Podospora anserina

Podospora anserina is a model filamentous ascomycete fungus. It is pseudohomothallic and non-pathogenic to humans.[1] This species is coprophilous, colonising the dung of herbivorous animals.[2]

Podospora anserina
Wild-type strain on a Petri dish
Scientific classification
Kingdom:
Fungi
Division:
Ascomycota
Subdivision:
Pezizomycotina
Class:
Sordariomycetes
Order:
Sordariales
Family:
Lasiosphaeriaceae
Genus:
Podospora
Species:
P. anserina
Binomial name
Podospora anserina
(Rabenh.) Niessl, 1883
Synonyms
  • Malinvernia anserina Rabenh., 1857
  • Sordaria anserina (Rabenh.) G. Winter, 1873
  • Pleurage anserina (Rabenh.) Kuntze, 1898

Taxonomy

Podospora anserina was originally named Malinvernia anserina Rabenhorst (1857) and Podospora anserina was subsequently published in Niessl von Mayendorf, G. 1883: Ueber die Theilung der Gattung Sordaria. Hedwigia 22: 153–156, which is used today to reference the common laboratory strain therefrom, namely, 'Niessl'. It is also known as Pleurage anserina (Ces.) Kuntze.[3][4] Genetics of P. anserina were characterized in Rizet and Engelmann (1949) and reviewed by Esser (1974). P. anserina is estimated to have diverged from N. crassa 75 million years ago based on 18s rRNA and protein orthologous share 60-70% homology.[5] NCBI Taxonomy ID: 5145,[6] MycoBank # 100818[7] Gene cluster orthologs between Aspergillus nidulans and Podospora anserina have 63% identical primary amino acid sequence (even those these species are from distinct classes) and the average amino acid of compared proteomes is 10% less, giving rise to hypotheses of distinct species yet shared genes

Research

Podospora as a model organism to study genetics, aging (senescence, cell degeneration), ascomycete development, heterokaryon incompatibility (mating in fungi),[8] prions, and mitochondrial and peroxisomal physiology.[9] Podospora is easily culturable (for example, on/in complex (full) potato dextrose or cornmeal agar/broth or even synthetic medium), and, using modern molecular tools, is easy to manipulate. Its optimal growth temperature is 25-27 °C.

Strains

Morphology varies depending on the specific strain.

  • ΔPaKu70 is used to increase homologous recombination in protoplasts during transformations in order to create desirable gene deletions or allelic mutations. A ΔPaKu70 strain can be achieved by transforming protoplasts with linear DNA that flanks the PaKu70 gene along with an antibiotic cassette and then selecting for strains and verifying by PCR.
  • Mn19 is a long-lived strain used to study senescence. It is derived from strain A+-84-11 after grown on manganese (Mn). This particular strain has been reported to have lived over 2 years in a race tube covering over 400 cm of vegetative growth.[10]
  • ΔiΔviv is an immortal strain that shows no sign of senescence. It produces yellow pigmentation. Lack of viv increased life span in days by a factor of 2.3 compared to the wild type and lack of i by 1.6, however, strain ΔiΔviv showed no senescence during the whole study and was vegetative for over a year. These genes are synergistic and are physically closely linked.[11]
  • AL2 is a long-lived strain. Insertion of linear mitochondrial plasmid containing al-2 show increased life span. However, natural isolates that have homology to al-2 do not show increased life span.[12]
  • Δgrisea is a long-lived strain and copper uptake mutant. This strain has lower affinity to copper and thus lower intracellular copper levels, leading to use of the cyanide-resistant alternative oxidase, PaAOX, pathway (instead of copper-dependent mitochondrial COX complex). This strain also exhibits more stable mtDNA. Copper use is similar to Δex1 strain.[13]
  • Δex1 is an 'immortal strain' that has been grown for over 12 years and still shows no signs of senescence. This strain respires via a cyanide-resistant, SHAM-sensitive pathway. This deletion disrupts the COX complex[14]
  • wild-type s strain is a wild type strain used in many studies. Described in Esser, 1974.

Aging

Podospora anserina has a definite life span and shows senescence phenotypically (by slower growth, less aerial hyphae, and increased pigment production in distal hyphae). However, isolates show either increased life span or immortality. To study the process of aging many genetic manipulations to produce immortal strains or increase life-span have been done. In general, the mitochondrion and mitochondrial chromosome is investigated (note that animals, closely related to fungi, contain similar organelles like mitochondria). This is because during respiration reactive oxygen species are produced that limit the life span and over time defective mitochondrial DNA can accumulate.[15][16] With this knowledge, much focus turned to nutrition availability, respiration (ATP synthesis) and oxidases, like cytochrome c oxidase. Carotenoids, pigments also found in plants and provide health benefits to humans,[17] are known to be in fungi like Podospora's divergent ancestor Neurospora crassa. In N. crassa (and other fungi) cartenoids al genes, namely focused provide UV radiation protection. Overexpressed of al-2 Podospora anserina increased life span by 31%.[18] Calorie restriction studies show that decreased nutrition, like sugar, increase life span (likely due to slower metabolism and thus decreased reactive oxygen species production or induced survival genes). Also, intracellular copper levels were found to be correlated with growth. This was studied in Grisea-deleted and ex1-deleted strains, as well as in a wild type s strain. Podospora without Grisea, a cooper transcription factor, had decreased intracellular copper levels which lead to use of an alternative respiratory pathway that consequently produced less oxidative stress.[19]

