Prostacyclin receptor

The Prostacyclin receptor , also termed the prostaglandin I2 receptor or just IP, is a receptor belonging to the prostaglandin (PG) group of receptors. IP binds to and mediates the biological actions of prostacyclin (also termed Prostaglandin I₂, PGI₂, or when used as a drug, epoprostenol). IP is encoded in humans by the PTGIR gene. While possessing many functions as defined in animal model studies, the major clinical relevancy of IP is as a powerful vasodilator: stimulators of IP are used to treat severe and even life-threatening diseases involving pathological vasoconstriction.

PTGIR
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4F8K
Identifiers
Aliases	PTGIR, IP, PRIPR, prostaglandin I2 (prostacyclin) receptor (IP), prostaglandin I2 receptor
External IDs	OMIM: 600022 MGI: 99535 HomoloGene: 7496 GeneCards: PTGIR
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q13.32 Start 46,620,468 bp[1] End 46,625,089 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 A2|7 9.15 cM Start 16,906,490 bp[2] End 16,910,905 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • G-protein coupled receptor activity • signal transducer activity • guanyl-nucleotide exchange factor activity • prostacyclin receptor activity Cellular component • integral component of membrane • cytosol • cell membrane • integral component of plasma membrane • membrane Biological process • negative regulation of platelet-derived growth factor receptor signaling pathway • cell-cell signaling • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger • response to lipopolysaccharide • negative regulation of smooth muscle cell proliferation • adenylate cyclase-activating G-protein coupled receptor signaling pathway • signal transduction • G-protein coupled receptor signaling pathway • inflammatory response • positive regulation of cytosolic calcium ion concentration Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5739	19222
Ensembl	ENSG00000160013	ENSMUSG00000043017
UniProt	P43119	P43252
RefSeq (mRNA)	NM_000960	NM_008967
RefSeq (protein)	NP_000951	NP_032993
Location (UCSC)	Chr 19: 46.62 – 46.63 Mb	Chr 7: 16.91 – 16.91 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Gene

The PTGIR gene is located on human chromosome 19 at position q13.32 (i.e. 19q13.32), contains 6 exons, and codes for a G protein coupled receptor (GPCR) of the rhodopsin-like receptor family, Subfamily A14 (see rhodopsin-like receptors#Subfamily A14).[5]

Expression

IP is most highly expressed in brain and thymus and is readily detected in most other tissues. It is found throughout the vascular network on endothelium and smooth muscle cells.[5][6]

Ligands

Activating ligands

Standard prostanoids have the following relative efficacies as receptor ligands in binding to and activating IP: PGI₂>>PGD2=PGE2=PGF2α>TXA2. In typical binding studies, PGI₂ has one-half of its maximal binding capacity and cell-stimulating actions at ~1 nanomolar whereas the other prostaglandins are >50-fold to 100-fold weaker than this. However, PGI₂ is very unstable, spontaneously converting to a far less active derivative 6-keto-PGF1 alpha within 1 minute of its formation. This instability makes defining the exact affinity of PGI₂ for IP difficult. It also makes it important to have stable synthetic analogs of PGI₂ for clinical usage. The most potent of these receptor agonists for binding to and activating IP are iloprost, taprostene, and esuberaprost which have K_d values (i.e. concentrations which bind to half of available IP receptors) in the low nanamole/liter range (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).[7]

Inhibiting ligands

Several synthetic compounds bind to, but do not activate, IP and thereby inhibit its activation by the activating ligands just described. These receptor antagonists include RO1138452, RO3244794, TG6-129, and BAY-73-1449, all of which have K_d values for IP at or beneath low nanomol/liter levels (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).

