TGF alpha

Transforming growth factor alpha (TGF-α) is a protein that in humans is encoded by the TGFA gene.[5] As a member of the epidermal growth factor (EGF) family, TGF-α is a mitogenic polypeptide.[6] The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling.

TGFA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1GK5, 1MOX, 1YUF, 1YUG, 2TGF, 3E50, 3TGF, 4TGF
Identifiers
Aliases	TGFA, TFGA, transforming growth factor alpha, Transforming growth factor - α
External IDs	OMIM: 190170 MGI: 98724 HomoloGene: 2431 GeneCards: TGFA
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2p13.3 Start 70,447,284 bp[1] End 70,554,193 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6 D1|6 37.62 cM Start 86,195,223 bp[2] End 86,275,719 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • epidermal growth factor receptor binding • GO:0001948 protein binding • growth factor activity Cellular component • integral component of membrane • endoplasmic reticulum membrane • membrane • cell membrane • integral component of plasma membrane • extracellular region • cell surface • basolateral plasma membrane • perinuclear region of cytoplasm • ER to Golgi transport vesicle membrane • endoplasmic reticulum-Golgi intermediate compartment membrane • cytoplasmic vesicle • Golgi membrane • extracellular • clathrin-coated vesicle membrane Biological process • positive regulation of epidermal growth factor-activated receptor activity • positive regulation of epithelial cell proliferation • ER to Golgi vesicle-mediated transport • COPII vesicle coating • activation of MAPK activity • cellular proliferation • positive regulation of mitotic nuclear division • positive regulation of cell division • regulation of transcription from RNA polymerase II promoter • epidermal growth factor receptor signaling pathway • positive regulation of cell proliferation • MAPK cascade • signal transduction • negative regulation of epidermal growth factor receptor signaling pathway • positive regulation of protein kinase B signaling • membrane organization • GO:0007243 intracellular signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	7039	21802
Ensembl	ENSG00000163235	ENSMUSG00000029999
UniProt	P01135	P48030
RefSeq (mRNA)	NM_001099691 NM_001308158 NM_001308159 NM_003236	NM_031199
RefSeq (protein)	NP_001093161 NP_001295087 NP_001295088 NP_003227	NP_112476
Location (UCSC)	Chr 2: 70.45 – 70.55 Mb	Chr 6: 86.2 – 86.28 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

TGF-α is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate.[5]

Synthesis

TGF-α is synthesized internally as part of a 160 (human) or 159 (rat) amino acid transmembrane precursor.[7] The precursor is composed of an extracellular domain containing a hydrophobic transmembrane domain, 50 amino acids of TGF-α, and a 35-residue-long cytoplasmic domain.[7] In its smallest form, TGF-α has six cysteines linked together via three disulfide bridges. Collectively, all members of the EGF/TGF-α family share this structure. The protein, however, is not directly related to TGF-β.

Limited success has resulted from attempts to synthesize of a reductant molecule to TGF-α that displays a similar biological profile.[8]

Synthesis in the stomach

In the stomach, TGF-α is manufactured within the normal gastric mucosa.[9] TGF-α has been shown to inhibit gastric acid secretion.

Function

TGF-α can be produced in macrophages, brain cells, and keratinocytes. TGF-α induces epithelial development. Considering that TGF-α is a member of the EGF family, the biological actions of TGF-α and EGF are similar. For instance, TGF-α and EGF bind to the same receptor. When TGF-α binds to EGFR it can initiate multiple cell proliferation events.[8] Cell proliferation events that involve TGF-α bound to EGFR include wound healing and embryogenesis. TGF-α is also involved in tumerogenesis and believed to promote angiogenesis.[7]

TGF-α has also been shown to stimulate neural cell proliferation in the adult injured brain.[10]

Receptor

A 170-kDa glycosylated protein known as the EGF receptor binds to TGF-α allowing the polypeptide to function in various signaling pathways.[6] The EGF receptor is characterized by having an extracellular domain that has numerous amino acid motifs. EGFR is essential for a single transmembrane domain, an intracellular domain (containing tyrosine kinase activity), and ligand recognition.[6] As a membrane anchored-growth factor, TGF-α can be cleaved from an integral membrane glycoprotein via a protease.[7] Soluble forms of TGF-α resulting from the cleavage have the capacity to activate EGFR. EGFR can be activated from a membrane-anchored growth factor as well.

