Rhodobacter capsulatus

Rhodobacter capsulatus is a species of purple bacteria, a group of bacteria that can obtain energy through photosynthesis. Its name is derived from the Latin adjective "capsulatus" ("with a chest", "encapsulated"), itself derived Latin noun "capsula" (meaning "a small box or chest"), and the associated Latin suffix for masculine nouns, "-atus" (denoting that something is "provided with" something else).[4]

Rhodobacter capsulatus
Scientific classification
Domain:
Bacteria
Phylum:
Proteobacteria
Class:
Alphaproteobacteria
Order:
Rhodobacterales
Family:
Rhodobacteraceae
Genus:
Rhodobacter
Species:
R. capsulatus
Binomial name
Rhodobacter capsulatus
(Molisch 1907) Imhoff et al. 1984[1]
Type strain
ATCC 11166, ATCC 17015, ATH 2.3.1, BCRC 16406, C10, CCRC 16406, CCTM 1913, CCUG 31484, CGMCC 1.2359, CGMCC 1.3366, CIP 104408, DSM 1710, Ewart C10, HMSATH.2.3.1, IAM 14232, IFO 16435, JCM 21090, KCTC 2583, LMG 2962, NBRC 16435, NCIB 8254, NCIMB 8254, van Niel ATH.2.3.1, van Niel ATH.2.3.1.[2]
Synonyms[3]

Rhodopseudomonas capsulata

Its complete genome has been sequenced[5] and is available to the public.[6]

Discovery

The discovery of Rhodobacter capsulatus is attributed to Hans Molisch, a Czech-Austrian botanist. The microorganism, then named Rhodonostoc capsulatum, was identified in 1907 in his book Die Purpurbakterien nach neuen Untersuchungen.[7] C. B. van Niel then characterized the species further in 1944 where it was renamed Rhodopseudomonas capulata. Van Niel initially described 16 strains of R. capsulata that he was able to culture from mud samples collected in California and Cuba.[8] In 1984, the species would be reclassified as Rhodobacter capsulatus with the introduction of the genus Rhodobacter. This genus was introduced to better differentiate Rhodopseudomonas species with distinct morphological differences such as those with vesicular intracytoplasmic membranes (membrane-bound compartments in the cell often involved in photosynthesis)[9] like R. capsulatus and R. sphaeroides.[1]

Genomic Characteristics

The R. capusulatus genome consists of one chromosome and one plasmid. Sanger sequencing was first used to assemble the genome. The complete genome was then analyzed using several programs, Critica, Glimmer, RNAmmer, tRNAscan, and ARAGORN. These programs all identify different groups of genes, including protein-coding, tRNA, tmRNA, and rRNA genes. The chromosome is approximately 3.7-Mb with 3,531 open reading frames (ORFs), while the plasmid is smaller at 133-kb and 154 ORFs. Within the 3,531 ORFs in the chromosome, 3,100 had a known function assigned. Another 610 ORFs had similarities to genes that are known, but their function is still not proven. The rest of the ORFs were novel, with nothing similar in UniRef90, NCBI-NR, COG, or KEGG databases used for comparison. The genetic material had a high GC content at 66.6%. R. capsulatus contains all of the genes necessary to produce all 20 amino acids, and also contains 42 transposase genes, and 237 phage genes, including the gene transfer agent (GTA). The chromosome can be found in the NCBI database under CP001312, and the plasmid is under accession number CP001313.[10]

Ecology

These bacteria prefer aqueous environments[7] such as those around natural water sources or in sewage.[11] R. capsulatus has been isolated from the United States and Cuba.[12] Initially, this bacteria could be grown in the lab by plating samples from the environment onto RCVBN (DL-malic acid, ammonium sulfate, biotin, nicotonic acid, trace elements, and some additional compounds) medium and incubating them anaerobically with ample light. Colonies on these plates could then be isolated, grown in pure culture, and identified as R. capsulatus.[11] With the sequencing of its genome, RNA and DNA sequencing can now be used to identify this species.[6][13]

