Giemsa stain

Giemsa stain (/ˈɡiːmzə/), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.[1]

Giemsa stained Trypanosoma parasites (Chagas disease pathogen)
Whirling disease section stained with Giemsa stain

Uses

It is specific for the phosphate groups of DNA and attaches itself to regions of DNA where there are high amounts of adenine-thymine bonding. Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create a karyogram (chromosome map). It can identify chromosomal aberrations such as translocations and rearrangements.

It stains the trophozoite Trichomonas vaginalis, which presents with greenish discharge and motile cells on wet prep.

Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain. It can be used to study the adherence of pathogenic bacteria to human cells. It differentially stains human and bacterial cells purple and pink respectively. It can be used for histopathological diagnosis of the Plasmodium species that cause malaria[2] and some other spirochete and protozoan blood parasites. It is also used in Wolbachia cell stain in Drosophila melanogaster.

Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens. Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta. It is also used to visualize the classic "safety pin" shape in Yersinia pestis.

"Owl's-eye" viral inclusions, associated with Cytomegalovirus infection.

Giemsa stain is also used to visualize chromosomes. This is particularly relevant for detection of Cytomegalovirus infection, where the classical finding would be an "owl-eye" viral inclusion.[3]

Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells.[4]

Generation

Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder.

A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.[5]

See also

References
  1. Giemsa G (1904 Eine Vereinfachung und Vervollkommnung meiner Methylenblau-Eosin-Färbemethode zur Erzielung der Romanowsky-Nocht’schen Chromatinfärbung. Centralblatt für Bakteriologie I Abteilung 32, 307–313.
  2. Shapiro HM, Mandy F (September 2007). "Cytometry in malaria: moving beyond Giemsa". Cytometry Part A. 71 (9): 643–5. doi:10.1002/cyto.a.20453. PMID 17712779. S2CID 12831277.
  3. Woods, G. L.; Walker, D. H. (July 1996). "Detection of infection or infectious agents by use of cytologic and histologic stains". Clinical Microbiology Reviews. 9 (3): 382–404. doi:10.1128/cmr.9.3.382. ISSN 0893-8512. PMC 172900. PMID 8809467.
  4. Damsgaard TE, Olesen AB, Sørensen FB, Thestrup-Pedersen K, Schiøtz PO (April 1997). "Mast cells and atopic dermatitis. Stereological quantification of mast cells in atopic dermatitis and normal human skin". Arch. Dermatol. Res. 289 (5): 256–60. doi:10.1007/s004030050189. PMID 9164634. S2CID 229476. Archived from the original on 2013-02-12.
  5. "4.2.2.2. Giemsa stain". impact-malaria.com. Archived from the original on 2013-10-29. Retrieved 28 Oct 2013.
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