Mechanism of cell activation

IP is classified as a relaxant type of prostenoid receptor based on its ability, upon activation, to relax certain pre-contracted smooth muscle preparations and smooth muscle-containing tissues such as those of pulmonary arteries and veins.[8] When bound to PGI₂ or other of its agonists, IP stimulates one or more of three types of G protein complexes, depending on cell type: a) Gs alpha subunit-Gβγ complexes which release Gs that then stimulates adenyl cyclase to raise intracellular levels of cAMP and thereby activate cAMP-regulated protein kinases A-dependent cell signaling pathways (see PKA); b) Gq alpha subunit-Gβγ complexes which release Gq that then stimulates other cell signaling pathways (e.g. phospholipase C/IP3/cell Ca²⁺ mobilization/diacylglycerol/protein kinase Cs, calmodulin-modulated myosin light chain kinase, RAF/MEK/Mitogen-activated protein kinases, PKC/Ca²⁺/Calcineurin/Nuclear factor of activated T-cells; and EGF cellular receptors; and c) Gi alpha subunit-Giβγ) complexes which releases Gi that then simulates phospholipase C to cleave phosphatidylinositol triphosphate into inositol triphosphate that raises intracellular CaCa² levels thereby regulating Calcium signaling pathways and diacylglycerol that activates certain protein kinase C enzymes )that phosphorylate and thereby regulate target proteins involved in cell signaling (see Protein kinase C#Function). Studies suggest that stimulation of Gsβγ complexes is required for activation of the Gqβγ- and Giβγ-dependent pathways.[7][9][10][11] In certain cells, activation of IP also stimulates G¹²/G¹³-Gβγ G proteins to activate the Rho family of GTPases signaling proteins and Gi-Gβγ G proteins to activateRaf/MEK/mitogen-activated kinase pathways.

Functions

Studies using animals genetically engineered to lack IP and examining the actions of EP4 receptor agonists in animals as well as animal and human tissues indicate that this receptor serves various functions. It has been regarded as the most successful therapeutic target among the 9 prostanoid receptors.[10]

Platelets

IP gene knockout mice (i.e. IP(-/-) mice) exhibit increased tendency to thrombosis in response to experimentally-induced Endothelium, a result which appears to reflect, at least in part, the loss of IP's anti-platelet activity.[12][13] IP activation of animal and human platelets inhibits their aggreatation response and as one consequence of this inhibition of platelet-dependent blood clotting. The PGI₂-IP axis along with the production of nitric oxide, acting together additively and potentially synergistically, are powerful and physiological negative regulators of platelet function and thereby blood clotting in humans. Studies suggest that the PGI₂-IP axis is impaired in patients with a tendency to develop pathological thrombosis such as occurs in obesity, diabetes, and coronary artery disease.[10][14]

Cardiovascular system

IP activation stimulates the dilation of arteries and veins in various animal models as well as in humans. It increases the blood flow through, for example, the pulmonary, coronary, retinal and choroid circulation. Inhaled PGI₂ causes a modest fall in diastolic and small fall in systolic blood pressure in humans. This action involves IP's ability to relax vascular smooth muscle and is considered to be one of the fundamental functions of IP receptors. Furthermore, IP(-/-) mice on a high salt diet develop significantly higher levels of hypertension, cardiac fibrosis, and cardiac hypertrophy than control mice. The vasodilating and, perhaps, platelet-inhibiting effects of IP receptors likely underlie its ability suppress hypertension and protect tissues such as the heart in this model as well as the heart, brain, and gastrointestinal tract in various animal models of ischemic injury.[10] Indeed, IP agonists are used to treat patients pathological vasoconstriction diseases.[15] The injection of IP activators into the skin of rodents increases local capillary permeability and swelling; IP(-/-) mice fail to show this increased capillary permeability and swelling in response not only to IP activators but also in a model of carrageenan- or bradykinin-induced paw edema. IP antagonists likewise reduce experimentally-induced capillary permeability and swelling in rats. This actions is also considered a physiological function of IP receptors,[7][10] but can contribute to the toxicity of IP activators in patients by inducing, for example, life-threatening pulmonary edema.[15]

IP activators inhibit the adherence of circulating platelets and leukocytes adherence to vascular endothelium thereby blocking their entry into sites of tissue disturbance. The activators also inhibit vascular smooth muscle cells from proliferation by blocking these cells' growth cycle and triggering their apoptosis (i.e. cell death). These actions, along with its anti-inflammatory effects, may underlie the ability of IP gene knockout in an ApoE(−/−) mouse model to cause an accelerated rate of developing atherosclerosis.[7] [10]

