List of restriction enzyme cutting sites: Bst–Bv

This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains approximately 200 enzymes.

Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 

Whole list navigation

Restriction enzymes

Bst
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
BstI Bacillus stearothermophilus 1503-4R 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132II
Bst1I Bacillus stearothermophilus 1 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI
Bst2I Bacillus stearothermophilus 2 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaI
Bst6I Bacillus stearothermophilus 6 5' CTCTTC
3' GAGAAG 		5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bst11I Bacillus stearothermophilus 11 5' ACTGG
3' TGACC 		5' ---ACTGGN   --- 3'
3' ---TGAC   CN--- 5'
Bst12I Bacillus stearothermophilus 12 5' GCAGC
3' CGTCG 		5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5' 		AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1I
Bst19I Bacillus stearothermophilus 19 5' GCATC
3' CGTAG 		5' ---GCATCNNNN   NN--- 3'
3' ---CGTAGNNNNNN   --- 5'
Bst19II Bacillus stearothermophilus 19 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI
Bst28I Bacillus stearothermophilus 28 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoI
Bst38I Bacillus stearothermophilus 38 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaI
Bst40I Bacillus stearothermophilus 40 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Bst71I Bacillus stearothermophilus 71 5' GCAGC
3' CGTCG 		5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5' 		AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1I
Bst98I Bacillus stearothermophilus C98 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
Bst100I Bacillus stearothermophilus 100 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI
Bst1107I Bacillus stearothermophilus RFL1107 5' GTATAC
3' CATATG 		5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
BstACI Bacillus stearothermophilus AC 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92I
BstAPI Bacillus stearothermophilus AP 5' GCAN4NTGC
3' CGTN4NACG 		5' ---GCANNNN   NTGC--- 3'
3' ---CGTN   NNNNACG--- 5' 		ApaBI
BstAUI Bacillus stearothermophilus AU 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I, BsrGI,
Ssp4800I, SspBI
BstBI Bacillus stearothermophilus B225 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFI
Bst2BI Bacillus stearothermophilus 2B 5' CACGAG
3' GTGCTC 		5' ---C   ACGAG--- 3'
3' ---GTGCT   C--- 5'
BstBAI Bacillus stearothermophilus BA 5' YACGTR
3' RTGCAY 		5' ---YAC   GTR--- 3'
3' ---RTG   CAY--- 5'
BstBSI Bacillus stearothermophilus BS 5' GTATAC
3' CATATG 		5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
BstB7SI Bacillus stearothermophilus B7S 5' RCCGGY
3' YGGCCR 		5' ---R   CCGGY--- 3'
3' ---YGGCC   R--- 5'
BstBS32I Bacillus stearothermophilus BS32 5' GAAGAC
3' CTTCTG 		5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
BstBZ153I Bacillus stearothermophilus BZ153 5' GCGCGC
3' CGCGCG 		5' ---G   CGCGC--- 3'
3' ---CGCGC   G--- 5'
Bst4CI Bacillus stearothermophilus 4C 5' ACNGT
3' TGNCA 		5' ---ACN   GT--- 3'
3' ---TG   NCA--- 5'
BstC8I Bacillus stearothermophilus C8 5' GCNNGC
3' CGNNCG 		5' ---GCN   NGC--- 3'
3' ---CGN   NCG--- 5'
BstD102I Bacillus stearothermophilus D102 5' CCGCTC
3' GGCGAG 		5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5' 		AccBSI, BsrBI, Bst31NI, MbiI
BstDEI Bacillus stearothermophilus DE 5' CTNAG
3' GANTC 		5' ---C   TNAG--- 3'
3' ---GANT   C--- 5'
BstDSI Bacillus stearothermophilus DS 5' CCRYGG
3' GGYRCC 		5' ---C   CRYGG--- 3'
3' ---GGYRC   C--- 5'
BstEII Bacillus stearothermophilus ET 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI
BstENI Bacillus stearothermophilus EN 5' CCTN4NAGG
3' GGAN4NTCC 		5' ---CCTNN   NNNAGG--- 3'
3' ---GGANNN   NNTCC--- 5'
BstENII Bacillus stearothermophilus EN 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI
BstEZ359I Bacillus stearothermophilus EZ359 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
BstFI Bacillus stearothermophilus FH58 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
BstF5I Bacillus stearothermophilus F5 5' GGATG
3' CCTAC 		5' ---GGATGNN   --- 3'
3' ---CCTAC   NN--- 5'
BstFNI Bacillus stearothermophilus 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelI
BstFZ438I Bacillus stearothermophilus FZ438 5' CCCGC
3' GGGCG 		5' ---CCCGCNNNN   NN--- 3'
3' ---GGGCGNNNNNN   --- 5'
BstGZ53I Bacillus stearothermophilus GZ53 5' CGTCTC
3' GCAGAG 		5' ---CGTCTCN   NNNN--- 3'
3' ---GCAGAGNNNNN   --- 5'
BstH2I Bacillus stearothermophilus H2 5' RGCGCY
3' YCGCGR 		5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5'
AccB2I,  BfoI,  Bme142I,
Bsp143II, HaeII, LpnI
BstH9I Bacillus stearothermophilus H9 5' GGATC
3' CCTAG 		5' ---GGATCNNNN   N--- 3'
3' ---CCTAGNNNNN   --- 5'
AclWI, AlwI, BinI,  BpuFI,  BspPI,
 Bst31TI, BthII, Bth617I,  EacI