Morphology and Physiological Characteristics

R. capsulatus is a phototrophic bacterium with some distinctive characteristics. They can grow either as rods or as motile coccobacilli, which is dependent on their environment. At pH levels below 7, the bacterium is spherical and forms chains. When the pH rises above 7, they switch to rod morphology. The length of the rod shaped bacteria is dependent on the pH as well; the cells elongate as the pH rises. In their rod shape, they also often form chains that are bent in nature. The original paper describes them as "zigzaggy" in shape.[14] In response to the stress put on the cell at a pH of 8 or above, the cells display pleiomorphism, or abnormal, filamentous growth, and they produce a slimy substance for protection. Anaerobic culturing of the organism produces a brown color, on the spectrum of yellow-brown to burgundy. In media containing malonate, the reddish-brown, or burgundy, color is observed. When the organism is grown aerobically, a red color is produced. This species will not grow above 30 °C, and it will grow within 6 and 8.5 pH, although specific temperature and pH optima are not explicitly stated in the characterization paper.[14] Although most Rhodobacter species are freshwater and have little salt tolerance, some strains of R. capsulatus appear to tolerate up to 0.3 M NaCl depending on their source of nitrogen.[15]

Metabolism

As a purple non-sulfur bacterium, it is capable of aerobic growth without light, or anaerobic growth with light present, as well as fermentation.[16] This species is also capable of fixing nitrogen.[17] For carbon sources, R. capsulatus can utilize glucose, fructose, alanine, glutamic acid, propionate, glutaric acid, and other organic acids. However, it cannot use mannitol, tartrate, citrate, gluconate, ethanol, sorbitol, mannose, and leucine, which is unique to R. capsulatus when compared to other species in the genus. The most successful enrichments of this species come from proprionate and organic acids.[14] Under photoheterotrophic conditions, R. capsulatus strain B10 is capable of using acetate as its sole carbon source, but the mechanisms of this have not been identified.[18] The strains studied do not hydrolyze gelatin.[14]

Significance

Rhodobacter capsulatus was the first microorganism observed to produce gene transfer agents. A gene transfer agent (GTA) is a phage-like particle that transfers small amounts of DNA from the producing cell’s chromosome to aid in horizontal gene transfer. The DNA packaged in the particles is also random; it does not contain all the genes needed for GTA production. While somewhat similar to a transducing particle, GTAs are not created by accident when a phage is packaging DNA into viral particles. The genes for GTAs and their regulation are controlled by the cell itself, not a phage.[19] These particles were first identified when researchers put several different antibiotic resistant strains of R. capsulatus in co-culture and observed doubly-resistant strains. This DNA exchange was still observed even when cell contact was eliminated and DNases were added which allowed them to rule out conjugation and transformation as the cause. A small filterable agent was soon identified as the source of this genetic exchange.[20] When a mutant strain that over-produced these agents was created, it was proven the particles were not being produced by a phage, but by R. capsulatus.[21] After the genes for GTA production were sequenced, more species were found to produce GTAs leading to Rhodobacter capsulatus’s gene transfer agent being abbreviated to RcGTA.[19] It has been suggested that harsh conditions may trigger the cell to begin producing GTAs which would allow genomic DNA to be shared and increase the overall genetic diversity of the population.[22]