Inflammation

Mouse studies indicate that the PGI₂-IP axis activates cellular signaling pathways that tend to suppress allergic inflammation. The axis inhibits bone marrow-derived dendritic cells (i.e. antigen-presenting cells that process antigen material, present it on their surfaces for delivery to T cells, and otherwise regulate innate and adaptive immune system responses) from producing pro-inflammatory cytokines (e.g. IL-12, TNF-alpha, IL-1-alpha, and IL-6) while stimulating them to increase production of the anti-inflammatory cytokine, IL-10. IP receptor activation of these cells also blocks their lipopolysaccharide-stimulated expression of pro-inflammatory cell surface proteins (i.e. CD86, CD40, and MHC class II molecules) that are critical for developing adaptive immune responses. IL receptor-activated bone marrow-derived dendritic cells showed a greatly reduced ability to stimulate the proliferation of T helper cell as well as the ability of these cells to produce pro-allergic cytokines (i.e. IL-5 and IL-13)s. In a mouse model of allergic inflammation, PGI₂ reduced the maturation and migration of lung mature dendritic cells to Mediastinal lymph nodes while increasing the egress of immature dendritic cells away from the lung. These effects resulted in a decrease in allergen-induced responses of the cells mediating allergic reactivity, TH-2 cells. These IP-induced responses likely contribute to its apparent function in inhibiting certain mouse inflammation responses as exemplified by the failure of IP receptor deficient mice to develop full lung airway allergic responses to ovalbumin in a model of allergic inflammation.[7][6]

In human studies, PGI₂ failed to alter bronchoconstriction responses to allergen but did protect against exercise-induced and ultrasonic water-induced bronchoconstriction in asthmatic patients. It also caused bronchodilation in two asthmatic patients. However, these studies were done before the availability of potent and selective IP agonists. These agonists might produce more effective inhibitor results on airways allergic diseases but their toxicity (e.g. pulmonary edema, hypotension) has tended to restrict there study in asthmatic patients.[6]

IP receptors also appear involved in suppressing non-allergic inflammatory responses. IP receptor-deficient mice exhibit a reduction in the extent and progression of inflammation in a model of collagen-induced arthritis. This effect may result from regulating the expression of arthritis-related, pro-inflammatory genes (i.e. those for IL-6, VEGF-A, and RANKL).[8][10] On the other hand, IP receptors may serve to promote non-allergic inflammatory responses: IP receptor-deficient mice exhibited increased lung inflammation in a model of bleomycin-induced pulmonary fibrosis while mice made to over-express the PGI₂-forming enzyme, Prostacyclin synthase, in their airway epithelial cells were protected against lung injury in this model.[6]

Pain perception

IP(-/-) mice exhibit little or no writhing responses in an acetic acid-induced pain model. The mouse IP receptor also appears to be involved in the development of heat-induced hyperalgesia. These and further studies using IP receptor antagonists in rats indicate that IP receptors on pain-perceiving sensory neurons of the dorsal root ganglia as well as on certain neurons in the spinal cord transmit signals for pain, particularly pain triggered by inflammation.[7][10]

Clinical significance

Toxicity

IP receptor agonists, particularly when used intravenously, have been associated with the rapid development of pulmonary edema, hypotension, bleeding due to inhibition of platelet aggregation, and tachycardia.[16][17] Clinical use of these agonists is contraindicated in patients suffering many conditions. For example, the IP agonist iloprost is contraindicated in patients with unstable angina; decompensated cardiac failure (unless under close medical supervision); severe cardiac arrhythmias; congenital or acquired heart valve defects; increased risk of bleeding; a history of myocardial infarction in the past 6 months; or a history of cerebrovascular events (e.g. stroke) within 3 months.

Vasoconstriction

IP receptor agonists are front-line drugs to treat pulmonary hypertension. Major drugs in this category include PGI₂ itself (i.e. epoprostenol), iloprost, treprostinil, and beraprost with epoprostenol being favored in some studies.[16][18][19] However, newly developed IP agonists with favorable pharmacological features such as Selexipag have been granted by the US FDA Orphan Drug status for the treatment of pulmonary hypertension. IP agonists are also to treat severe vasoconstriction in Raynaud's disease, Raynaud's disease-like syndromes, and scleroderma.[20][21] Epoprostenol causes improvements in hemodynamic parameters and oxygenation in patients suffering the acute respiratory distress syndrome but due to the limited number of randomized clinical trials and lack of studies investigating mortality, its use cannot be recommended as standard of care for this disease and should be reserved for those refractory to traditional therapies.[17] A meta-analysis of 18 clinical trials on the use of prostanoids including prinicpally IP receptor agonists on patients with severe lower limb peripheral artery disease due to diverse causes found that these drugs may reduce the extent of limb tissue that needed to be amputated. However, the studies did not support extensive use of prostanoids in patients with critical limb ischemia as an adjunct to revascularization or as an alternative to major amputation in cases which cannot undergo revascularization.[22]

