PGM1

Phosphoglucomutase-1 is an enzyme that in humans is encoded by the PGM1 gene.[5][6][7] The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red blood cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause CDG syndrome type 1t (CDG1T, formerly known as glycogen storage disease type XIV). Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010][7]

PGM1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesPGM1, CDG1T, GSD14, phosphoglucomutase 1
External IDsOMIM: 171900 MGI: 97565 HomoloGene: 1979 GeneCards: PGM1
Gene location (Human)
Chr.Chromosome 1 (human)[1]
Band1p31.3Start63,593,411 bp[1]
End63,660,245 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

5236

72157

Ensembl

ENSG00000079739

ENSMUSG00000025791

UniProt

P36871

Q9D0F9

RefSeq (mRNA)

NM_002633
NM_001172818
NM_001172819

NM_028132

RefSeq (protein)

NP_001166289
NP_001166290
NP_002624

NP_082408

Location (UCSC)Chr 1: 63.59 – 63.66 MbChr 4: 99.93 – 99.99 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Structure

The PGM1 gene is localized to the first chromosome, with its specific region being 1p31.[8] The complete PGM1 gene spans over 65 kb and contains 11 exons, and the sites of the two mutations which form the molecular basis for the common PGM1 protein polymorphism lie in exons 4 and 8 and are 18 kb apart. Within this region there is a site of intragenic recombination. There are two alternatively spliced first exons, one of which, exon 1A, is transcribed in a wide variety of cell types; the other, exon 1B, is transcribed in fast muscle tissue. Exon 1A is transcribed from a promoter that has the structural hallmarks of a housekeeping promoter but lies more than 35 kb upstream of exon 2. Exon 1B lies 6 kb upstream of exon 2 within the large first intron of the ubiquitously expressed PGM1 transcript. The fast-muscle form of PGM1 is characterized by 18 extra amino acid residues at its N-terminal end. Sequence comparisons show that exons 1A and 1B are structurally related and have arisen by duplication. [9]

PGM1 is a monomeric protein with 562 amino acids and four structural domains arranged in an overall heart shape. The active site is located in the large, centrally located cleft, formed by more than 80 residues. The active site can be segregated into four highly conserved regions that contribute to catalysis and substrate binding.[10] These regions are: the phosphoserine residue that participates in phosphoryl transfer; the metal- binding loop; a sugar-binding loop; and the phosphate-binding site that interacts with the phosphate group of the substrate.[11] The active site cleft of PGM1 relies on all four structural domains of the enzyme for its structural integrity.[12][13]

Function

The biochemical pathways required to utilize glucose as a carbon and energy source are highly conserved from bacteria to humans. PGM1 is an evolutionarily conserved enzyme that regulates one of the most important metabolic carbohydrate trafficking points in prokaryotic and eukaryotic organisms, catalyzing the bi-directional interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P). In one direction, G-1-P produced from sucrose catabolism is converted to G-6-P, the first intermediate in glycolysis. In the other direction, conversion of G-6-P to G-1-P generates a substrate for synthesis of UDP-glucose, which is required for synthesis of a variety of cellular constituents, including cell wall polymers and glycoproteins.[14] PGM1 has been used extensively as a genetic marker for isozyme polymorphism among humans. PGM is known to be post-translationally modified by cytoplasmic glycosylation that does not seem to regulate its enzymatic activity but rather is implicated in the localization of the protein.[15] Glucose 1,6 bisphosphate (Glc-1, 6-P2), a powerful regulator of carbohydrate metabolism, has been demonstrated to be a potent activator of PGM. PGM1 is also modified by phosphorylation on Ser108 as part of its catalytic mechanism. This is shown to be performed by Pak1, a previously identified signaling kinase.[16]

Clinical significance

Phosphoglucomutase 1 (PGM1) deficiency is an inherited metabolic disorder in humans (CDG syndrome type 1t, CDG1T). Affected patients show multiple disease phenotypes, including dilated cardiomyopathy, exercise intolerance, and hepatopathy, reflecting the central role of the enzyme in glucose metabolism. The biochemical phenotypes of the PGM1 mutants cluster into two groups: those with compromised catalysis and those with possible folding defects. Relative to the recombinant wild-type enzyme, certain missense mutants show greatly decreased expression of soluble protein and/or increased aggregation. In contrast, other missense variants are well behaved in solution, but show dramatic reductions in enzyme activity, with Kcat/Km often <1.5% of wild-type. Modest changes in protein conformation and flexibility are also apparent in some of the catalytically impaired variants. In the case of the G291R mutant, severely compromised activity is linked to the inability of a key active site serine to be phosphorylated, a prerequisite for catalysis. Our results complement previous in vivo studies, which suggest that both protein misfolding and catalytic impairment may play a role in PGM1 deficiency.[17]

