VRK1

Serine/threonine-protein kinase VRK1 is an enzyme that in humans is encoded by the VRK1 gene.[5][6]

VRK1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2KTY, 2KUL, 2LAV, 2RSV, 3OP5
Identifiers
Aliases	VRK1, PCH1, PCH1A, vaccinia related kinase 1, VRK serine/threonine kinase 1
External IDs	OMIM: 602168 MGI: 1261847 HomoloGene: 2541 GeneCards: VRK1
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q32.2 Start 96,797,382 bp[1] End 96,931,722 bp[1]
Gene location (Mouse) Chr. Chromosome 12 (mouse)[2] Band 12|12 E-F1 Start 106,010,228 bp[2] End 106,077,426 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • nucleotide binding • protein kinase activity • kinase activity • nucleosomal histone binding • protein serine/threonine kinase activity • GO:0001948 protein binding • histone kinase activity (H3-T3 specific) • histone kinase activity (H3-S10 specific) • ATP binding • protein kinase binding Cellular component • spindle • nucleoplasm • nucleolus • Golgi stack • cytoskeleton • cell nucleus • cytoplasm • cytosol Biological process • phosphorylation • histone H3-S10 phosphorylation • histone H3-T3 phosphorylation • cell division • protein phosphorylation • mitotic nuclear envelope disassembly • cell cycle • mitotic nuclear envelope reassembly • Golgi disassembly • regulation of cell shape • autophosphorylation • peptidyl-serine phosphorylation • peptidyl-threonine phosphorylation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	7443	22367
Ensembl	ENSG00000100749	ENSMUSG00000021115
UniProt	Q99986	Q80X41
RefSeq (mRNA)	NM_003384	NM_001029843 NM_001029844 NM_011705 NM_001364365 NM_001364367 NM_001364368
RefSeq (protein)	NP_003375	NP_001025014 NP_001025015 NP_035835 NP_001351294 NP_001351296 NP_001351297
Location (UCSC)	Chr 14: 96.8 – 96.93 Mb	Chr 12: 106.01 – 106.08 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Cartoon diagram of the Human vaccinia-related kinase protein PDB entry 6bru

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and C-jun.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000100749 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000021115 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nezu J, Oku A, Jones MH, Shimane M (Feb 1998). "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase". Genomics. 45 (2): 327–31. doi:10.1006/geno.1997.4938. PMID 9344656.
6. "Entrez Gene: VRK1 vaccinia related kinase 1".

Further reading

• Sugimoto J, Yamauchi T, Hatakeyama T, Isobe M (1999). "Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus". J. Hum. Genet. 44 (2): 133–4. doi:10.1007/s100380050127. PMID 10083742.
• Sayed M, Kim SO, Salh BS, et al. (2000). "Stress-induced activation of protein kinase CK2 by direct interaction with p38 mitogen-activated protein kinase". J. Biol. Chem. 275 (22): 16569–73. doi:10.1074/jbc.M000312200. PMID 10747897.
• Lopez-Borges S, Lazo PA (2000). "The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein". Oncogene. 19 (32): 3656–64. doi:10.1038/sj.onc.1203709. PMID 10951572.
• Latonen L, Taya Y, Laiho M (2001). "UV-radiation induces dose-dependent regulation of p53 response and modulates p53-HDM2 interaction in human fibroblasts". Oncogene. 20 (46): 6784–93. doi:10.1038/sj.onc.1204883. PMID 11709713.
• Barcia R, López-Borges S, Vega FM, Lazo PA (2002). "Kinetic properties of p53 phosphorylation by the human vaccinia-related kinase 1". Arch. Biochem. Biophys. 399 (1): 1–5. doi:10.1006/abbi.2001.2746. PMID 11883897.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Nichols RJ, Traktman P (2004). "Characterization of three paralogous members of the Mammalian vaccinia related kinase family". J. Biol. Chem. 279 (9): 7934–46. doi:10.1074/jbc.M310813200. PMID 14645249.
• Boyle KA, Traktman P (2004). "Members of a novel family of mammalian protein kinases complement the DNA-negative phenotype of a vaccinia virus ts mutant defective in the B1 kinase". J. Virol. 78 (4): 1992–2005. doi:10.1128/JVI.78.4.1992-2005.2004. PMC 369515. PMID 14747564.
• Sevilla A, Santos CR, Vega FM, Lazo PA (2004). "Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK". J. Biol. Chem. 279 (26): 27458–65. doi:10.1074/jbc.M401009200. PMID 15105425.
• Sevilla A, Santos CR, Barcia R, et al. (2004). "c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK)" (PDF). Oncogene. 23 (55): 8950–8. doi:10.1038/sj.onc.1208015. PMID 15378002.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Vega FM, Sevilla A, Lazo PA (2005). "p53 Stabilization and accumulation induced by human vaccinia-related kinase 1". Mol. Cell. Biol. 24 (23): 10366–80. doi:10.1128/MCB.24.23.10366-10380.2004. PMC 529057. PMID 15542844.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
• Santos CR, Rodríguez-Pinilla M, Vega FM, et al. (2006). "VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma". Mol. Cancer Res. 4 (3): 177–85. doi:10.1158/1541-7786.MCR-05-0212. PMID 16547155.
• Valbuena A, Vega FM, Blanco S, Lazo PA (2006). "p53 downregulates its activating vaccinia-related kinase 1, forming a new autoregulatory loop". Mol. Cell. Biol. 26 (13): 4782–93. doi:10.1128/MCB.00069-06. PMC 1489172. PMID 16782868.
• Valbuena A, López-Sánchez I, Vega FM, et al. (2007). "Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations". Arch. Biochem. Biophys. 465 (1): 219–26. doi:10.1016/j.abb.2007.06.005. PMID 17617371.