URI1

Unconventional prefoldin RPB5 interactor, also called URI1, is a protein that in humans is encoded by the URI1 gene.[5][6][7]

URI1
Identifiers
Aliases	URI1, C19orf2, NNX3, PPP1R19, RMP, URI, URI1 prefoldin-like chaperone, prefoldin like chaperone, URI1 prefoldin like chaperone
External IDs	OMIM: 603494 MGI: 1342294 HomoloGene: 2813 GeneCards: URI1
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q12 Start 29,923,644 bp[1] End 30,016,612 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 B3 Start 37,959,992 bp[2] End 38,023,551 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001106 transcription corepressor activity • phosphatase inhibitor activity • chromatin binding • phosphoprotein binding • GO:0001948 protein binding • protein phosphatase inhibitor activity • RNA polymerase II core binding Cellular component • cytoplasm • cell projection • dendrite • DNA-directed RNA polymerase II, core complex • cell nucleus • nucleoplasm • mitochondrion • cytosol Biological process • cellular response to steroid hormone stimulus • regulation of transcription, DNA-templated • negative regulation of intrinsic apoptotic signaling pathway • regulation of transcription from RNA polymerase II promoter • negative regulation of phosphatase activity • cellular response to growth factor stimulus • response to virus • negative regulation of transcription from RNA polymerase II promoter • transcription, DNA-templated • regulation of cell growth • negative regulation of phosphoprotein phosphatase activity Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	8725	19777
Ensembl	ENSG00000105176	ENSMUSG00000030421
UniProt	O94763	Q3TLD5
RefSeq (mRNA)	NM_001252641 NM_003796 NM_134447	NM_011274
RefSeq (protein)	NP_001239570 NP_003787	NP_035404
Location (UCSC)	Chr 19: 29.92 – 30.02 Mb	Chr 7: 37.96 – 38.02 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene binds to RNA polymerase II subunit 5 (RPB5) and negatively modulates transcription through its binding to RPB5. The encoded protein seems to have inhibitory effects on various types of activated transcription, but it requires the RPB5-binding region. This protein acts as a corepressor. It is suggested that it may require signaling processes for its function or that it negatively modulates genes in the chromatin structure. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.[7]

Interactions

URI1 has been shown to interact with DMAP1[8] and STAP1.[9]

Model organisms

Model organisms have been used in the study of URI1 function. A conditional knockout mouse line called Uri1^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.[10] Male and female animals underwent a standardized phenotypic screen[11] to determine the effects of deletion.[12][13][14][15] Additional screens performed: - In-depth immunological phenotyping[16]

Uri1 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[11][16]
Peripheral blood leukocytes 6 Weeks	Abnormal
Haematology 6 Weeks	Normal
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal

References

1. GRCh38: Ensembl release 89: ENSG00000105176 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030421 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Van Leuven F, Torrekens S, Moechars D, Hilliker C, Buellens M, Bollen M, Delabie J (Feb 1999). "Molecular cloning of a gene on chromosome 19q12 coding for a novel intracellular protein: analysis of expression in human and mouse tissues and in human tumor cells, particularly Reed-Sternberg cells in Hodgkin disease". Genomics. 54 (3): 511–20. doi:10.1006/geno.1998.5609. PMID 9878255.
6. Dorjsuren D, Lin Y, Wei W, Yamashita T, Nomura T, Hayashi N, Murakami S (Dec 1998). "RMP, a Novel RNA Polymerase II Subunit 5-Interacting Protein, Counteracts Transactivation by Hepatitis B Virus X Protein". Mol Cell Biol. 18 (12): 7546–55. doi:10.1128/mcb.18.12.7546. PMC 109335. PMID 9819440.
7. "Entrez Gene: C19orf2 chromosome 19 open reading frame 2".
8. Delgermaa L, Hayashi N, Dorjsuren D, Nomura T, Thuy le TT, Murakami S (Oct 2004). "Subcellular Localization of RPB5-Mediating Protein and Its Putative Functional Partner". Mol. Cell. Biol. 24 (19): 8556–66. CiteSeerX 10.1.1.605.6435. doi:10.1128/MCB.24.19.8556-8566.2004. PMC 516735. PMID 15367675.
9. Gstaiger M, Luke B, Hess D, Oakeley EJ, Wirbelauer C, Blondel M, Vigneron M, Peter M, Krek W (Nov 2003). "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI". Science. 302 (5648): 1208–12. Bibcode:2003Sci...302.1208G. doi:10.1126/science.1088401. PMID 14615539. S2CID 26836888.
10. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
11. "International Mouse Phenotyping Consortium".
12. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
13. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
14. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
15. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
16. "Infection and Immunity Immunophenotyping (3i) Consortium".

External links

• Human URI1 genome location and URI1 gene details page in the UCSC Genome Browser.

Further reading

• Liu L, McKeehan WL (2002). "Sequence analysis of LRPPRC and its SEC1 domain interaction partners suggests roles in cytoskeletal organization, vesicular trafficking, nucleocytosolic shuttling, and chromosome activity". Genomics. 79 (1): 124–36. doi:10.1006/geno.2001.6679. PMC 3241999. PMID 11827465.
• Wei W, Gu JX, Zhu CQ, Sun FY, Dorjsuren D, Lin Y, Murakami S (2003). "Interaction with general transcription factor IIF (TFIIF) is required for the suppression of activated transcription by RPB5-mediating protein (RMP)". Cell Res. 13 (2): 111–20. doi:10.1038/sj.cr.7290155. PMID 12737519.
• Gstaiger M, Luke B, Hess D, Oakeley EJ, Wirbelauer C, Blondel M, Vigneron M, Peter M, Krek W (2003). "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI". Science. 302 (5648): 1208–12. Bibcode:2003Sci...302.1208G. doi:10.1126/science.1088401. PMID 14615539. S2CID 26836888.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
• Delgermaa L, Hayashi N, Dorjsuren D, Nomura T, Thuy le TT, Murakami S (2004). "Subcellular Localization of RPB5-Mediating Protein and Its Putative Functional Partner". Mol. Cell. Biol. 24 (19): 8556–66. doi:10.1128/MCB.24.19.8556-8566.2004. PMC 516735. PMID 15367675.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.