BstHHI Bacillus stearothermophilus HH 5' GCGC
3' CGCG 		5' ---GCG   C--- 3'
3' ---C   GCG--- 5' 		AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
BstHPI Bacillus stearothermophilus HP 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
BstHZ55I Bacillus stearothermophilus HZ55 5' CCAN6TGG
3' GGTN6ACC 		5' ---CCANNNNN   NTGG--- 3'
3' ---GGTN   NNNNNACC--- 5'
BstIZ316I Bacillus stearothermophilus IZ316 5' CACNNNGTG
3' GTGNNNCAC 		5' ---CACNNN   GTG--- 3'
3' ---GTG   NNNCAC--- 5' 		AdeI, DraIII
BstJZ301I Bacillus stearothermophilus JZ301 5' CTNAG
3' GANTC 		5' ---C   TNAG--- 3'
3' ---GANT   C--- 5'
BstKTI Bacillus stearothermophilus KT 5' GATC
3' CTAG 		5' ---GAT   C--- 3'
3' ---C   TAG--- 5' 		AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAI
BstM6I Bacillus stearothermophilus M6 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaI
BstMAI Bacillus stearothermophilus MA 5' GTCTC
3' CAGAG 		5' ---GTCTCN   NNNNN--- 3'
3' ---CAGAGNNNNN   N--- 5'
Alw26I,  BcoDI, BscQII,  BsmAI,
BsoMAI
BstMBI Bacillus stearothermophilus MB 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I
BstMCI Bacillus stearothermophilus MC 5' CGRYCG
3' GCYRGC 		5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
BstMWI Bacillus stearothermophilus MW 5' GCN7GC
3' CGN7CG 		5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
BstMZ611I Bacillus stearothermophilus MZ611 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
BstNI Bacillus stearothermophilus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117I
Bst31NI Bacillus stearothermophilus 31N 5' CCGCTC
3' GGCGAG 		5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5' 		AccBSI, BsrBI, BstD102I, MbiI
BstNSI Bacillus stearothermophilus 1161NS 5' RCATGY
3' YGTACR 		5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
BstNZ169I Bacillus stearothermophilus NZ169 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoI
BstOI Bacillus stearothermophilus O22 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117I
BstOZ616I Bacillus stearothermophilus OZ616 5' GGGAC
3' CCCTG 		5' ---GGGACN8NN   NNNN--- 3'
3' ---CCCTGN8NNNNNN   --- 5'
BstPI Bacillus stearothermophilus 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI
BstPAI Bacillus stearothermophilus PA 5' GACN4GTC
3' CTGN4CAG 		5' ---GACNN   NNGTC--- 3'
3' ---CTGNN   NNCAG--- 5'
BstPZ418I 5' GGATG
3' CCTAC 		5' ---GGATGN8N   NNNN--- 3'
3' ---CCTACN8NNNNN   --- 5'
BstPZ740I Bacillus stearothermophilus PZ740 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
BstRZ246I Bacillus stearothermophilus RZ246 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
BstSI Bacillus stearothermophilus S183 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAI
BstSCI Bacillus stearothermophilus SC 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
BstSFI Bacillus stearothermophilus SF 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
BstSNI Bacillus stearothermophilus SN 5' TACGTA
3' ATGCAT 		5' ---TAC   GTA--- 3'
3' ---ATG   CAT--- 5'
BstSWI Bacillus stearothermophilus SW 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
BstT7I Bacillus stearothermophilus T7 5' TGATCA
3' ACTAGT 		5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BclI, BsiQI,
BspXII, FbaI, Ksp22I,  ParI 
BstT9I Bacillus stearothermophilus T9 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEI
BstT10I Bacillus stearothermophilus T10 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEI
Bst31TI Bacillus stearothermophilus 31T 5' GGATC
3' CCTAG 		5' ---GGATCNNNN   N--- 3'
3' ---CCTAGNNNNN   --- 5'
BstTS5I Bacillus stearothermophilus TS5 5' GAAGAC
3' CTTCTG 		5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
BstUI Bacillus stearothermophilus U458 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelI
Bst2UI Bacillus stearothermophilus 2U 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanI
BstVI Bacillus stearothermophilus V 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoI
BstV1I Bacillus stearothermophilus V1 5' GCAGC
3' CGTCG 		5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5' 		AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I
BstV2I Bacillus stearothermophilus V2 5' GAAGAC
3' CTTCTG 		5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
BstXI Bacillus stearothermophilus X1 5' CCAN6TGG
3' GGTN6ACC 		5' ---CCANNNNN   NTGG--- 3'
3' ---GGTN   NNNNNACC--- 5'
BstX2I Bacillus stearothermophilus X2 5' RGATCY
3' YCTAGR 		5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
BstYI 1SDO Bacillus stearothermophilus Y406 5' RGATCY
3' YCTAGR 		5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
BstZI Bacillus stearothermophilus Z130 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIII
BstZ17I Bacillus stearothermophilus 38M 5' GTATAC
3' CATATG 		5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
This list is incomplete; you can help by adding missing items with reliable sources.

