RAD23A

UV excision repair protein RAD23 homolog A is a protein that in humans is encoded by the RAD23A gene.[5]

RAD23A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1DV0, 1F4I, 1IFY, 1OQY, 1P98, 1P9D, 1QZE, 1TP4, 2WYQ
Identifiers
Aliases	RAD23A, HHR23A, HR23A, RAD23 homolog A, nucleotide excision repair protein
External IDs	OMIM: 600061 MGI: 105126 HomoloGene: 48322 GeneCards: RAD23A
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19p13.13 Start 12,945,855 bp[1] End 12,953,642 bp[1]
Gene location (Mouse) Chr. Chromosome 8 (mouse)[2] Band 8|8 C2 Start 84,834,019 bp[2] End 84,840,665 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • single-stranded DNA binding • ubiquitin-specific protease binding • damaged DNA binding • polyubiquitin modification-dependent protein binding • GO:0001948 protein binding • kinase binding • ubiquitin binding • proteasome binding Cellular component • proteasome complex • nucleoplasm • microtubule organizing center • cytosol • cell nucleus • cytoplasm • intracellular membrane-bounded organelle • macromolecular complex Biological process • nucleotide-excision repair • positive regulation of viral genome replication • GO:0022415 viral process • DNA repair • cellular response to DNA damage stimulus • proteasome-mediated ubiquitin-dependent protein catabolic process • regulation of proteasomal ubiquitin-dependent protein catabolic process • protein deubiquitination • protein destabilization • positive regulation of proteasomal ubiquitin-dependent protein catabolic process • positive regulation of cell cycle Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5886	19358
Ensembl	ENSG00000179262	ENSMUSG00000003813
UniProt	P54725	P54726
RefSeq (mRNA)	NM_001270362 NM_001270363 NM_005053	NM_001297606 NM_001297607 NM_009010 NM_001378894 NM_001378895 NM_001378896
RefSeq (protein)	NP_001257291 NP_001257292 NP_005044	NP_001284535 NP_001284536 NP_033036 NP_001365823 NP_001365824 NP_001365825
Location (UCSC)	Chr 19: 12.95 – 12.95 Mb	Chr 8: 84.83 – 84.84 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.[6]

Interactions

RAD23A has been shown to interact with:

• Ataxin 3,[7]
• PSMD4,[8][9] and
• Sequestosome 1.[10]

References

1. GRCh38: Ensembl release 89: ENSG00000179262 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000003813 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. van der Spek PJ, Smit EM, Beverloo HB, Sugasawa K, Masutani C, Hanaoka F, Hoeijmakers JH, Hagemeijer A (March 1995). "Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23". Genomics. 23 (3): 651–658. doi:10.1006/geno.1994.1554. hdl:1765/3069. PMID 7851894.
6. "Entrez Gene: RAD23A RAD23 homolog A (S. cerevisiae)".
7. Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (July 2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–803. doi:10.1093/hmg/9.12.1795. PMID 10915768.
8. Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (September 1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–25. doi:10.1074/jbc.274.39.28019. PMID 10488153.
9. Mueller TD, Feigon J (September 2003). "Structural determinants for the binding of ubiquitin-like domains to the proteasome". EMBO J. 22 (18): 4634–45. doi:10.1093/emboj/cdg467. PMC 212733. PMID 12970176.
10. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