Thrombotic diseases

IP receptor agonists have been used to treat Thromboangiitis obliterans, a disease involving blood clotting and inflammation of the small and medium-sized arteries and veins in the hands and feet.[23]

Genomic studies

An adenine (A) to cytosine (C) synonymous substitution at base 984 (i.e. A984C) in exon 3 of PTGIR' is the most frequent single nucleotide polymorphism (SNP) variant in a sampling of Japanese. This variant was associated with an increase in platelet activation responses in vitro and an increase in incidence of cerebral ischemia. Two other synonymous SNP variants, V53V and S328S, in PTGIR in an Italian population study were associated with enhanced platelet activation response and deep vein thrombosis.[24] The rare SNP variant 795C of 794T in the PTGIR gene is associated with an increased incidence of Aspirin-induced asthma and a greater percentage fall in the forced expiratory volume response of airways to inhalation of an aspirin like compound (lysine-acetyl salicylic acid) in a Korean population sample.[25][26]

See also

• PTGIR gene (https://www.wikigenes.org/e/gene/e/5739.html)
• PGI₂
• Prostaglandin receptors
• Eicosanoid receptor

References

1. GRCh38: Ensembl release 89: ENSG00000160013 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000043017 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. https://www.ncbi.nlm.nih.gov/gene/5739
6. Claar D, Hartert TV, Peebles RS (February 2015). "The role of prostaglandins in allergic lung inflammation and asthma". Expert Review of Respiratory Medicine. 9 (1): 55–72. doi:10.1586/17476348.2015.992783. PMC 4380345. PMID 25541289.
7. Ricciotti E, FitzGerald GA (May 2011). "Prostaglandins and inflammation". Arteriosclerosis, Thrombosis, and Vascular Biology. 31 (5): 986–1000. doi:10.1161/ATVBAHA.110.207449. PMC 3081099. PMID 21508345.
8. Matsuoka T, Narumiya S (August 2008). "The roles of prostanoids in infection and sickness behaviors". Journal of Infection and Chemotherapy. 14 (4): 270–8. doi:10.1007/s10156-008-0622-3. PMID 18709530. S2CID 207058745.
9. Oguma T, Asano K, Ishizaka A (December 2008). "Role of prostaglandin D(2) and its receptors in the pathophysiology of asthma". Allergology International. 57 (4): 307–12. doi:10.2332/allergolint.08-RAI-0033. PMID 18946232.
10. Woodward DF, Jones RL, Narumiya S (September 2011). "International Union of Basic and Clinical Pharmacology. LXXXIII: classification of prostanoid receptors, updating 15 years of progress". Pharmacological Reviews. 63 (3): 471–538. doi:10.1124/pr.110.003517. PMID 21752876.
11. Moreno JJ (February 2017). "Eicosanoid receptors: Targets for the treatment of disrupted intestinal epithelial homeostasis". European Journal of Pharmacology. 796: 7–19. doi:10.1016/j.ejphar.2016.12.004. PMID 27940058. S2CID 1513449.
12. Stitham J, Hwa J (2016). "Prostacyclin, Atherothrombosis and Diabetes Mellitus: Physiologic and Clinical Considerations". Current Molecular Medicine. 16 (4): 328–42. doi:10.2174/1566524016666160316150728. PMID 26980701.
13. Narumiya S, Sugimoto Y, Ushikubi F (October 1999). "Prostanoid receptors: structures, properties, and functions". Physiological Reviews. 79 (4): 1193–226. doi:10.1152/physrev.1999.79.4.1193. PMID 10508233.
14. Procter NE, Hurst NL, Nooney VB, Imam H, De Caterina R, Chirkov YY, Horowitz JD (October 2016). "New Developments in Platelet Cyclic Nucleotide Signalling: Therapeutic Implications". Cardiovascular Drugs and Therapy. 30 (5): 505–513. doi:10.1007/s10557-016-6671-4. PMID 27358171. S2CID 26734051.
