Myxobolus cerebralis

Myxobolus cerebralis is a myxosporean parasite of salmonids (salmon, trout, and their allies) that causes whirling disease in farmed salmon and trout and also in wild fish populations. It was first described in rainbow trout in Germany a century ago, but its range has spread and it has appeared in most of Europe (including Russia), the United States, South Africa,[1] Canada[2] and other countries. In the 1980s, M. cerebralis was found to require a tubificid oligochaete (a kind of segmented worm) to complete its life cycle.[3] The parasite infects its hosts with its cells after piercing them with polar filaments ejected from nematocyst-like capsules.

Myxobolus cerebralis
Triactinomyxon stage of Myxobolus cerebralis - note the three "tails"
Scientific classification
Kingdom: Animalia
Phylum: Cnidaria
Class: Myxosporea
Order: Bivalvulida
Family: Myxobolidae
Genus: Myxobolus
Species:
M. cerebralis
Binomial name
Myxobolus cerebralis
Hofer, 1903
Synonyms

Myxosoma cerebralis
Triactinomyxon dubium
Triactinomyxon gyrosalmo

Whirling disease afflicts juvenile fish (fingerlings and fry) and causes skeletal deformation and neurological damage. Fish "whirl" forward in an awkward, corkscrew-like pattern instead of swimming normally, find feeding difficult, and are more vulnerable to predators. The mortality rate is high for fingerlings, up to 90% of infected populations, and those that do survive are deformed by the parasites residing in their cartilage and bone. They act as a reservoir for the parasite, which is released into water following the fish's death. M. cerebralis is one of the most economically important myxozoans in fish, as well as one of the most pathogenic. It was the first myxosporean whose pathology and symptoms were described scientifically.[4] The parasite is not transmissible to humans.[5][6][7][8][9][10]

Taxonomy

The taxonomy and naming of both M. cerebralis, and of myxozoans in general, have complicated histories. It was originally thought to infect fish brains (hence the specific epithet cerebralis) and nervous systems, though it soon was found to primarily infect cartilage and skeletal tissue. Attempts to change the name to Myxobolus chondrophagus, which would more accurately describe the organism, failed because of nomenclature rules.[1] Later, the organisms previously called Triactinomyxon dubium and T. gyrosalmo (class Actinosporea) were found to be, in fact, triactinomyxon stages of M. cerebralis, the life cycle of which was expanded to include the triactinomyxon stage.[11] Similarly, other actinosporeans were folded into the life cycles of various myxosporeans.

Today, the myxozoans, previously thought to be multicellular protozoans, are considered animals by most scientists, though their status has not officially changed. Recent molecular studies suggest they are related to Bilateria or Cnidaria, with Cnidaria being closer morphologically because both groups have extrusive filaments.[12] Bilateria were somewhat closer in some genetic studies,[13] but those were found to have used samples that were contaminated by material from the host organism, and a 2015 study confirms they are cnidarians.[14]

Morphology
Diagram of the structure of a triactinomyxon stage spore of Myxobolus cerebralis

M. cerebralis has many diverse stages ranging from single cells to relatively large spores, not all of which have been studied in detail.

Triactinomyxon stage

The stages that infect fish, called triactinomyxon spores, are made of a single style that is about 150 micrometers (μm) long and three processes or "tails", each about 200 micrometers long. A sporoplasm packet at the end of the style contains 64 germ cells surrounded by a cellular envelope.[12] There are also three polar capsules, each of which contains a coiled polar filament between 170 and 180 μm long.[3] Polar filaments in both this stage and in the myxospore stage (see picture above) rapidly shoot into the body of the host, creating an opening through which the sporoplasm can enter.