Further reading

• Eichbaum QG, Beatty DW, Parker MI (1994). "Identification of cardiac autoantigens in human heart cDNA libraries using acute rheumatic fever sera". J. Autoimmun. 7 (2): 243–261. doi:10.1006/jaut.1994.1019. PMID 8037842.
• Masutani C, Sugasawa K, Yanagisawa J, Sonoyama T, Ui M, Enomoto T, Takio K, Tanaka K, van der Spek PJ, Bootsma D (1994). "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23". EMBO J. 13 (8): 1831–43. doi:10.1002/j.1460-2075.1994.tb06452.x. PMC 395023. PMID 8168482.
• van der Spek PJ, Eker A, Rademakers S, Visser C, Sugasawa K, Masutani C, Hanaoka F, Bootsma D, Hoeijmakers JH (1996). "XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes". Nucleic Acids Res. 24 (13): 2551–2559. doi:10.1093/nar/24.13.2551. PMC 145966. PMID 8692695.
• van der Spek PJ, Visser CE, Hanaoka F, Smit B, Hagemeijer A, Bootsma D, Hoeijmakers JH (1996). "Cloning, comparative mapping, and RNA expression of the mouse homologues of the Saccharomyces cerevisiae nucleotide excision repair gene RAD23". Genomics. 31 (1): 20–27. doi:10.1006/geno.1996.0004. hdl:1765/3097. PMID 8808275.
• Li L, Lu X, Peterson C, Legerski R (1997). "XPC interacts with both HHR23B and HHR23A in vivo". Mutat. Res. 383 (3): 197–203. doi:10.1016/s0921-8777(97)00002-5. PMID 9164480.
• Withers-Ward ES, Jowett JB, Stewart SA, Xie YM, Garfinkel A, Shibagaki Y, Chow SA, Shah N, Hanaoka F, Sawitz DG, Armstrong RW, Souza LM, Chen IS (1997). "Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair". J. Virol. 71 (12): 9732–42. doi:10.1128/JVI.71.12.9732-9742.1997. PMC 230283. PMID 9371639.
• Schauber C, Chen L, Tongaonkar P, Vega I, Lambertson D, Potts W, Madura K (1998). "Rad23 links DNA repair to the ubiquitin/proteasome pathway". Nature. 391 (6668): 715–718. doi:10.1038/35661. PMID 9490418. S2CID 4417020.
• Gragerov A, Kino T, Ilyina-Gragerova G, Chrousos GP, Pavlakis GN (1998). "HHR23A, the human homologue of the yeast repair protein RAD23, interacts specifically with Vpr protein and prevents cell cycle arrest but not the transcriptional effects of Vpr". Virology. 245 (2): 323–330. doi:10.1006/viro.1998.9138. PMID 9636371.
• Dieckmann T, Withers-Ward ES, Jarosinski MA, Liu CF, Chen IS, Feigon J (1998). "Structure of a human DNA repair protein UBA domain that interacts with HIV-1 Vpr". Nat. Struct. Biol. 5 (12): 1042–1047. doi:10.1038/4220. PMID 9846873. S2CID 30478711.
• Kumar S, Talis AL, Howley PM (1999). "Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination". J. Biol. Chem. 274 (26): 18785–18792. doi:10.1074/jbc.274.26.18785. PMID 10373495.
• Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–28025. doi:10.1074/jbc.274.39.28019. PMID 10488153.
• Miao F, Bouziane M, Dammann R, Masutani C, Hanaoka F, Pfeifer G, O'Connor TR (2000). "3-Methyladenine-DNA glycosylase (MPG protein) interacts with human RAD23 proteins". J. Biol. Chem. 275 (37): 28433–28438. doi:10.1074/jbc.M001064200. PMID 10854423.
• Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–1803. doi:10.1093/hmg/9.12.1795. PMID 10915768.
• Withers-Ward ES, Mueller TD, Chen IS, Feigon J (2001). "Biochemical and structural analysis of the interaction between the UBA(2) domain of the DNA repair protein HHR23A and HIV-1 Vpr". Biochemistry. 39 (46): 14103–14112. doi:10.1021/bi0017071. PMID 11087358.
• Zhu Q, Wani G, Wani MA, Wani AA (2001). "Human homologue of yeast Rad23 protein A interacts with p300/cyclic AMP-responsive element binding (CREB)-binding protein to down-regulate transcriptional activity of p53". Cancer Res. 61 (1): 64–70. PMID 11196199.
• Mansky LM, Preveral S, Le Rouzic E, Bernard LC, Selig L, Depienne C, Benarous R, Benichou S (2001). "Interaction of human immunodeficiency virus type 1 Vpr with the HHR23A DNA repair protein does not correlate with multiple biological functions of Vpr". Virology. 282 (1): 176–185. doi:10.1006/viro.2000.0791. PMID 11259200.
• Gaynor EM, Chen IS (2001). "Analysis of apoptosis induced by HIV-1 Vpr and examination of the possible role of the hHR23A protein". Exp. Cell Res. 267 (2): 243–257. doi:10.1006/excr.2001.5247. PMID 11426943.
• Chen L, Shinde U, Ortolan TG, Madura K (2002). "Ubiquitin-associated (UBA) domains in Rad23 bind ubiquitin and promote inhibition of multi-ubiquitin chain assembly". EMBO Rep. 2 (10): 933–938. doi:10.1093/embo-reports/kve203. PMC 1084081. PMID 11571271.
• Elder RT, Song XQ, Chen M, Hopkins KM, Lieberman HB, Zhao Y (2002). "Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination". Nucleic Acids Res. 30 (2): 581–591. doi:10.1093/nar/30.2.581. PMC 99819. PMID 11788722.