15. Benyahia C, Boukais K, Gomez I, Silverstein A, Clapp L, Fabre A, Danel C, Leséche G, Longrois D, Norel X (December 2013). "A comparative study of PGI2 mimetics used clinically on the vasorelaxation of human pulmonary arteries and veins, role of the DP-receptor". Prostaglandins & Other Lipid Mediators. 107: 48–55. doi:10.1016/j.prostaglandins.2013.07.001. PMID 23850788.
16. McGinn K, Reichert M (January 2016). "A Comparison of Inhaled Nitric Oxide Versus Inhaled Epoprostenol for Acute Pulmonary Hypertension Following Cardiac Surgery". The Annals of Pharmacotherapy. 50 (1): 22–6. doi:10.1177/1060028015608865. PMID 26438636. S2CID 20499189.
17. Searcy RJ, Morales JR, Ferreira JA, Johnson DW (December 2015). "The role of inhaled prostacyclin in treating acute respiratory distress syndrome". Therapeutic Advances in Respiratory Disease. 9 (6): 302–12. doi:10.1177/1753465815599345. PMID 26294418. S2CID 19698203.
18. Zhang H, Li X, Huang J, Li H, Su Z, Wang J (January 2016). "Comparative Efficacy and Safety of Prostacyclin Analogs for Pulmonary Arterial Hypertension: A Network Meta-Analysis". Medicine. 95 (4): e2575. doi:10.1097/MD.0000000000002575. PMC 5291571. PMID 26825901.
19. Sitbon O, Vonk Noordegraaf A (January 2017). "Epoprostenol and pulmonary arterial hypertension: 20 years of clinical experience". European Respiratory Review. 26 (143): 160055. doi:10.1183/16000617.0055-2016. PMID 28096285.
20. Poredos P, Poredos P (April 2016). "Raynaud's Syndrome: a neglected disease". International Angiology. 35 (2): 117–21. PMID 25673314.
21. Young A, Namas R, Dodge C, Khanna D (September 2016). "Hand Impairment in Systemic Sclerosis: Various Manifestations and Currently Available Treatment". Current Treatment Options in Rheumatology. 2 (3): 252–269. doi:10.1007/s40674-016-0052-9. PMC 5176259. PMID 28018840.
22. Vitale V, Monami M, Mannucci E (2016). "Prostanoids in patients with peripheral arterial disease: A meta-analysis of placebo-controlled randomized clinical trials". Journal of Diabetes and Its Complications. 30 (1): 161–6. doi:10.1016/j.jdiacomp.2015.09.006. PMID 26516035.
23. Cacione, Daniel G.; Macedo, Cristiane R.; do Carmo Novaes, Frederico; Baptista-Silva, Jose Cc (4 May 2020). "Pharmacological treatment for Buerger's disease". The Cochrane Database of Systematic Reviews. 5: CD011033. doi:10.1002/14651858.CD011033.pub4. ISSN 1469-493X. PMC 7197514. PMID 32364620.
24. Cornejo-García JA, Perkins JR, Jurado-Escobar R, García-Martín E, Agúndez JA, Viguera E, Pérez-Sánchez N, Blanca-López N (2016). "Pharmacogenomics of Prostaglandin and Leukotriene Receptors". Frontiers in Pharmacology. 7: 316. doi:10.3389/fphar.2016.00316. PMC 5030812. PMID 27708579.
25. Kim SH, Choi JH, Park HS, Holloway JW, Lee SK, Park CS, Shin HD (May 2005). "Association of thromboxane A2 receptor gene polymorphism with the phenotype of acetyl salicylic acid-intolerant asthma". Clinical and Experimental Allergy. 35 (5): 585–90. doi:10.1111/j.1365-2222.2005.02220.x. PMID 15898979. S2CID 29436581.
26. Thompson MD, Capra V, Clunes MT, Rovati GE, Stankova J, Maj MC, Duffy DL (2016). "Cysteinyl Leukotrienes Pathway Genes, Atopic Asthma and Drug Response: From Population Isolates to Large Genome-Wide Association Studies". Frontiers in Pharmacology. 7: 299. doi:10.3389/fphar.2016.00299. PMC 5131607. PMID 27990118.

