Neutrophil cytosolic factor 2

Neutrophil cytosol factor 2 is a protein that in humans is encoded by the NCF2 gene.

NCF2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1E96, 1HH8, 1K4U, 1OEY, 1WM5, 2DMO
Identifiers
Aliases	NCF2, NCF-2, NOXA2, P67-PHOX, P67PHOX, Neutrophil cytosolic factor 2
External IDs	OMIM: 608515 MGI: 97284 HomoloGene: 374 GeneCards: NCF2
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1q25.3 Start 183,555,563 bp[1] End 183,590,876 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1 G3|1 65.05 cM Start 152,800,194 bp[2] End 152,836,991 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • Rac GTPase binding • superoxide-generating NADPH oxidase activity • protein C-terminus binding • GO:0001948 protein binding • electron transfer activity • superoxide-generating NADPH oxidase activator activity Cellular component • Acrosome • nucleolus • phagolysosome • cytoplasm • cytosol • NADPH oxidase complex Biological process • superoxide anion generation • cellular defense response • vascular endothelial growth factor receptor signaling pathway • innate immune system • oxidation-reduction process • superoxide metabolic process • phagocytosis • GO:0048554 positive regulation of catalytic activity • respiratory burst • electron transport chain • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent • cellular response to oxidative stress • cell redox homeostasis Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4688	17970
Ensembl	ENSG00000116701	ENSMUSG00000026480
UniProt	P19878	O70145
RefSeq (mRNA)	NM_000433 NM_001127651 NM_001190789 NM_001190794	NM_010877
RefSeq (protein)	NP_000424 NP_001121123 NP_001177718 NP_001177723	NP_035007
Location (UCSC)	Chr 1: 183.56 – 183.59 Mb	Chr 1: 152.8 – 152.84 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.[5]

References

1. GRCh38: Ensembl release 89: ENSG00000116701 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026480 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: NCF2 neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2)".

Further reading

• Wientjes FB, Segal AW (December 1995). "NADPH oxidase and the respiratory burst". Seminars in Cell Biology. 6 (6): 357–65. doi:10.1016/S1043-4682(05)80006-6. PMID 8748143.
• DeLeo FR, Quinn MT (December 1996). "Assembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteins". Journal of Leukocyte Biology. 60 (6): 677–91. doi:10.1002/jlb.60.6.677. PMID 8975869. S2CID 17946755.
• Dorseuil O, Gacon G (1997). "[Signal transduction by Rac small G proteins in phagocytes]". Comptes Rendus des Séances de la Société de Biologie et de ses Filiales. 191 (2): 237–46. PMID 9255350.
• Leto TL, Lomax KJ, Volpp BD, Nunoi H, Sechler JM, Nauseef WM, Clark RA, Gallin JI, Malech HL (May 1990). "Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src". Science. 248 (4956): 727–30. Bibcode:1990Sci...248..727L. doi:10.1126/science.1692159. PMID 1692159.
• Kenney RT, Leto TL (December 1990). "A HindIII polymorphism in the human NCF2 gene". Nucleic Acids Research. 18 (23): 7193. doi:10.1093/nar/18.23.7193-a. PMC 332837. PMID 1979859.
• Francke U, Hsieh CL, Foellmer BE, Lomax KJ, Malech HL, Leto TL (September 1990). "Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1)". American Journal of Human Genetics. 47 (3): 483–92. PMC 1683885. PMID 2393022.
• Kenney RT, Malech HL, Epstein ND, Roberts RL, Leto TL (December 1993). "Characterization of the p67phox gene: genomic organization and restriction fragment length polymorphism analysis for prenatal diagnosis in chronic granulomatous disease". Blood. 82 (12): 3739–44. doi:10.1182/blood.V82.12.3739.3739. PMID 7903171.
• Leto TL, Adams AG, de Mendez I (October 1994). "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets". Proceedings of the National Academy of Sciences of the United States of America. 91 (22): 10650–4. Bibcode:1994PNAS...9110650L. doi:10.1073/pnas.91.22.10650. PMC 45079. PMID 7938008.
• Dusi S, Rossi F (December 1993). "Activation of NADPH oxidase of human neutrophils involves the phosphorylation and the translocation of cytosolic p67phox". The Biochemical Journal. 296 ( Pt 2) (2): 367–71. doi:10.1042/bj2960367. PMC 1137705. PMID 8257426.
• de Boer M, Hilarius-Stokman PM, Hossle JP, Verhoeven AJ, Graf N, Kenney RT, Seger R, Roos D (January 1994). "Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers". Blood. 83 (2): 531–6. doi:10.1182/blood.V83.2.531.531. PMID 8286749.
• Bonaldo MF, Lennon G, Soares MB (September 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Bonizzato A, Russo MP, Donini M, Dusi S (February 1997). "Identification of a double mutation (D160V-K161E) in the p67phox gene of a chronic granulomatous disease patient". Biochemical and Biophysical Research Communications. 231 (3): 861–3. doi:10.1006/bbrc.1997.6204. PMID 9070911.
• Sathyamoorthy M, de Mendez I, Adams AG, Leto TL (April 1997). "p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain". The Journal of Biological Chemistry. 272 (14): 9141–6. doi:10.1074/jbc.272.14.9141. PMID 9083043.
• Ahmed S, Prigmore E, Govind S, Veryard C, Kozma R, Wientjes FB, Segal AW, Lim L (June 1998). "Cryptic Rac-binding and p21(Cdc42Hs/Rac)-activated kinase phosphorylation sites of NADPH oxidase component p67(phox)". The Journal of Biological Chemistry. 273 (25): 15693–701. doi:10.1074/jbc.273.25.15693. PMID 9624165.
• Faris SL, Rinckel LA, Huang J, Hong YR, Kleinberg ME (June 1998). "Phagocyte NADPH oxidase p67-phox possesses a novel carboxylterminal binding site for the GTPases Rac2 and Cdc42". Biochemical and Biophysical Research Communications. 247 (2): 271–6. doi:10.1006/bbrc.1998.8775. PMID 9642115.
• Rinckel LA, Faris SL, Hitt ND, Kleinberg ME (September 1999). "Rac1 disrupts p67phox/p40phox binding: a novel role for Rac in NADPH oxidase activation". Biochemical and Biophysical Research Communications. 263 (1): 118–22. doi:10.1006/bbrc.1999.1334. PMID 10486263.
• Patiño PJ, Rae J, Noack D, Erickson R, Ding J, de Olarte DG, Curnutte JT (October 1999). "Molecular characterization of autosomal recessive chronic granulomatous disease caused by a defect of the nicotinamide adenine dinucleotide phosphate (reduced form) oxidase component p67-phox". Blood. 94 (7): 2505–14. doi:10.1182/blood.V94.7.2505.419k10_2505_2514. PMID 10498624.

External links

• Overview of all the structural information available in the PDB for UniProt: P19878 (Neutrophil cytosol factor 2) at the PDBe-KB.