Sporoplasm stage

Upon contact with fish hosts and firing of the polar capsules, the sporoplasm contained within the central style of the triactinomyxon migrates into the epithelium or gut lining. Firstly, this sporoplasm undergoes mitosis to produce more amoeboid cells, which migrate into deeper tissue layers, to reach the cerebral cartilage.[3]

Myxosporean stage

Myxospores, which develop from sporogonic cell stages inside fish hosts, are lenticular. They have a diameter of about 10 micrometers and are made of six cells. Two of these cells form polar capsules, two merge to form a binucleate sporoplasm, and two form protective valves.[12] Myxospores are infective to oligochaetes, and are found among the remains of digested fish cartilage. They are often difficult to distinguish from related species because of morphological similarities across genera. Though M. cerebralis is the only myxosporean ever found in salmonid cartilage, other visually similar species may be present in the skin, nervous system, or muscle.[3]

Life cycle
Life cycle of M. cerebralis

Myxobolus cerebralis has a two-host life cycle involving a salmonid fish and a tubificid oligochaete. So far, the only worm known to be susceptible to M. cerebralis infection is Tubifex tubifex,[3] though what scientists currently call T. tubifex may in fact be more than one species.[4] First, myxospores are ingested by tubificid worms. In the gut lumen of the worm, the spores extrude their polar capsules and attach to the gut epithelium by polar filaments. The shell valves then open along the suture line and the binucleate germ cell penetrates between the intestinal epithelial cells of the worm. This cell multiplies, producing many amoeboid cells by an asexual cell fission process called merogony. As a result of the multiplication process, the intercellular space of the epithelial cells in more than 10 neighbouring worm segments may become infected.[15]

Around 60–90 days postinfection, sexual cell stages of the parasite undergo sporogenesis, and develop into pansporocysts, each of which contains eight triactinomyxon-stage spores. These spores are released from the oligochaete anus into the water.[12] Alternatively, a fish can become infected by eating an infected oligochaete.[12] Infected tubificids can release triactinomyxons for at least a year.[15] The triactinomyxon spores are carried by the water currents, where they can infect a salmonid through the skin. Penetration of the fish by these spores takes only a few seconds. Within five minutes, a sac of germ cells called a sporoplasm has entered the fish epidermis, and within a few hours, the sporoplasm splits into individual cells that will spread through the fish.[12]

Within the fish, both intracellular and extracellular stages reproduce in its cartilage by asexual endogeny, meaning new cells grow from within old cells. The final stage within the fish is the creation of the myxospore, which is formed by sporogony. They are released into the environment when the fish decomposes or is eaten.[12] Some recent research indicates some fish may expel viable myxospores while still alive.[16]

Myxospores are extremely tough: "it was shown that Myxobolus cerebralis spores can tolerate freezing at −20°C for at least 3 months, aging in mud at 13°C for at least 5 months, and passage through the guts of northern pike Esox lucius or mallards Anas platyrhynchos without loss of infectivity" to worms.[17] Triactinomyxons are much shorter-lived, surviving 34 days or less, depending on temperature.[18]

Pathology
Skeletal deformation in a mature brook trout caused by M. cerebralis infection.

M. cerebralis infections have been reported from a wide range of salmonid species: eight species of "Atlantic" salmonids, Salmo; four species of "Pacific" salmonids, Oncorhynchus; four species of char, Salvelinus; the grayling, Thymallus thymallus; and the huchen, Hucho hucho.[19] M. cerebralis causes damage to its fish hosts through attachment of triactinomyxon spores and the migrations of various stages through tissues and along nerves, as well as by digesting cartilage.[12] The fish's tail may darken, but aside from lesions on cartilage, internal organs generally appear healthy.[3] Other symptoms include skeletal deformities and "whirling" behavior (tail-chasing) in young fish, which was thought to have been caused by a loss of equilibrium, but is actually caused by damage to the spinal cord and lower brain stem.[4] Experiments have shown that fish can kill Myxobolus in their skin (possibly using antibodies), but that the fish do not attack the parasites once they have migrated to the central nervous system. This response varies from species to species.[4]

In T. tubifex, the release of triactinomyxon spores from the intestinal wall damages the worm's mucosa; this may happen thousands of times in a single worm, and is believed to impair nutrient absorption.[12] Spores are released from the worm almost exclusively when the temperature is between 10 °C and 15 °C, so fish in warmer or cooler waters are less likely to be infected, and infection rates vary seasonally.[4]

Susceptibility
See also: Salmonid susceptibility to whirling disease

Fish size, age, concentration of triactinomyxon spores, and water temperature all affect infection rates in fish, as does the species of the fish in question.[20] The disease has the most impact on fish less than five months old because their skeletons have not ossified. This makes young fish more susceptible to deformities[21] and provides M. cerebralis more cartilage on which to feed.[4] In one study of seven species of many strains, brook trout and rainbow trout (except one strain) were far more heavily affected by M. cerebralis after two hours of exposure than other species were, while bull trout, Chinook salmon, brown trout, and Arctic grayling were least severely affected.[20] While brown trout may harbor the parasite, they typically do not show any symptoms, and this species may have been M. cerebralis' original host.[22] This lack of symptoms in brown trout meant that the parasite was only discovered after nonnative rainbow trouts were introduced in Europe.[4]

Diagnosis
The normally uniform trout cartilage is scarred with lesions in which M. cerebralis spores develop, weakening and deforming the connective tissues.