When TGF-α binds to EGFR it dimerizes triggering phosphorylation of a protein-tyrosine kinase. The activity of protein-tyrosine kinase causes an autophosphorylation to occur among several tyrosine residues within EGFR, influencing activation and signaling of other proteins that interact in many signal transduction pathways.

Epidermal growth factor receptor (EGFR) signaling pathway upon binding to TGF-α.

Animal studies

In an animal model of Parkinson's disease where dopaminergic neurons have been damaged by 6-hydroxydopamine, infusion of TGF-α into the brain caused an increase in the number of neuronal precursor cells.[10] However TGF-α treatment did not result in neurogenesis dopaminergic neurons.[11]

Human studies

Neuroendocrine system

The EGF/TGF-α family has been shown to regulate luteinizing hormone-releasing hormone (LHRH) through a glial-neuronal interactive process.[6] Produced in hypothalamic astrocytes, TGF-α indirectly stimulates LHRH release through various intermediates. As a result, TGF-α is a physiological component essential to the initiation process of female puberty.[6]

Suprachiasmatic nucleus

TGF-α has also been observed to be highly expressed in the suprachiasmatic nucleus (SCN) (5). This finding suggests a role for EGFR signaling in the regulation of CLOCK and circadian rhythms within the SCN.[12] Similar studies have shown that when injected into the third ventricle TGF-α can suppress circadian locomotor behavior along with drinking or eating activities.[12]

Tumors

This protein shows potential use as a prognostic biomarker in various tumors, like gastric carcinoma.[13] or melanoma has been suggested.[14] Elevated TGF-α is associated with Menetrier's disease, a precancerous condition of the stomach.[15]

Interactions

TGF alpha has been shown to interact with GORASP1[16] and GORASP2.[16]

