467.156 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
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DNA polymerase enzymes use this energy to incorporate deoxycytidine into a newly synthesized strand of DNA. A chemical equation can be written that represents the process:
- (DNA)n + dCTP ↔ (DNA)n-C + PPi
That is, dCTP has the PPi (pyrophosphate) cleaved off and the dCMP is incorporated into the DNA strand at the 3' end. Subsequent hydrolysis of the PPi drives the equilibrium of the reaction toward the right side, i.e. incorporation of the nucleotide in the growing DNA chain.
Like other nucleoside triphosphates, manufacturers recommend that dCTP be stored in aqueous solution at −20 °C.[1]
See also
References
- "Definitive Guide to dNTPs" (PDF). Retrieved July 29, 2017.