Heterokaryon incompatibility

The following genes, both allelic and nonallelic, are found to be involved in vegetative incompatibility (only those cloned and characterized are listed): het-c, het-c, het-s, idi-2, idi-1, idi-3, mod-A, mode-D, mod-E, psp-A. Podospora anserina contains at least 9 het loci.[20]

Enzymes

Podospora anserina is known to produce laccases, a type of phenoloxidase.[21]

Genetics

Original genetic studies by gel electrophoresis led to the finding of the genome size, ca. 35 megabases, with 7 chromosomes and 1 mitochondrial chromosome. In the 1980s the mitochondrial chromosome was sequenced. Then in 2003 a pilot study was initiated to sequence regions bordering chromosome V's centromere using BAC clones and direct sequencing.[22] In 2008, a 10x whole genome draft sequence was published.[23] The genome size is now estimated to be 35-36 megabases.[24] Genetic manipulation in fungi is difficult due to low homologous recombination efficiency and ectopic integrations (insertion of gene at undesirable location)[25] and thus a hindrance in genetic studies (allele replacement and knockouts).[26] Although in 2005, a method for gene deletion (knock-outs) was developed based on a model for Aspergillus nidulans that involved cosmid plasmid transformation, a better system for Podospora was developed in 2008 by using a strain that lacked nonhomologous end joining proteins (Ku (protein), known in Podospora as PaKu70). This method claimed to have 100% of transformants undergo desired homologous recombination leading to allelic replacement (after the transformation, the PaKu70 deletion can be restored by crossing over with a wild-type strain to yield progeny with only the targeted gene deletion or allelic exchange (e.g. point mutation)).[27]

Secondary metabolites

It is well known that many organisms across all domains produce secondary metabolites. Fungi are known to be prolific in this regard. Product mining was well underway in the 1990s for the genus Podospora. Specifically for Podospora anserina, two new natural products classified as pentaketides, specifically derivatives of benzoquinones, were discovered; these showed antifungal, antibacterial, and cytotoxic activities.[28] Horizontal gene transfer is common in bacteria and between prokaryotes and eukaryotes yet is more rare between eukaryotic organisms. Between fungi, secondary metabolite clusters are good candidates for HGT. For example, a functional ST gene cluster that produces sterigmatocystin was found in Podospora anserina and originally derived from Aspergillus. This cluster is well-conserved, notably the transcription-factor binding sites. Sterigmatocystin itself is toxic and is a precursor to another toxic metabolite, aflatoxin.[29]

See also

References
  1. "Podospora anserina: a model filamentous fungus"
  2. Bills GF, Gloer JB, An Z (October 2013). "Coprophilous fungi: antibiotic discovery and functions in an underexplored arena of microbial defensive mutualism". Current Opinion in Microbiology. 16 (5): 549–565. doi:10.1016/j.mib.2013.08.001. PMID 23978412.
  3. Memoirs of the Torrey Botanical Club (1902)
  4. "Podospora anserina".
  5. The genome sequence of the model ascomycete fungus Podospora anserina
  6. "Taxonomy Browser".
  7. "Podospora anserina".
  8. The transcriptional response to nonself in the fungus Podospora anserina.
  9. Gene deletion and allelic replacement in the Wlamentous fungus Podospora anserina
  10. Genetic and molecular analysis of a long-lived strain of Podospora anserina.
  11. Genes Inhibiting Senescence in the Ascomycete Podospora anserina
  12. The mitochondrial plasmid pAL2-1 reduces calorie restriction mediated life span extension in the filamentous fungus Podospora anserina 2004
  13. Copper-Modulated Gene Expression and Senescence in the Filamentous Fungus Podospora anserina
  14. Copper-Modulated Gene Expression and Senescence in the Filamentous Fungus Podospora anserina
  15. The mitochondrial plasmid pAL2-1 reduces calorie restriction mediated life span extension in the filamentous fungus Podospora anserina 2004
  16. GENETIC DISSECTION OF COMPLEX BIOLOGICAL TRAITS; THE LIFESPAN EXTENDING EFFECT OF CALORIE RESTRICTION IN THE FILAMENTOUS FUNGUS PODOSPORA ANSERINA
  17. The role of carotenoids in human health
  18. Carotenoids and carotenogenic genes in Podospora anserina: engineering of the carotenoid composition extends the life span of the mycelium
  19. Copper-Modulated Gene Expression and Senescence in the Filamentous Fungus Podospora anserina
  20. David Moore. Essential Fungal Genetics. page 40
  21. THE PHENOLOXIDASES * OF THE ASCOMYCETE PODOSPORA ANSERZNA. COMMUNICATION VI. GENETIC REGULATION OF THE FORMATION OF LACCASE
  22. Characterization of the genomic organization of the region bordering the centromere of chromosome V of Podospora anserina by direct sequencing.
  23. The genome sequence of the model ascomycete fungus Podospora anserina
  24. The genome sequence of the model ascomycete fungus Podospora anserina
  25. Relationship of vector insert size to homologous integration during transformation of Neurospora crassa with the cloned am (GDH) gene
  26. Gene deletion and allelic replacement in the Wlamentous fungus Podospora anserina
  27. Gene deletion and allelic replacement in the Wlamentous fungus Podospora anserina
  28. Anserinones A and B: new antifungal and antibacterial benzoquinones from the coprophilous fungus Podospora anserina.
  29. Slot, Jason C.; Rokas, Antonis (2011). "Horizontal Transfer of a Large and Highly Toxic Secondary Metabolic Gene Cluster between Fungi". Current Biology. 21 (2): 134–139. doi:10.1016/j.cub.2010.12.020. PMID 21194949.
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