Steroid sulfotransferase
In enzymology, a steroid sulfotransferase (EC 2.8.2.15) is an enzyme that catalyzes the chemical reaction
- 3'-phosphoadenylyl sulfate + a phenolic steroid adenosine 3',5'-bisphosphate + steroid O-sulfate
| steroid sulfotransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.8.2.15 | ||||||||
| CAS number | 9032-76-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and phenolic steroid, whereas its two products are adenosine 3',5'-bisphosphate and steroid O-sulfate.
This enzyme belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:phenolic-steroid sulfotransferase. This enzyme is also called steroid alcohol sulfotransferase. This enzyme participates in steroid metabolism.
Genes
Of 62 sulfotransferase genes in the human genome, 16 represent cytoplasmic sulfotransferases, and of these 16 cytoplasmic sulfotransferases, five have been found to act as steroid sulfotransferases.[1] These five sulfotransferase genes are SULT1A1, SULT1E1, and SULT2A1, as well as the two isoforms of SULT2B1, SULT2B1a and SULT2B1b.[1] Their substrate specificity is as follows:[1]
- SULT1A1: Estradiol (to estradiol sulfate)
- SULT1E1: DHEA (to DHEA sulfate); Estrone (to estrone sulfate); Estradiol (to estradiol sulfate)
- SULT2A1: DHEA (to DHEA sulfate); Androsterone (to androsterone sulfate); Pregnenolone (to pregnenolone sulfate)
- SULT2B1a: Pregnenolone (to pregnenolone sulfate)
- SULT2B1b: Cholesterol (to cholesterol sulfate)
Traditionally, steroid sulfotransferases have been named according to their preferred substrate, for instance estrogen sulfotransferase (SULT1E1) and DHEA sulfotransferase (SULT2A1).[1] However, cytosolic steroid sulfotransferases show broad substrate specificity, and SULT1E1 and SULT2A1 are not the only steroid sulfotransferases that sulfate estrogens and DHEA, respectively.[1]
See also
- Estrogen sulfotransferase
- Estrone sulfotransferase
- Steroid sulfatase
- Steroidogenic enzyme
References
- Mueller JW, Gilligan LC, Idkowiak J, Arlt W, Foster PA (2015). "The Regulation of Steroid Action by Sulfation and Desulfation". Endocr. Rev. 36 (5): 526–63. doi:10.1210/er.2015-1036. PMC 4591525. PMID 26213785.
- Adams JB, McDonald D (1980). "Enzymic synthesis of steroid sulphates. XIII. Isolation and properties of dehydroepiandrosterone sulphotransferase from human foetal adrenals". Biochim. Biophys. Acta. 615 (1): 275–8. doi:10.1016/0005-2744(80)90031-5. PMID 6932974.