EIF2C2

Protein argonaute-2 is a protein that in humans is encoded by the EIF2C2 gene.[4][5][6]

AGO2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3LUC, 3LUD, 3LUG, 3LUH, 3LUJ, 3LUK, 3QX8, 3QX9, 4F3T, 4OLA, 4OLB, 4W5N, 4W5O, 4W5Q, 4W5R, 4W5T, 4Z4C, 4Z4D, 4Z4E, 4Z4F, 4Z4G, 4Z4H, 4Z4I, 5JS1, 5JS2
Identifiers
Aliases	AGO2, EIF2C2, Q10, argonaute 2, RISC catalytic component, PPD, CASC7, LINC00980, argonaute RISC catalytic component 2
External IDs	OMIM: 606229 MGI: 2446632 HomoloGene: 81825 GeneCards: AGO2
Gene location (Human) Chr. Chromosome 8 (human)[1] Band 8q24.3 Start 140,520,156 bp[1] End 140,635,633 bp[1]
Gene ontology Molecular function • GO:0001948 protein binding • protein C-terminus binding • metal ion binding • nuclease activity • endoribonuclease activity, cleaving miRNA-paired mRNA • hydrolase activity • translation initiation factor activity • endonuclease activity • endoribonuclease activity, cleaving siRNA-paired mRNA • single-stranded RNA binding • double-stranded RNA binding • RNA polymerase II core binding • nucleic acid binding • mRNA binding • miRNA binding • endoribonuclease activity • RNA 7-methylguanosine cap binding • siRNA binding • mRNA cap binding • RNA binding • endoribonuclease activity, producing 5'-phosphomonoesters Cellular component • cytoplasm • polysome • membrane • P-body • dendrite • RISC-loading complex • RISC complex • cytosol • cell nucleus • nucleoplasm • mRNA cap binding complex • cell junction • extracellular exosome • ribonucleoprotein Biological process • mRNA cleavage involved in gene silencing by miRNA • RNA secondary structure unwinding • transcription, DNA-templated • regulation of translation • posttranscriptional gene silencing by RNA • protein biosynthesis • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • positive regulation of gene expression • production of miRNAs involved in gene silencing by miRNA • miRNA loading onto RISC involved in gene silencing by miRNA • mRNA cleavage involved in gene silencing by siRNA • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening • pre-miRNA processing • regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • post-embryonic development • siRNA loading onto RISC involved in RNA interference • RNA phosphodiester bond hydrolysis, endonucleolytic • translational initiation • Wnt signaling pathway, calcium modulating pathway • miRNA mediated inhibition of translation • negative regulation of translational initiation • positive regulation of miRNA mediated inhibition of translation • miRNA metabolic process • production of siRNA involved in RNA interference • gene silencing by RNA • regulation of gene silencing by miRNA • negative regulation of gene expression • positive regulation of angiogenesis • positive regulation of trophoblast cell migration • negative regulation of amyloid precursor protein biosynthetic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	27161	239528
Ensembl	ENSG00000123908	ENSMUSG00000036698
UniProt	Q9UKV8	Q8CJG0
RefSeq (mRNA)	NM_001164623 NM_012154	NM_153178
RefSeq (protein)	NP_001158095 NP_036286	NP_694818
Location (UCSC)	Chr 8: 140.52 – 140.64 Mb	n/a
PubMed search	[2]	[3]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with Dicer1 and play a role in short-interfering-RNA-mediated gene silencing.[6]

Interactions

EIF2C2 has been shown to interact with

• DDX20,[7][8]
• DICER1,[8][9]
• FMRP, FXR1P, FXR2P,[10] and
• TNRC6B.[11]