References
  1. Imhoff JF, Trüper HG, Pfennig N (July 1984). "Rearrangement of the Species and Genera of the Phototrophic "Purple Nonsulfur Bacteria"". International Journal of Systematic Bacteriology. 34 (3): 340–343. doi:10.1099/00207713-34-3-340.
  2. "Rhodobacter capsulatus (taxon passport)". StrainInfo. Retrieved 8 December 2015.
  3. "Rhodobacter capsulatus (Rhodopseudomonas capsulata)". UniProt. Retrieved 8 December 2015.
  4. "Rhodobacter capsulatus - Taxonomy Browser". StrainInfo. Retrieved 8 December 2015.
  5. Strnad H, Lapidus A, Paces J, Ulbrich P, Vlcek C, Paces V, Haselkorn R (July 2010). "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003". Journal of Bacteriology. 192 (13): 3545–6. doi:10.1128/JB.00366-10. PMC 2897665. PMID 20418398.
  6. "KEGG GENOME: Rhodobacter capsulatus". Kyoto Encyclopedia of Genes and Genomes. Retrieved 8 December 2015.
  7. Molisch H (1907). Die Purpurbakterien nach neuen Untersuchungen.
  8. van Niel CB (March 1944). "The Culture, General Physiology, Morphology, and Classification of the Non-Sulfur Purple and Brown Bacteria". Bacteriological Reviews. 8 (1): 1–118. doi:10.1128/MMBR.8.1.1-118.1944. PMC 440875. PMID 16350090.
  9. LaSarre B, Kysela DT, Stein BD, Ducret A, Brun YV, McKinlay JB (July 2018). "Restricted Localization of Photosynthetic Intracytoplasmic Membranes (ICMs) in Multiple Genera of Purple Nonsulfur Bacteria". mBio. 9 (4). doi:10.1128/mbio.00780-18. PMC 6030561. PMID 29970460.
  10. Strnad, Hynek; Lapidus, Alla; Paces, Jan; Ulbrich, Pavel; Vlcek, Cestmir; Paces, Vaclav; Haselkorn, Robert (2010-07-01). "Complete Genome Sequence of the Photosynthetic Purple Nonsulfur Bacterium Rhodobacter capsulatus SB 1003". Journal of Bacteriology. 192 (13): 3545–3546. doi:10.1128/JB.00366-10. ISSN 0021-9193. PMID 20418398.
  11. Weaver PF, Wall JD, Gest H (November 1975). "Characterization of Rhodopseudomonas capsulata". Archives of Microbiology. 105 (3): 207–16. doi:10.1007/BF00447139. PMID 1103769.
  12. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC (2014). "The Family Rhodobacteraceae". The Prokaryotes. Springer Berlin Heidelberg. pp. 439–512. doi:10.1007/978-3-642-30197-1_377. ISBN 978-3-642-30196-4.
  13. Strnad H, Lapidus A, Paces J, Ulbrich P, Vlcek C, Paces V, Haselkorn R (July 2010). "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003". Journal of Bacteriology. 192 (13): 3545–6. doi:10.1128/JB.00366-10. PMID 20418398.
  14. van Niel, C. B. (1944). "THE CULTURE, GENERAL PHYSIOLOGY, MORPHOLOGY, AND CLASSIFICATION OF THE NON-SULFUR PURPLE AND BROWN BACTERIA". Bacteriological Reviews. 8 (1): 1–118. doi:10.1128/MMBR.8.1.1-118.1944. ISSN 0005-3678. PMC 440875. PMID 16350090.
  15. Igeno, M. I.; Moral, C. G. Del; Castillo, F.; Caballero, F. J. (1995-08-01). "Halotolerance of the Phototrophic Bacterium Rhodobacter capsulatus E1F1 Is Dependent on the Nitrogen Source". Applied and Environmental Microbiology. 61 (8): 2970–2975. doi:10.1128/AEM.61.8.2970-2975.1995. ISSN 0099-2240. PMID 16535098.
  16. Tichi, Mary A.; Tabita, F. Robert (2001-11-01). "Interactive Control of Rhodobacter capsulatus Redox-Balancing Systems during Phototrophic Metabolism". Journal of Bacteriology. 183 (21): 6344–6354. doi:10.1128/JB.183.21.6344-6354.2001. ISSN 0021-9193. PMID 11591679.
  17. "Rhodobacter capsulatus (ID 1096) - Genome - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2020-04-13.
  18. Petushkova, E. P.; Tsygankov, A. A. (2017-05-01). "Acetate metabolism in the purple non-sulfur bacterium Rhodobacter capsulatus". Biochemistry (Moscow). 82 (5): 587–605. doi:10.1134/S0006297917050078. ISSN 1608-3040. PMID 28601069.
  19. Lang AS, Zhaxybayeva O, Beatty JT (June 2012). "Gene transfer agents: phage-like elements of genetic exchange". Nature Reviews. Microbiology. 10 (7): 472–82. doi:10.1038/nrmicro2802. PMC 3626599. PMID 22683880.
  20. Marrs B (March 1974). "Genetic recombination in Rhodopseudomonas capsulata". Proceedings of the National Academy of Sciences of the United States of America. 71 (3): 971–3. Bibcode:1974PNAS...71..971M. doi:10.1073/pnas.71.3.971. PMC 388139. PMID 4522805.
  21. Yen HC, Hu NT, Marrs BL (June 1979). "Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata". Journal of Molecular Biology. 131 (2): 157–68. doi:10.1016/0022-2836(79)90071-8. PMID 490646.
  22. Lang AS, Beatty JT (January 2000). "Genetic analysis of a bacterial genetic exchange element: the gene transfer agent of Rhodobacter capsulatus". Proceedings of the National Academy of Sciences of the United States of America. 97 (2): 859–64. Bibcode:2000PNAS...97..859L. doi:10.1073/pnas.97.2.859. PMC 15421. PMID 10639170.
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