Lillie's trichrome

Lillie's trichrome is a combination of dyes used in histology.

It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940.[1] It is applied by submerging the fixated sample into the following three Solutions:[2]

1) Weigert's Iron Hematoxylin working solution

2) Biebrich Scarlet solution

3) Fast Green FCF solution

The resulting stains are black cell nuclei, brown cytoplasm, red muscle and myelinated fibers, blue collagen, and scarlet erythrocytes.[3]


Standard Applications: Trichrome stains are normally used to differentiate between collagen and muscle tissues. Some studies that benefit from its application include end stage liver disease (cirrhosis), myocardial infarction, muscular dystrophy, and tumor analysis. [4]

References
  1. Lillie, R. D. (1940). "Further Experiments with the Masson Trichrome Modification of Mallory's Connective Tissue Stain". Stain Technology. 15 (1). doi:10.3109/10520294009110327.
  2. Tchertkoff, Victor (1962-10-01). "Staining Procedures Used by the Biological Stain Commission, ed. 2. Revised by H. J. Conn, et al. Baltimore, Md., The Williams & Wilkins Co., 1960. $5.00". Clinical Chemistry. 8 (5): 548–548. doi:10.1093/clinchem/8.5.548a. ISSN 0009-9147.
  3. LILLIE, WALTER I. (1940-11-01). "PRECHIASMAL SYNDROME PRODUCED BY CHRONIC LOCAL ARACHNOIDITIS". Archives of Ophthalmology. 24 (5): 940. doi:10.1001/archopht.1940.00870050090006. ISSN 0093-0326.
  4. Cowie, Robert. "Masson's Trichrome Stain". Science Direct.


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