EIF4B

Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene.[5]

EIF4B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1WI8, 2J76
Identifiers
Aliases	EIF4B, EIF-4B, PRO1843, eukaryotic translation initiation factor 4B
External IDs	OMIM: 603928 MGI: 95304 HomoloGene: 83162 GeneCards: EIF4B
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q13.13 Start 53,006,158 bp[1] End 53,042,209 bp[1]
Gene location (Mouse) Chr. Chromosome 15 (mouse)[2] Band 15|15 F2 Start 102,073,773 bp[2] End 102,097,173 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • nucleic acid binding • ribosomal small subunit binding • GO:0008026 helicase activity • RNA strand-exchange activity • RNA strand annealing activity • RNA binding • translation initiation factor activity Cellular component • cytosol • eukaryotic translation initiation factor 4F complex • polysome Biological process • nuclear-transcribed mRNA poly(A) tail shortening • regulation of translational initiation • formation of translation preinitiation complex • eukaryotic translation initiation factor 4F complex assembly • protein biosynthesis • translational initiation • cytoplasmic translation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1975	75705
Ensembl	ENSG00000063046	ENSMUSG00000058655
UniProt	P23588	Q8BGD9
RefSeq (mRNA)	NM_001300821 NM_001417 NM_018507 NM_001330654	NM_145625
RefSeq (protein)	NP_001287750 NP_001317583 NP_001408	NP_663600
Location (UCSC)	Chr 12: 53.01 – 53.04 Mb	Chr 15: 102.07 – 102.1 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

eIF4B has been shown to interact with and stimulate the activity of eIF4A and bind to the eIF3 complex through the eIF3A subunit.[6] This interaction results in the recruitment of the eukaryotic small ribosomal subunit (40S) to the mRNA which will in turn set the stage for the later steps leading to elongation.

See also

• Eukaryotic translation
• eIF4F

References

1. GRCh38: Ensembl release 89: ENSG00000063046 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000058655 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: EIF4B eukaryotic translation initiation factor 4B".
6. Merrick WC (Oct 2015). "eIF4F: a retrospective". The Journal of Biological Chemistry. 290 (40): 24091–9. doi:10.1074/jbc.R115.675280. PMC 4591800. PMID 26324716.

Further reading

• van Heugten HA, Kasperaitis MA, Thomas AA, Voorma HO (1991). "Evidence that eukaryotic initiation factor (eIF) 2 is a cap-binding protein that stimulates cap recognition by eIF-4B and eIF-4F". J. Biol. Chem. 266 (11): 7279–84. PMID 2016328.
• Milburn SC, Hershey JW, Davies MV, Kelleher K, Kaufman RJ (1990). "Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif". EMBO J. 9 (9): 2783–90. doi:10.1002/j.1460-2075.1990.tb07466.x. PMC 551988. PMID 2390971.
• Howe JG, Hershey JW (1984). "Translational initiation factor and ribosome association with the cytoskeletal framework fraction from HeLa cells". Cell. 37 (1): 85–93. doi:10.1016/0092-8674(84)90303-9. PMID 6722878.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Méthot N, Pause A, Hershey JW, Sonenberg N (1994). "The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence". Mol. Cell. Biol. 14 (4): 2307–16. doi:10.1128/mcb.14.4.2307. PMC 358597. PMID 8139536.
• Naranda T, Strong WB, Menaya J, Fabbri BJ, Hershey JW (1994). "Two structural domains of initiation factor eIF-4B are involved in binding to RNA". J. Biol. Chem. 269 (20): 14465–72. PMID 8182051.
• Méthot N, Song MS, Sonenberg N (1996). "A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3". Mol. Cell. Biol. 16 (10): 5328–34. doi:10.1128/MCB.16.10.5328. PMC 231531. PMID 8816444.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Richter NJ, Rogers GW, Hensold JO, Merrick WC (1999). "Further biochemical and kinetic characterization of human eukaryotic initiation factor 4H". J. Biol. Chem. 274 (50): 35415–24. doi:10.1074/jbc.274.50.35415. PMID 10585411.
• Bushell M, Wood W, Clemens MJ, Morley SJ (2000). "Changes in integrity and association of eukaryotic protein synthesis initiation factors during apoptosis". Eur. J. Biochem. 267 (4): 1083–91. doi:10.1046/j.1432-1327.2000.01101.x. PMID 10672017.
• Bushell M, Wood W, Carpenter G, Pain VM, Morley SJ, Clemens MJ (2001). "Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages". J. Biol. Chem. 276 (26): 23922–8. doi:10.1074/jbc.M100384200. PMID 11274152.
• Fleming K, Ghuman J, Yuan X, Simpson P, Szendröi A, Matthews S, Curry S (2003). "Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B". Biochemistry. 42 (30): 8966–75. doi:10.1021/bi034506g. PMID 12885229.
• Raught B, Peiretti F, Gingras AC, Livingstone M, Shahbazian D, Mayeur GL, Polakiewicz RD, Sonenberg N, Hershey JW (2004). "Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases". EMBO J. 23 (8): 1761–9. doi:10.1038/sj.emboj.7600193. PMC 394247. PMID 15071500.
• Doepker RC, Hsu WL, Saffran HA, Smiley JR (2004). "Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H". J. Virol. 78 (9): 4684–99. doi:10.1128/JVI.78.9.4684-4699.2004. PMC 387725. PMID 15078951.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Shahbazian D, Roux PP, Mieulet V, Cohen MS, Raught B, Taunton J, Hershey JW, Blenis J, Pende M, Sonenberg N (2006). "The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity". EMBO J. 25 (12): 2781–91. doi:10.1038/sj.emboj.7601166. PMC 1500846. PMID 16763566.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.