Moderate or heavy clinical infection of fish with whirling disease can be presumptively diagnosed on the basis of changes in behavior and appearance about 35 to 80 days after initial infection, though "injury or deficiency in dietary tryptophan and ascorbic acid can evoke similar signs", so conclusive diagnosis may require finding myxospores in the fish's cartilage.[3] In heavy infections, only examining cartilage microscopically may be necessary to find spores.[3] In less severe infections, the most common test involves digestion of the cranial cartilage with the proteases pepsin and trypsin (pepsin-trypsin digest—PTD) before looking for spores. The head and other tissues can be further examined using histopathology to confirm whether the location and morphology of the spores matches what is known for M. cerebralis. Serological identification of spores in tissue sections using an antibody raised against the spores is also possible. Parasite identity can also be confirmed using polymerase chain reaction to amplify the 415 base pair 18S rRNA gene from M. cerebralis.[23] Fish should be screened at the life stage most susceptible to the parasites, with particular focus on fish in aquaculture units.[24]

Impact
M. cerebralis has been reported in Germany (1893), Italy (1954), USSR (1955), including Sakhalin Island (1960), USA (1958), Bulgaria (1960), Yugoslavia (1960), Sweden (1966), South Africa (1966), Scotland (1968), New Zealand (1971), Ecuador (1971), Norway (1971), Colombia (1972), Lebanon (1973), Ireland (1974), Spain (1981) and England (1981)

Although originally a mild pathogen of Salmo trutta in central Europe and other salmonids in northeast Asia, the introduction of the rainbow trout (Oncorhynchus mykiss) has greatly increased the impact of this parasite. Having no innate immunity to M. cerebralis, rainbow trout are particularly susceptible, and can release so many spores that even more resistant species in the same area, such as S. trutta, can become overloaded with parasites and incur 80%–90% mortalities. Where M. cerebralis has become well-established, it has caused decline or even elimination of whole cohorts of fish.[25][26]

Impact in Europe

The impact of M. cerebralis in Europe is somewhat lessened because the species is endemic to this region, giving native fish stocks a degree of immunity. Rainbow trout, the most susceptible species to this parasite, are not native to Europe; successfully reproducing feral populations are rare, so few wild rainbow trout are young enough to be susceptible to infection. On the other hand, they are widely reared for restocking sport-fishing waters and for aquaculture, where this parasite has its greatest impact. Hatching and rearing methods designed to prevent infection of rainbow trout fry have proved successful in Europe. These techniques include hatching eggs in spore-free water and rearing fry to the "ossification" stage in tanks or raceways. These methods give particular attention to the quality of water sources to guard against spore introduction during water exchanges.[27] Fry are moved to earthen ponds only once they are considered to be clinically resistant to the parasite, after skeletal ossification occurs.[21]

Impact in New Zealand

M. cerebralis was first found in New Zealand in 1971. The parasite has only been found in rivers in the South Island, away from the most important aquaculture sites. Additionally, salmonid species commercially aquacultured in New Zealand have low susceptibility to whirling disease, and the parasite has also not been shown to affect native salmonids.[28] An important indirect effect of the parasites presence is quarantine restriction placed on exports of salmon products to Australia.[28]

Impact in the United States
M. cerebralis has been reported in nearly two dozen (green) states in the United States, according to the Whirling Disease Initiative

