Calsequestrin

Calsequestrin is a calcium-binding protein that acts as a calcium buffer within the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. It also helps the sarcoplasmic reticulum store an extraordinarily high amount of calcium ions. Each molecule of calsequestrin can bind 18 to 50 Ca2+ ions.[1] Sequence analysis has suggested that calcium is not bound in distinct pockets via EF-hand motifs, but rather via presentation of a charged protein surface. Two forms of calsequestrin have been identified. The cardiac form Calsequestrin-2 (CASQ2) is present in cardiac and slow skeletal muscle and the fast skeletal form Calsequestrin-1(CASQ1) is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation.[2] When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%.[2] Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree.[3] Calsequestrin is also secreted in the gut where it deprives bacteria of calcium ions..

calsequestrin 1 (fast-twitch, skeletal muscle)
Calsequestrin monomer showing the three repeating calsequestrin domains
Identifiers
SymbolCASQ1
Alt. symbolsCASQ
NCBI gene844
HGNC1512
OMIM114250
PDB1A8Y
RefSeqNM_001231
UniProtP31415
Other data
LocusChr. 1 q21
calsequestrin 2 (cardiac muscle)
Identifiers
SymbolCASQ2
NCBI gene845
HGNC1513
OMIM114251
RefSeqNM_001232
UniProtO14958
Other data
LocusChr. 1 p13.3-p11
Calsequestrin
crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum at 2.4 a resolution
Identifiers
SymbolCalsequestrin
PfamPF01216
Pfam clanCL0172
InterProIPR001393
PROSITEPDOC00675
SCOP21a8y / SCOPe / SUPFAM

Cardiac calsequestrin

Cardiac calsequestrin (CASQ2) plays an integral role in cardiac regulation. Mutations in the cardiac calsequestrin gene have been associated with cardiac arrhythmia and sudden death.[4] CASQ2 is thought to have a role in regulating cardiac excitation-contraction coupling and calcium-induced calcium release (CICR) in the heart, as overexpression of CASQ2 has been shown to substantially raise the magnitude of cell-averaged ICA-induced calcium transients and spontaneous calcium sparks in isolated heart cells.[4] Furthermore, CASQ2 modulates the CICR mechanism by lengthening to process to functionally recharge the sarcoplamsic reticulum's calcium ion stores.[4] A lack of or mutation in CSQ2 has been directly associated with catecholaminergic polymorphic ventricular tachycardia (CPVT).[5] A mutation can have a significant effect if it disrupts the linear polymerization ability of CASQ2, which directly accounts for its high-capacity to bind Ca2+.[5] In addition, the hydrophobic core of domain II appears to be necessary for CASQ2's function, because a single amino acid mutation that disrupts this hydrophobic core directly leads to molecular aggregates, which are unable to respond to calcium ions.[5]

See also

References
  1. Katz, Arnold M. (2005). Physiology of the Heart (4th ed.). Lippincott Williams & Wilkins. p. 192. ISBN 978-0-7817-5501-6.
  2. Slupsky JR, Ohnishi M, Carpenter MR, Reithmeier RA (October 1987). "Characterization of cardiac calsequestrin". Biochemistry. 26 (20): 6539–44. doi:10.1021/bi00394a038. PMID 3427023.
  3. Cala SE, Jones LR (January 1991). "Phosphorylation of cardiac and skeletal muscle calsequestrin isoforms by casein kinase II. Demonstration of a cluster of unique rapidly phosphorylated sites in cardiac calsequestrin". J. Biol. Chem. 266 (1): 391–8. doi:10.1016/S0021-9258(18)52447-9. PMID 1985907.
  4. Gryoke, Sandor (2003). "Calsequestrin determines the functional size and stability of cardiac intracellular calcium stores: Mechanism for hereditary arrhythmia". Proceedings of the National Academy of Sciences of the United States of America. 100 (20): 11759–11764. Bibcode:2003PNAS..10011759T. doi:10.1073/pnas.1932318100. PMC 208831. PMID 13130076.
  5. Kim, EunJung; Youn, Buhyun; Kemper, Lenord; Campbell, Cait; Milting, Hendrik; Varsanyi, Magdolna; Kang, ChulHee (2007-11-02). "Characterization of Human Cardiac Calsequestrin and its Deleterious Mutants". Journal of Molecular Biology. 373 (4): 1047–1057. doi:10.1016/j.jmb.2007.08.055. PMID 17881003.

Further reading
  • Wang S, Trumble WR, Liao H, Wesson CR, Dunker AK, Kang CH (1998). "Crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum". Nat. Struct. Biol. 5 (6): 476–83. doi:10.1038/nsb0698-476. PMID 9628486. S2CID 7967757.
This article incorporates text from the public domain Pfam and InterPro: IPR001393


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.