GelRed
GelRed is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelRed structurally consists of two ethidium subunits that are bridged by a linear oxygenated spacer.[1][2]
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| IUPAC name
5,5'-(6,22-dioxo-11,14,17-trioxa-7,21-diazaheptacosane-1,27-diyl)bis(3,8-diamino-6-phenylphenanthridin-5-ium) iodide | |
| Other names | |
| Identifiers | |
3D model (JSmol) |
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PubChem CID |
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| Properties | |
| C60H72I2N8O5 | |
| Molar mass | 1239.07 g/mol |
| Hazards | |
| Safety data sheet | 10,000X in water, Biotium Inc. |
| R-phrases (outdated) | R25 R36/37/38 |
| S-phrases (outdated) | S22 S24/25 S26 S36/37/39 S45 S53 |
| NFPA 704 (fire diamond) | |
| Flash point | > 100 °C (212 °F; 373 K) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Its fluorophore, and therefore its optical properties, are essentially identical to those of ethidium bromide. When exposed to ultraviolet light, it will fluoresce with an orange color that strongly intensifies after binding to DNA.[3] The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide.[3] GelRed is sold as a solution in anhydrous DMSO or ultrapurified water.[3]
See also
References
- US application 2010323453, Mao, Fei & Leung, Wai-Yee, "Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology"
- GelRed & GelGreen (PDF), Biotium Inc., August 21, 2012, retrieved December 4, 2012
- GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., retrieved December 4, 2012
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