M. cerebralis was first recorded in North America in 1956 in Pennsylvania, having been introduced via infected trout imported from Europe, and has spread steadily south and westwards.[29] Until the 1990s, whirling disease was considered a manageable problem affecting rainbow trout in hatcheries. However, it has recently become established in natural waters of the Rocky Mountain states (Colorado, Wyoming, Utah, Montana, Idaho, New Mexico), where it is causing heavy mortalities in several sportfishing rivers. Some streams in the western United States have lost 90% of their trout.[30] In addition, whirling disease threatens recreational fishing, which is important for the tourism industry, a key component of the economies of some U.S. western states. For example, "the Montana Whirling Disease Task Force estimated trout fishing generated US $300,000,000 in recreational expenditures in Montana alone".[4] Making matters worse, some of the fish species that M. cerebralis infects (bull trout, cutthroat trout, and steelhead) are already threatened or endangered, and the parasite could worsen their already precarious situations.[4] For reasons that are poorly understood, but probably have to do with environmental conditions, the impact on infected fish has been greatest in Colorado and Montana, and least in California, Michigan, and New York.[31]

Impact in Canada

Whirling disease was first detected in fish in Johnson Lake in Banff National Park in May, 2016. CFIA Labs confirmed in August and Parks Canada announced the outbreak August 23, 2016.[32] Although it was first discovered in Banff, it is not necessarily where the disease originated and spread. The Government of Alberta is currently sampling and testing fish in 6 different watersheds (Peace River, Athabasca, North Saskatchewan, Red Deer, Bow and Oldman) to see where the disease has spread. Initial sample fish were collected in 2016, and are currently being processed by the Government of Alberta and CFIA labs. Since testing began, it has been detected in the Upper Bow River,[33] and in May 2017 it was confirmed that whirling disease had also been detected in the Oldman River Basin.[34] The declaration does not mean that every susceptible finfish population within the Bow and Oldman River watersheds are infected with the disease.

As a result of the new declaration, a domestic movement permit will be required from the CFIA for susceptible species and end uses identified in the Domestic Movement Control Program, the vector Tubifex tubifex, the disease causing agent Myxobolus cerebralis, and/or related things out of the infected and buffer areas of Alberta. Recreational and sport fishing, including fishing led by a professional guide, will not require a CFIA permit.[35]

Prevention and control

Some biologists have attempted to disarm triactinomyxon spores by making them fire prematurely. In the laboratory, only extreme acidity or basicity, moderate to high concentrations of salts, or electric current caused premature filament discharge; neurochemicals, cnidarian chemosensitizers, and trout mucus were ineffective,[36] as were anesthetized or dead fish.[37] If spores could be disarmed, they would be unable to infect fish, but further research is needed to find an effective treatment.[36]

Some strains of fish are more resistant than others, even within species;[20] using resistant strains may help reduce the incidence and severity of whirling disease in aquaculture. There is also some circumstantial evidence that fish populations can develop resistance to the disease over time.[38] Additionally, aquaculturists may avoid M. cerebralis infections by not using earthen ponds for raising young fish; this keeps them away from possibly infected tubificids and makes it easier to eliminate spores and oligochaetes through filtration, chlorination, and ultraviolet bombardment.[3] To minimise tubificid populations, techniques include periodic disinfection of the hatchery or aquaculture ponds, and the rearing of small trout indoors in pathogen-free water. Smooth-faced concrete or plastic-lined raceways that are kept clean and free of contaminated water keep aquaculture facilities free of the disease.[3]

Lastly, some drugs, such as furazolidone, furoxone, benomyl, fumagillin, proguanil and clamoxyquine, have been shown to impede spore development, which reduces infection rates.[3] For example, one study showed that feeding fumagillin to O. mykiss reduced the number of infected fish from between 73% and 100% to between 10% and 20%.[17] Unfortunately, this treatment is considered unsuitable for wild trout populations,[15] and no drug treatment has ever been shown to be effective in the studies required for United States Food and Drug Administration approval.[4]

Recreational and sports fishers can help to prevent the spread of the parasite by not transporting fish from one body of water to another, not disposing of fish bones or entrails in any body of water, and ensuring boots and shoes are clean before moving between different bodies of water. Federal, state, provincial, and local regulations on the use of bait should be followed.[39]