See also

• Transforming growth factor

References

1. GRCh38: Ensembl release 89: ENSG00000163235 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029999 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: TGFA transforming growth factor alpha".
6. Ojeda, S. R.; Ma, Y. J.; Rage, F. (September 1997). "The transforming growth factor alpha gene family is involved in the neuroendocrine control of mammalian puberty". Molecular Psychiatry. 2 (5): 355–358. doi:10.1038/sj.mp.4000307. PMID 9322223.
7. Ferrer, I.; Alcantara, S.; Ballabriga, J.; Olive, M.; Blanco, R.; Rivera, R.; Carmona, M.; Berruezo, M.; Pitarch, S.; Planas, A. Transforming growth factor- α (TGF-α) and epidermal growth factor-receptor (EGF-R) immunoreactivity in normal and pathologic brain. Prog. Neurobiol. 1996, 49, 99.
8. McInnes, C; Wang, J; Al Moustafa, AE; Yansouni, C; O'Connor-McCourt, M; Sykes, BD (1998). "Structure-based minimization of transforming growth factor-alpha (TGF-alpha) through NMR analysis of the receptor-bound ligand. Design, solution structure, and activity of TGF-alpha 8-50"". J. Biol. Chem. 273 (42): 27357–63. doi:10.1074/jbc.273.42.27357. PMID 9765263.
9. Coffey, R.; Gangarosa, L.; Damstrup, L.; Dempsey, P. Basic actions of transforming growth factor- α and related peptides. Eur. J. Gastroen. Hepat. 1995, 7, 923.
10. Fallon J, Reid S, Kinyamu R, Opole I, Opole R, Baratta J, Korc M, Endo TL, Duong A, Nguyen G, Karkehabadhi M, Twardzik D, Patel S, Loughlin S (2000). "In vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain". Proceedings of the National Academy of Sciences of the United States of America. 97 (26): 14686–91. doi:10.1073/pnas.97.26.14686. PMC 18979. PMID 11121069.CS1 maint: uses authors parameter (link)
11. Cooper O, Isacson O (October 2004). "Intrastriatal transforming growth factor alpha delivery to a model of Parkinson's disease induces proliferation and migration of endogenous adult neural progenitor cells without differentiation into dopaminergic neurons". J. Neurosci. 24 (41): 8924–31. doi:10.1523/JNEUROSCI.2344-04.2004. PMC 2613225. PMID 15483111.
12. Hao, H.; Schwaber, J. Epidermal growth factor receptor induced Erk phosphorylation in the suprachiasmatic nucleus. Brain Res. 2006, 1088, 45.
13. Fanelli MF (Aug 2012). "The influence of transforming growth factor-α, cyclooxygenase-2, matrix metalloproteinase (MMP)-7, MMP-9 and CXCR4 proteins involved in epithelial-mesenchymal transition on overall survival of patients with gastric cancer". Histopathology. 61 (2): 153–61. doi:10.1111/j.1365-2559.2011.04139.x. PMID 22582975. S2CID 6566296.
14. Tarhini AA (Jan 2014). "A four-marker signature of TNF-RII, TGF-α, TIMP-1 and CRP is prognostic of worse survival in high-risk surgically resected melanoma". J Transl Med. 12: 19. doi:10.1186/1479-5876-12-19. PMC 3909384. PMID 24457057.
15. Coffey, Robert J.; Washington, Mary Kay; Corless, Christopher L.; Heinrich, Michael C. (2007). "Ménétrier disease and gastrointestinal stromal tumors: hyperproliferative disorders of the stomach". Journal of Clinical Investigation. 117 (1): 70–80. doi:10.1172/JCI30491. PMC 1716220. PMID 17200708. Retrieved 2016-03-25.
16. Barr FA, Preisinger C, Kopajtich R, Körner R (December 2001). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". J. Cell Biol. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891. PMID 11739402.