Bsu - Bv
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Bsu6I Bacillus subtilis 6v1 5' CTCTTC
3' GAGAAG 		5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Bsu15I Bacillus subtilis 15 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, BsuTUI, ClaI
Bsu23I Bacillus subtilis 23 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		AccIII, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
Bsu36I Bacillus subtilis 36 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Bsu54I Bacillus subtilis 54 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI
Bsu1532I Bacillus subtilis 1532 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		BceBI, Bpu95I, Bsp123I, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI
Bsu1854I Bacillus subtilis 1854 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
BsuBI Bacillus subtilis 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AjoI, BloHII, CflI, CstI, Ecl37kI, HalII, Psp23I, PstI, SalPI, SflI, YenI
BsuFI Bacillus subtilis 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
BsuMI Bacillus subtilis 168 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		XhoI, BssHI, TliI, PaeR7I, Sfr274I, SlaI, StrI
BsuRI Bacillus subtilis R 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5' 		HaeIII
BsuTUI Bacillus subtilis TU 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, ClaI
BteI Brochothrix thermosphacter 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
BtgI Bacillus thermoglucosidasius 5' CCRYGG
3' GGYRCC 		5' ---C   CRYGG--- 3'
3' ---GGYRC   C--- 5'
BtgZI Bacillus thermoglucosidasius 5' GCGATG
3' CGCTAC 		5' ---GCGATGN8NN   NNNN--- 3'
3' ---CGCTACN8NNNNNN   --- 5'
BthAI Bacillus thuringiensis AII 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, HgiJI, SmuEI
BthCI Bacillus thuringiensis 2294 5' GCNGC
3' CGNCG 		5' ---GCNG   C--- 3'
3' ---C   GNCG--- 5'
BthDI Bacillus thuringiensis D4 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AorI, ApyI, Bse24I, BstNI, BstOI, Bst2UI, CbrI, EcoRII, MvaI, SleI
BthEI Bacillus thuringiensis E 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI
BtkI Bacillus thuringiensis Kumantoensis 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		BepI, Bpu95I, Bsp123I, BstFNI, BstUI, BtkI, FalII, SelI, ThaI
BtkII Bacillus thuringiensis Kumantoensis 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BfuCI, Bsp67I, Bst19II, CcyI, FnuCI, MboI, NmeCI, SauMI
BtrI Bacillus stearothermophilus SE-U62 5' CACGTC
3' GTGCAG 		5' ---CAC   GTC--- 3'
3' ---GTG   CAG--- 5'
BtsI Bacillus thermoglucosidasius 5' GCAGTG
3' CGTCAC 		5' ---GCAGTGNN   --- 3'
3' ---CGTCAC   NN--- 5'
BveI Brevundimonas vesicularis RFL1 5' ACCTGC
3' TGGACG 		5' ---ACCTGCNNNN   NNNN--- 3'
3' ---TGGACGNNNNNNNN   --- 5' 		Acc36I, BfuAI, BspMI
BvuI Bacillus vulgatis 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
BvuBI Bacteroides vulgatus 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
This list is incomplete; you can help by adding missing items with reliable sources.

Notes
  1. Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
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