See also

Notes
  1. Bartholomew, J.L.; Reno, P.W. (2002). "The history and dissemination of whirling disease". American Fisheries Society Symposium. 29: 3–24.
  2. "All the fish in this Banff lake are to be removed and killed to protect other lakes from whirling disease".
  3. Markiw, M.E. (1992). "Salmonid Whirling disease". Fish and Wildlife Leaflet. 17: 1–3. Archived from the original on 2004-07-10.
  4. Gilbert, M. A.; Granath, W. O. Jr. (2003). "Whirling disease and salmonid fish: life cycle, biology, and disease". Journal of Parasitology. 89 (4): 658–667. doi:10.1645/ge-82r. JSTOR 3285855. PMID 14533670. S2CID 8950955.
  5. "Whirling Disease - Yellowstone National Park (U.S. National Park Service)".
  6. "Whirling Disease - Stop Aquatic Hitchhikers".
  7. "Whirling disease - Utah Division of Wildlife Resources".
  8. "Colorado Parks & Wildlife - Whirling Disease and Colorado's Trout".
  9. "What is Whirling Disease? - North Central Regional Aquaculture Center - Mohamed Faisal - Donald Garling" (PDF).
  10. "Whirling disease | Alberta.ca".
  11. Kent, M. L.; Margolis, L.; Corliss, J.O. (1994). "The demise of a class of protists: taxonomic and nomenclatural revisions proposed for the protist phylum Myxozoa Grasse, 1970". Canadian Journal of Zoology. 72 (5): 932–937. doi:10.1139/z94-126.
  12. Hedrick, R. P.; El-Matbouli, M. (2002). "Recent advances with taxonomy, life cycle, and development of Myxobolus cerebralis in the fish and oligochaete hosts". American Fisheries Society Symposium. 29: 45–53.
  13. Monteiro, A. S.; Okamura, B.; Holland, P. W. H. (2002). "Orphan worm finds a home: Buddenbrockia is a Myxozoan". Molecular Biology and Evolution. 19 (6): 968–971. doi:10.1093/oxfordjournals.molbev.a004155. PMID 12032254. Archived from the original on 2005-04-11.
  14. Chang, E. Sally; Neuhof, Moran; Rubinstein, Nimrod D.; Diamant, Arik; Philippe, Hervé; Huchon, Dorothée; Cartwright, Paulyn (2015-12-01). "Genomic insights into the evolutionary origin of Myxozoa within Cnidaria". Proceedings of the National Academy of Sciences. 112 (48): 14912–14917. Bibcode:2015PNAS..11214912C. doi:10.1073/pnas.1511468112. ISSN 0027-8424. PMC 4672818. PMID 26627241.
  15. El-Matbouli, M.; Hoffmann, R.W. (1998). "Light and electron microscopic studies on the chronological development of Myxobolus cerebralis to the Actinosporean stage in Tubifex Tubifes". International Journal for Parasitology. 28 (1): 195–217. doi:10.1016/s0020-7519(97)00176-8. PMID 9504346.
  16. Nehring, K. A.; Thompson, R. B.; Taurman, K. G.; Shuler, D.L. (2002). "Laboratory studies indicating that living brown trout Salmo trutta expel viable Myxobolus cerebralis myxospores". American Fisheries Society Symposium. 29: 125–134.
  17. El-Matbouli, M.; Hoffmann, R.W. (1991). "Effects of freezing, aging, and passage through the alimentary canal of predatory animals on the viability of Myxobolus cerebralis spores". Journal of Aquatic Animal Health. 3 (4): 260–262. doi:10.1577/1548-8667(1991)003<0260:eofaap>2.3.co;2.
  18. Markiw, M.E. (1992). "Experimentally induced whirling disease. II. Determination of longevity of the infective triactinomyxon stage of Myxobolus cerebralis by vital staining". Journal of Aquatic Animal Health. 4 (1): 44–47. doi:10.1577/1548-8667(1992)004<0044:eiwdid>2.3.co;2.
  19. Lom, J. & Dyková, I. (1992). Protozoan Parasites of Fishes, Elsevier, Amsterdam. ISBN 0-444-89434-9.
  20. Vincent, E. R. (2002). "Relative susceptibility of various salmonids to whirling disease with emphasis on rainbow and cutthroat trout". American Fisheries Society Symposium. 29: 109–115.
