GelGreen

GelGreen is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelGreen consists of two acridine orange subunits that are bridged by a linear oxygenated spacer.[1][2]

GelGreen
Names
IUPAC name
10,10'-(6,22-dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis(3,6-bis(dimethylamino)acridin-10-ium) iodide
Other names
Dye No. 20,[1] AOAO-13[1]
Identifiers
3D model (JSmol)
ChemSpider
Properties
C56H80I2N8O5
Molar mass 1198.43 g/mol
Hazards
Safety data sheet 10,000X in water, Biotium Inc.
R-phrases (outdated) R25 R36/37/38
S-phrases (outdated) S22 S24/25 S26 S36/37/39 S45 S53
NFPA 704 (fire diamond)
Flammability code 0: Will not burn. E.g. waterHealth code 0: Exposure under fire conditions would offer no hazard beyond that of ordinary combustible material. E.g. sodium chlorideReactivity code 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
0
0
0
Flash point > 100 °C (212 °F; 373 K)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelGreen is sold as a solution in either DMSO or water.[3]

See also

References
  1. US application 2010323453, Mao, Fei & Leung, Wai-Yee, "Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology"
  2. GelRed & GelGreen (PDF), Biotium Inc., August 21, 2012, retrieved December 4, 2012
  3. GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., retrieved December 4, 2012
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