UGT8

2-hydroxyacylsphingosine 1-beta-galactosyltransferase is an enzyme that in humans is encoded by the UGT8 gene.[5][6]

UGT8
Identifiers
Aliases	UGT8, CGT, UGT4, UDP glycosyltransferase 8, GalCer Synthase, Ceramide Galactosyltransferase
External IDs	OMIM: 601291 MGI: 109522 HomoloGene: 20715 GeneCards: UGT8
Gene location (Human) Chr. Chromosome 4 (human)[1] Band 4q26 Start 114,598,455 bp[1] End 114,678,224 bp[1]
Gene location (Mouse) Chr. Chromosome 3 (mouse)[2] Band 3|3 G1 Start 125,865,271 bp[2] End 125,938,619 bp[2]
Gene ontology Molecular function • transferase activity, transferring hexosyl groups • transferase activity, transferring glycosyl groups • glucuronosyltransferase activity • transferase activity • N-acylsphingosine galactosyltransferase activity • UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity • UDP-glycosyltransferase activity Cellular component • integral component of membrane • membrane • intracellular membrane-bounded organelle • cell membrane Biological process • lipid metabolism • neuron projection morphogenesis • galactosylceramide biosynthetic process • glycosphingolipid metabolic process • peripheral nervous system development • central nervous system development • paranodal junction assembly • metabolism • protein localization to paranode region of axon • cytoskeleton organization • sphingolipid metabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	7368	22239
Ensembl	ENSG00000174607	ENSMUSG00000032854
UniProt	Q16880	Q64676
RefSeq (mRNA)	NM_001128174 NM_003360 NM_001322112 NM_001322113 NM_001322114	NM_011674
RefSeq (protein)	NP_001121646 NP_001309041 NP_001309042 NP_001309043 NP_003351	NP_035804
Location (UCSC)	Chr 4: 114.6 – 114.68 Mb	Chr 3: 125.87 – 125.94 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000174607 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032854 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bosio A, Binczek E, Le Beau MM, Fernald AA, Stoffel W (Dec 1996). "The human gene CGT encoding the UDP-galactose ceramide galactosyl transferase (cerebroside synthase): cloning, characterization, and assignment to human chromosome 4, band q26". Genomics. 34 (1): 69–75. doi:10.1006/geno.1996.0242. PMID 8661025.
6. "Entrez Gene: UGT8 UDP glycosyltransferase 8 (UDP-galactose ceramide galactosyltransferase)".

Further reading

• Yahi N, Spitalnik SL, Stefano KA, De Micco P, Gonzalez-Scarano F, Fantini J (1994). "Interferon-gamma decreases cell surface expression of galactosyl ceramide, the receptor for HIV-1 GP120 on human colonic epithelial cells". Virology. 204 (2): 550–7. doi:10.1006/viro.1994.1568. PMID 7941321.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Bhat S, Mettus RV, Reddy EP, Ugen KE, Srikanthan V, Williams WV, Weiner DB (1993). "The galactosyl ceramide/sulfatide receptor binding region of HIV-1 gp120 maps to amino acids 206-275". AIDS Res. Hum. Retroviruses. 9 (2): 175–81. doi:10.1089/aid.1993.9.175. PMID 8457384.
• Kapitonov D, Yu RK (1997). "Cloning, characterization, and expression of human ceramide galactosyltransferase cDNA". Biochem. Biophys. Res. Commun. 232 (2): 449–53. doi:10.1006/bbrc.1997.6240. PMID 9125199.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Sprong H, Kruithof B, Leijendekker R, Slot JW, van Meer G, van der Sluijs P (1998). "UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum". J. Biol. Chem. 273 (40): 25880–8. doi:10.1074/jbc.273.40.25880. PMID 9748263.
• Iida A, Saito S, Sekine A, Mishima C, Kitamura Y, Kondo K, Harigae S, Osawa S, Nakamura Y (2002). "Catalog of 86 single-nucleotide polymorphisms (SNPs) in three uridine diphosphate glycosyltransferase genes: UGT2A1, UGT2B15, and UGT8". J. Hum. Genet. 47 (10): 505–10. doi:10.1007/s100380200075. PMID 12376738.
• Sprong H, Degroote S, Nilsson T, Kawakita M, Ishida N, van der Sluijs P, van Meer G (2003). "Association of the Golgi UDP-galactose transporter with UDP-galactose:ceramide galactosyltransferase allows UDP-galactose import in the endoplasmic reticulum". Mol. Biol. Cell. 14 (8): 3482–93. doi:10.1091/mbc.E03-03-0130. PMC 181583. PMID 12925779.
• Tencomnao T, Kapitonov D, Bieberich E, Yu RK (2004). "Transcriptional regulation of the human UDP-galactose:ceramide galactosyltransferase (hCGT) gene expression: functional role of GC-box and CRE". Glycoconj. J. 20 (5): 339–51. doi:10.1023/B:GLYC.0000033630.58533.16. PMID 15229398. S2CID 38983312.