Further reading

• Luetteke NC, Lee DC (1991). "Transforming growth factor alpha: expression, regulation and biological action of its integral membrane precursor". Semin. Cancer Biol. 1 (4): 265–75. PMID 2103501.
• Greten FR, Wagner M, Weber CK, Zechner U, Adler G, Schmid RM (2002). "TGF alpha transgenic mice. A model of pancreatic cancer development". Pancreatology. 1 (4): 363–8. doi:10.1159/000055835. PMID 12120215. S2CID 84256727.
• Vieira AR (2006). "Association between the transforming growth factor alpha gene and nonsyndromic oral clefts: a HuGE review". Am. J. Epidemiol. 163 (9): 790–810. doi:10.1093/aje/kwj103. PMID 16495466.
• Nasim MM, Thomas DM, Alison MR, Filipe MI (1992). "Transforming growth factor alpha expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma--an immunohistochemical study". Histopathology. 20 (4): 339–43. doi:10.1111/j.1365-2559.1992.tb00991.x. PMID 1577411. S2CID 73067240.
• Thomas DM, Nasim MM, Gullick WJ, Alison MR (1992). "Immunoreactivity of transforming growth factor alpha in the normal adult gastrointestinal tract". Gut. 33 (5): 628–31. doi:10.1136/gut.33.5.628. PMC 1379291. PMID 1612477.
• Bean MF, Carr SA (1992). "Characterization of disulfide bond position in proteins and sequence analysis of cystine-bridged peptides by tandem mass spectrometry". Anal. Biochem. 201 (2): 216–26. doi:10.1016/0003-2697(92)90331-Z. PMID 1632509.
• Lei ZM, Rao CV (1992). "Expression of epidermal growth factor (EGF) receptor and its ligands, EGF and transforming growth factor-alpha, in human fallopian tubes". Endocrinology. 131 (2): 947–57. doi:10.1210/en.131.2.947. PMID 1639032.
• Werner S, Roth WK, Bates B, Goldfarb M, Hofschneider PH (1991). "Fibroblast growth factor 5 proto-oncogene is expressed in normal human fibroblasts and induced by serum growth factors". Oncogene. 6 (11): 2137–44. PMID 1658709.
• Saeki T, Cristiano A, Lynch MJ, Brattain M, Kim N, Normanno N, Kenney N, Ciardiello F, Salomon DS (1992). "Regulation by estrogen through the 5'-flanking region of the transforming growth factor alpha gene". Mol. Endocrinol. 5 (12): 1955–63. doi:10.1210/mend-5-12-1955. PMID 1791840.
• Harvey TS, Wilkinson AJ, Tappin MJ, Cooke RM, Campbell ID (1991). "The solution structure of human transforming growth factor alpha". Eur. J. Biochem. 198 (3): 555–62. doi:10.1111/j.1432-1033.1991.tb16050.x. PMID 2050136.
• Kline TP, Brown FK, Brown SC, Jeffs PW, Kopple KD, Mueller L (1991). "Solution structures of human transforming growth factor alpha derived from 1H NMR data". Biochemistry. 29 (34): 7805–13. doi:10.1021/bi00486a005. PMID 2261437.
• Jakowlew SB, Kondaiah P, Dillard PJ, Sporn MB, Roberts AB (1989). "A novel low molecular weight ribonucleic acid (RNA) related to transforming growth factor alpha messenger RNA". Mol. Endocrinol. 2 (11): 1056–63. doi:10.1210/mend-2-11-1056. PMID 2464748.
• Jakobovits EB, Schlokat U, Vannice JL, Derynck R, Levinson AD (1989). "The human transforming growth factor alpha promoter directs transcription initiation from a single site in the absence of a TATA sequence". Mol. Cell. Biol. 8 (12): 5549–54. doi:10.1128/mcb.8.12.5549. PMC 365660. PMID 2907605.
• Tricoli JV, Nakai H, Byers MG, Rall LB, Bell GI, Shows TB (1986). "The gene for human transforming growth factor alpha is on the short arm of chromosome 2". Cytogenet. Cell Genet. 42 (1–2): 94–8. doi:10.1159/000132258. PMID 3459638.
• Lee DC, Rose TM, Webb NR, Todaro GJ (1985). "Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha". Nature. 313 (6002): 489–91. doi:10.1038/313489a0. PMID 3855503. S2CID 4358296.
• Derynck R, Roberts AB, Winkler ME, Chen EY, Goeddel DV (1984). "Human transforming growth factor-alpha: precursor structure and expression in E. coli". Cell. 38 (1): 287–97. doi:10.1016/0092-8674(84)90550-6. PMID 6088071. S2CID 53275849.
• Ogbureke KU, MacDaniel RK, Jacob RS, Durban EM (1995). "Distribution of immunoreactive transforming growth factor-alpha in non-neoplastic human salivary glands". Histol. Histopathol. 10 (3): 691–6. PMID 7579819.
• Walz TM, Malm C, Nishikawa BK, Wasteson A (1995). "Transforming growth factor-alpha (TGF-alpha) in human bone marrow: demonstration of TGF-alpha in erythroblasts and eosinophilic precursor cells and of epidermal growth factor receptors in blastlike cells of myelomonocytic origin". Blood. 85 (9): 2385–92. doi:10.1182/blood.V85.9.2385.bloodjournal8592385. PMID 7727772.
• Patel B, Hiscott P, Charteris D, Mather J, McLeod D, Boulton M (1994). "Retinal and preretinal localisation of epidermal growth factor, transforming growth factor alpha, and their receptor in proliferative diabetic retinopathy". The British Journal of Ophthalmology. 78 (9): 714–8. doi:10.1136/bjo.78.9.714. PMC 504912. PMID 7947554.

External links

• Transforming+Growth+Factor+alpha at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.