  21. Halliday, M.M. (1976). "The Biology of Myxosoma cerebralis: The Causative Organism of Whirling Disease of Salmonids". Journal of Fish Biology. 9 (4): 339–357. doi:10.1111/j.1095-8649.1976.tb04683.x.
  22. Hoffmann, G (1962). "Whirling Disease Of Trout". U.S. Department of the Interior, Fishery Leaflet. 508: 1–3.
  23. Andree, K.B.; MacConnell, E.; Hedrick, R.P. (1998). "A nested polymerase chain reaction for the detection of genomic DNA of Myxobolus cerebralis in rainbow trout Oncorhynchus mykiss". Diseases of Aquatic Organisms. 34 (2): 145–54. doi:10.3354/dao034145. PMID 9828408.
  24. "5.2 Myxobolus cerebralis (Whirling Disease)" (PDF). American Fisheries Society Bluebook. Fish and Wildlife Service. 2004.
  25. Nehring, R.B. (1996). "Whirling Disease In Feral Trout Populations In Colorado." In E.P. Bergersen And B.A.Knoph (eds.), Proceedings: Whirling Disease Workshop––where Do We Go From Here? Colorado Cooperative Fish And Wildlife Research Unit, Fort Collins.: pp.159.
  26. Vincent, E.R. (1996). "Whirling Disease—the Montana Experience, Madison River." In, E.P. Bergersen And B.A.Knoph (eds.), Proceedings: Whirling Disease Workshop—where Do We Go From Here? Colorado Cooperative Fish And Wildlife Research Unit, Fort Collins.: pp.159.
  27. Ghittino, P (1970). "Present Status of Whirling Disease In Italian Trout Farms". Riv. It. Piscic. Ittiopat. 5: 89–92.
  28. Stone M A B, MacDiarmid S C, Pharo H J. (1997). Import health risk analysis: salmonids for human consumption. Ministry of Agriculture Regulatory Authority, New Zealand.
  29. Bergersen, E.P.; Anderson, D.E. (1997). "The distribution and spread of Myxobolus cerebralis in the United States". Fisheries. 22 (8): 6–7. doi:10.1577/1548-8446(1997)022<0006:tdasom>2.0.co;2.
  30. Tennyson, J. Anacker, T. & Higgins, S. (January 13, 1997). "Scientific breakthrough helps combat trout disease." U.S. Fish and Wildlife Service Whirling Disease Foundation News Release."Archived copy". Archived from the original on 2005-06-16. Retrieved 2006-01-03.CS1 maint: archived copy as title (link)
  31. Wisconsin Department of Agriculture, Trade and Consumer Protection. Division of Animal Health. (October 2001). "Fish Health Advisory: Whirling Disease in Trout." "Archived copy" (PDF). Archived from the original (PDF) on 2004-06-26. Retrieved 2005-05-17.CS1 maint: archived copy as title (link) (.pdf).
  32. Parks Canada. (2016). 'Whirling disease confirmed'
  33. CBC News. (2016). 'Whirling disease confirmed in Bow River, CFIA says'
  34. CBC News. (2017). 'Whirling disease now infects entire Oldman River basin, including Waterton Lakes National Park'
  35. Directorate, Government of Canada, Canadian Food Inspection Agency, Animal Health. "Notice to Industry – Update on Zoning of Alberta for Whirling Disease". www.inspection.gc.ca. Retrieved 2017-05-17.
  36. Wagner, E. J.; Cannon, Q.; Smith, M.; Hillyard, R.; Arndt, R. (2002). "Extrusion of Polar Filaments of the Myxobolus cerebralis Triactinomyxon by salts, electricity, and other agents" (PDF). American Fisheries Society Symposium. 29: 61–76.
  37. El-Matbouli, M., Hoffmann, R.W., Shoel, H., McDowell, T. S., & Hedrick, R.P. (1999)." Whirling disease: host specificity and interaction between the actinosporean stage of Myxobolus cerebralis and rainbow trout (Oncorhynchus mykiss) cartilage." Diseases of Aquatic Organisms 35: 1–12.
  38. Whirling Disease Foundation News. July, 2003. Research on whirling disease resistant rainbow trout Archived 2007-07-31 at the Wayback Machine
  39. Myxobolus cerebralis. (16 August 2012). USGS Nonindigenous Aquatic Species Database, Gainesville, FL, and NOAA Great Lakes Aquatic Nonindigenous Species Information System, Ann Arbor, MI.


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