Interactions

PGM1 has been shown to interact with S100 calcium binding protein A1[18] and S100B.[18]

References
  1. GRCh38: Ensembl release 89: ENSG00000079739 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000025791 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Douglas GR, McAlpine PJ, Hamerton JL (Oct 1973). "Regional localization of loci for human PGM and 6PGD on human chromosome one by use of hybrids of Chinese hamster-human somatic cells". Proceedings of the National Academy of Sciences of the United States of America. 70 (10): 2737–40. doi:10.1073/pnas.70.10.2737. PMC 427098. PMID 4517931.
  6. Whitehouse DB, Putt W, Lovegrove JU, Morrison K, Hollyoake M, Fox MF, Hopkinson DA, Edwards YH (Jan 1992). "Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1". Proceedings of the National Academy of Sciences of the United States of America. 89 (1): 411–5. doi:10.1073/pnas.89.1.411. PMC 48247. PMID 1530890.
  7. "Entrez Gene: PGM1 phosphoglucomutase 1".
  8. Douglas GR, McAlpine PJ, Hamerton JL (Oct 1973). "Regional localization of loci for human PGM and 6PGD on human chromosome one by use of hybrids of Chinese hamster-human somatic cells". Proceedings of the National Academy of Sciences of the United States of America. 70 (10): 2737–40. doi:10.1073/pnas.70.10.2737. PMC 427098. PMID 4517931.
  9. Putt W, Ives JH, Hollyoake M, Hopkinson DA, Whitehouse DB, Edwards YH (Dec 1993). "Phosphoglucomutase 1: a gene with two promoters and a duplicated first exon". The Biochemical Journal. 296 (2): 417–22. doi:10.1042/bj2960417. PMC 1137712. PMID 8257433.
  10. Shackelford GS, Regni CA, Beamer LJ (Aug 2004). "Evolutionary trace analysis of the alpha-D-phosphohexomutase superfamily". Protein Science. 13 (8): 2130–8. doi:10.1110/ps.04801104. PMC 2279825. PMID 15238632.
  11. Liu Y, Ray WJ, Baranidharan S (Jul 1997). "Structure of rabbit muscle phosphoglucomutase refined at 2.4 A resolution". Acta Crystallographica Section D. 53 (Pt 4): 392–405. doi:10.1107/S0907444997000875. PMID 15299905.
  12. Beamer LJ (Mar 2015). "Mutations in hereditary phosphoglucomutase 1 deficiency map to key regions of enzyme structure and function". Journal of Inherited Metabolic Disease. 38 (2): 243–56. doi:10.1007/s10545-014-9757-9. PMID 25168163.
  13. Luebbering EK, Mick J, Singh RK, Tanner JJ, Mehra-Chaudhary R, Beamer LJ (Nov 2012). "Conservation of functionally important global motions in an enzyme superfamily across varying quaternary structures". Journal of Molecular Biology. 423 (5): 831–46. doi:10.1016/j.jmb.2012.08.013. PMID 22935436.
  14. Boros LG, Lee WN, Go VL (Jan 2002). "A metabolic hypothesis of cell growth and death in pancreatic cancer". Pancreas. 24 (1): 26–33. CiteSeerX 10.1.1.537.3798. doi:10.1097/00006676-200201000-00004. PMID 11741179.
  15. Dey NB, Bounelis P, Fritz TA, Bedwell DM, Marchase RB (Oct 1994). "The glycosylation of phosphoglucomutase is modulated by carbon source and heat shock in Saccharomyces cerevisiae". The Journal of Biological Chemistry. 269 (43): 27143–8. PMID 7929458.
  16. Gururaj A, Barnes CJ, Vadlamudi RK, Kumar R (Oct 2004). "Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase". Oncogene. 23 (49): 8118–27. doi:10.1038/sj.onc.1207969. PMID 15378030.
  17. Lee Y, Stiers KM, Kain BN, Beamer LJ (Nov 2014). "Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency". The Journal of Biological Chemistry. 289 (46): 32010–9. doi:10.1074/jbc.M114.597914. PMC 4231678. PMID 25288802.
  18. Landar A, Caddell G, Chessher J, Zimmer DB (Sep 1996). "Identification of an S100A1/S100B target protein: phosphoglucomutase". Cell Calcium. 20 (3): 279–85. doi:10.1016/S0143-4160(96)90033-0. PMID 8894274.

Further reading
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