References

1. GRCh38: Ensembl release 89: ENSG00000123908 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Koesters R, Adams V, Betts D, Moos R, Schmid M, Siermann A, Hassam S, Weitz S, Lichter P, Heitz PU, von Knebel Doeberitz M, Briner J (Dec 1999). "Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression". Genomics. 61 (2): 210–8. doi:10.1006/geno.1999.5951. PMID 10534406.
5. Sasaki T, Shiohama A, Minoshima S, Shimizu N (Aug 2003). "Identification of eight members of the Argonaute family in the human genome small star, filled". Genomics. 82 (3): 323–30. doi:10.1016/S0888-7543(03)00129-0. PMID 12906857.
6. "Entrez Gene: EIF2C2 eukaryotic translation initiation factor 2C, 2".
7. Nelson PT, Hatzigeorgiou AG, Mourelatos Z (March 2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384.
8. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G (March 2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277.
9. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K (January 2003). "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors". Curr. Biol. 13 (1): 41–6. doi:10.1016/S0960-9822(02)01394-5. PMID 12526743. S2CID 14650075.
10. Jin P, Zarnescu DC, Ceman S, Nakamoto M, Mowrey J, Jongens TA, Nelson DL, Moses K, Warren ST (February 2004). "Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway". Nat. Neurosci. 7 (2): 113–7. doi:10.1038/nn1174. PMID 14703574. S2CID 1146182.
11. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, Heinrich C, Hentze MW, Ladurner AG (October 2007). "A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain". Nat. Struct. Mol. Biol. 14 (10): 897–903. doi:10.1038/nsmb1302. PMID 17891150. S2CID 8136380.

Further reading

• Carmell MA, Xuan Z, Zhang MQ, Hannon GJ (2002). "The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis". Genes Dev. 16 (21): 2733–42. doi:10.1101/gad.1026102. PMID 12414724.
• Mourelatos Z, Dostie J, Paushkin S, et al. (2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277.
• Martinez J, Patkaniowska A, Urlaub H, et al. (2003). "Single-stranded antisense siRNAs guide target RNA cleavage in RNAi". Cell. 110 (5): 563–74. doi:10.1016/S0092-8674(02)00908-X. hdl:11858/00-001M-0000-0012-F2FD-2. PMID 12230974. S2CID 10616773.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Doi N, Zenno S, Ueda R, et al. (2003). "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors". Curr. Biol. 13 (1): 41–6. doi:10.1016/S0960-9822(02)01394-5. PMID 12526743. S2CID 14650075.
• Tahbaz N, Kolb FA, Zhang H, et al. (2004). "Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer". EMBO Rep. 5 (2): 189–94. doi:10.1038/sj.embor.7400070. PMC 1298981. PMID 14749716.
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z (2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384.
• Liu J, Carmell MA, Rivas FV, et al. (2004). "Argonaute2 is the catalytic engine of mammalian RNAi". Science. 305 (5689): 1437–41. doi:10.1126/science.1102513. PMID 15284456. S2CID 2778088.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rivas FV, Tolia NH, Song JJ, et al. (2005). "Purified Argonaute2 and an siRNA form recombinant human RISC". Nat. Struct. Mol. Biol. 12 (4): 340–9. doi:10.1038/nsmb918. PMID 15800637. S2CID 2021813.
• Sen GL, Blau HM (2005). "Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies". Nat. Cell Biol. 7 (6): 633–6. doi:10.1038/ncb1265. PMID 15908945. S2CID 6085169.
• Liu J, Valencia-Sanchez MA, Hannon GJ, Parker R (2005). "MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies". Nat. Cell Biol. 7 (7): 719–23. doi:10.1038/ncb1274. PMC 1855297. PMID 15937477.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Kraynack BA, Baker BF (2006). "Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity". RNA. 12 (1): 163–76. doi:10.1261/rna.2150806. PMC 1370895. PMID 16301602.
• Maniataki E, Mourelatos Z (2006). "A human, ATP-independent, RISC assembly machine fueled by pre-miRNA". Genes Dev. 19 (24): 2979–90. doi:10.1101/gad.1384005. PMC 1315402. PMID 16357216.
• Shi H, Tschudi C, Ullu E (2006). "Functional replacement of Trypanosoma brucei Argonaute by the human slicer Argonaute2". RNA. 12 (6): 943–7. doi:10.1261/rna.20806. PMC 1464859. PMID 16611939.
• Wichroski MJ, Robb GB, Rana TM (2006). "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies". PLOS Pathog. 2 (5): e41. doi:10.1371/journal.ppat.0020041. PMC 1458959. PMID 16699599.