Rotylenchulus reniformis

Rotylenchulus reniformis, the reniform nematode, is a species of parasitic nematode of plants with a worldwide distribution in the tropical and subtropical regions.[1]

Rotylenchulus reniformis
Scientific classification
Kingdom:	Animalia
Phylum:	Nematoda
Class:	Secernentea
Order:	Tylenchida
Family:	Hoplolaimidae
Genus:	Rotylenchulus
Species:	R. reniformis
Binomial name
Rotylenchulus reniformis Linford and Oliveira, 1940

Taxonomy

This nematode has a wide host range, infecting many species of plants around the world. It was first observed on the roots of cowpea in Hawaii, and was described as new species and new genus in 1940.[2] Its specific epithet, reniformis, was inspired by the kidney shape of the adult female.[1] There are now ten species classified in the genus, but R. reniformis is the only species of major economic importance to agriculture.[2]

Distribution and host range

R. reniformis has been reported from thousands of localities in the Americas, Africa, Europe, Asia, and Australia.[2][3] It has a wide host range that includes fruit trees, lentil, cotton, pigeon pea, tea, tobacco, soybean, pineapple, banana, okra, coconut, cabbage, sweet potato, alfalfa, corn, asparagus, palm, cucumber, tomato, squash, cassava, radish, eggplant, guava, melon, and ginger.[2][4] Certain plants are considered to be non-hosts of the nematode, such as little barley, common barnyard grass, pangola grass, peppers, and some cultivars of black mustard, oat, spinach, and sugarcane.[4]

Morphology

The reniform nematode has esophageal glands overlapping the intestine and a short stylet. The dorsal esophageal gland orifice is located posterior to the stylet knobs.[4][5] The immature female is slender and may be spiral- or C-shaped in death.[6] It is about 0.3 to 0.5 millimeters long.[7] The mature female has a swollen, kidney-shaped body with a short tail, a short, thin stylet with rounded stylet knobs, a three-part esophagus, a long and narrow isthmus, and a well-developed metacarpus. The vulva is just behind the middle of the body.[5] The male is vermiform: with a wormlike appearance. It has a weak stylet, curved spicules, and a pointed tail. The esophagus is reduced.[5][6]

Life cycle

R. reniformis is sedentary semi-endoparasite on the roots of plants.[3] The female penetrates the root and remains in one position at a permanent feeding site with its posterior end projecting from the root.[3] The immature female is the infective agent, attacking the root and growing to maturity at its feeding site.[8] Males and juveniles live in the soil; males are not parasites and do not feed.[5] Under drought conditions the nematode can persist up to two years outside a host by entering an anhydrobiotic state.[3]

The life cycle is 17 to 29 days long.[4] The juvenile molts once while still inside the egg.[9] The eggs hatch in 8 to 10 days. The juvenile molts three times to reach the immature stage.[9] The immature female parasitizes the root for one to two weeks.[3] During this time the male deposits sperm, which the female stores until her gonads mature. The nematode can also reproduce via parthenogenesis, without fertilization.[3] Upon maturity the female exits the root and lays up to 200 eggs in a gelatinous matrix.[3]

Host-parasite relationship

When the immature female penetrates the root, a feeding tube forms from stylet secretions.[6] Nematode infestation can cause symptoms in the plant that resemble those of moisture and nutrient deficiencies. It causes hypertrophy in the pericycle cells of seedling roots and in the periderm cells of the roots of older plants.[10] Root growth slows and secondary root development is limited. Root necrosis has been observed in pineapple and banana. Shoot growth suppression and reduction of fruit quality has been observed in crops such as pineapple.[8] Infested plants can become stunted and chlorotic. Wilt disease can follow when opportunistic fungi such as Fusarium and Verticillium infect the plants, a process observed in cotton.[3][5] Secondary fungal infection can also cause root decay. Damage from R. reniformis is directly related to the number of nematodes present when the crop is planted. Variation among nematode populations, hosts, environmental conditions, and soil types may alter the threshold or economic injury level across the geographic distribution of the nematode.[3][9]

Management

Some plants are resistant to this nematode.[4] In susceptible taxa, chemical control is one of the most common management practices. Nematicides before and after planting can be effective.[3][9] Intercropping and crop rotation, especially with resistant or non-host plants, is used as cultural control to improve soil, increase antagonistic microorganisms, and reduce nematode populations. French marigold (Tagetes patula) and sunn hemp (Crotalaria juncea) are recommended for these uses.[3][8] The fungus Purpureocillium lilacinum has been used as an agent of biological pest control against the nematode in chickpea.[11]

References

1. Ferris, H. Rotylenchulus reniformis. Nemaplex. Department of Nematology. University of California, Davis. 2012.
2. Robinson, A. F., et al. 1997. Rotylenchulus species: identification, distribution, host ranges, and crop plant resistance. Nematropica 27(2), 127-80.
3. Wang, K. Reniform Nematode, Rotylenchulus reniformis Linford and Oliveira (Nematoda: Tylenchida: Tylenchoidea: Hoplolaimidae: Rotylenchulinae). EENY-210 (IN367). Entomology and Nematology. Florida Cooperative Extension Service. University of Florida IFAS. Published 2001, revised 2007.
4. MacGowan, J. B. The Reniform Nematode. Nematology Circular No. 32. Florida Department of Agriculture and Consumer Services. 1977.
5. Shurtleff, M. C. and C. W. Averre. Diagnosing Plant Diseases Caused by Nematodes. St. Paul, Minnesota: American Phytopathological Society Press. 2000. ISBN 0890542546
6. Dropkin, V. Introduction to Plant Nematology. John Wiley & Sons, Inc. 1980. 293p.
7. Reniform Nematode. Sweetpotato DiagNotes. Centre for Biological Information Technology. University of Queensland.
8. Luc, M., et al. (Eds.) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd Edition. CABI Publishing. 2005. pp 38, 39, 709-20.
9. Lawrence, G. W. and K. S. Reniform Nematodes. In: Kirkpatrick, T. L. and C. S. Rothrock. (Eds.) Compendium of Cotton Diseases. 2nd edition. St. Paul, Minnesota: The American Phytopathological Society Press. 2001. pp. 42–44.
10. Oteifa, B. A. (1970). The reniform nematode problem of Egyptian cotton production. Journal of Parasitology 56, 255.
11. Ashraf, M. S. and T. A. Khan. (2008). Biomanagement of reniform nematode, Rotylenchulus reniformis by fruit wastes and Paecilomyces lilacinus on chickpea. World Journal of Agricultural Sciences 4(4), 492-94.