Further reading

• Coleman RA, Smith WL, Narumiya S (June 1994). "International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes". Pharmacological Reviews. 46 (2): 205–29. PMID 7938166.
• Rauvala H, Peng HB (June 1997). "HB-GAM (heparin-binding growth-associated molecule) and heparin-type glycans in the development and plasticity of neuron-target contacts". Progress in Neurobiology. 52 (2): 127–44. doi:10.1016/S0301-0082(97)00007-5. PMID 9185236. S2CID 38987199.
• Smyth EM, FitzGerald GA (2003). Human prostacyclin receptor. Vitamins & Hormones. 65. pp. 149–65. doi:10.1016/S0083-6729(02)65063-0. ISBN 978-0-12-709865-4. PMID 12481546.
• Boie Y, Rushmore TH, Darmon-Goodwin A, Grygorczyk R, Slipetz DM, Metters KM, Abramovitz M (April 1994). "Cloning and expression of a cDNA for the human prostanoid IP receptor". The Journal of Biological Chemistry. 269 (16): 12173–8. PMID 7512962.
• Katsuyama M, Sugimoto Y, Namba T, Irie A, Negishi M, Narumiya S, Ichikawa A (May 1994). "Cloning and expression of a cDNA for the human prostacyclin receptor". FEBS Letters. 344 (1): 74–8. doi:10.1016/0014-5793(94)00355-6. PMID 7514139. S2CID 12203377.
• Ogawa Y, Tanaka I, Inoue M, Yoshitake Y, Isse N, Nakagawa O, Usui T, Itoh H, Yoshimasa T, Narumiya S (May 1995). "Structural organization and chromosomal assignment of the human prostacyclin receptor gene". Genomics. 27 (1): 142–8. doi:10.1006/geno.1995.1016. PMID 7665161.
• Duncan AM, Anderson LL, Funk CD, Abramovitz M, Adam M (February 1995). "Chromosomal localization of the human prostanoid receptor gene family". Genomics. 25 (3): 740–2. doi:10.1016/0888-7543(95)80022-E. PMID 7759114.
• Nakagawa O, Tanaka I, Usui T, Harada M, Sasaki Y, Itoh H, Yoshimasa T, Namba T, Narumiya S, Nakao K (October 1994). "Molecular cloning of human prostacyclin receptor cDNA and its gene expression in the cardiovascular system". Circulation. 90 (4): 1643–7. doi:10.1161/01.cir.90.4.1643. PMID 7923647.
• Bonaldo MF, Lennon G, Soares MB (September 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Sasaki Y, Takahashi T, Tanaka I, Nakamura K, Okuno Y, Nakagawa O, Narumiya S, Nakao K (August 1997). "Expression of prostacyclin receptor in human megakaryocytes". Blood. 90 (3): 1039–46. doi:10.1182/blood.V90.3.1039. PMID 9242534.
• Fisch A, Tobusch K, Veit K, Meyer J, Darius H (August 1997). "Prostacyclin receptor desensitization is a reversible phenomenon in human platelets". Circulation. 96 (3): 756–60. doi:10.1161/01.cir.96.3.756. PMID 9264479.
• Smyth EM, Li WH, FitzGerald GA (September 1998). "Phosphorylation of the prostacyclin receptor during homologous desensitization. A critical role for protein kinase c". The Journal of Biological Chemistry. 273 (36): 23258–66. doi:10.1074/jbc.273.36.23258. PMID 9722557.
• Kömhoff M, Lesener B, Nakao K, Seyberth HW, Nüsing RM (December 1998). "Localization of the prostacyclin receptor in human kidney". Kidney International. 54 (6): 1899–908. doi:10.1046/j.1523-1755.1998.00213.x. PMID 9853255.
• Hayes JS, Lawler OA, Walsh MT, Kinsella BT (August 1999). "The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling". The Journal of Biological Chemistry. 274 (34): 23707–18. doi:10.1074/jbc.274.34.23707. PMID 10446129.
• Smyth EM, Austin SC, Reilly MP, FitzGerald GA (October 2000). "Internalization and sequestration of the human prostacyclin receptor". The Journal of Biological Chemistry. 275 (41): 32037–45. doi:10.1074/jbc.M003873200. PMID 10889200.
• Lawler OA, Miggin SM, Kinsella BT (September 2001). "Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling". The Journal of Biological Chemistry. 276 (36): 33596–607. doi:10.1074/jbc.M104434200. PMID 11443126.
• Zhang Z, Austin SC, Smyth EM (September 2001). "Glycosylation of the human prostacyclin receptor: role in ligand binding and signal transduction". Molecular Pharmacology. 60 (3): 480–7. PMID 11502878.
• Fortier I, Patry C, Lora M, Samadfan R, de Brum-Fernandes AJ (August 2001). "Immunohistochemical localization of the prostacyclin receptor (IP) human bone". Prostaglandins, Leukotrienes, and Essential Fatty Acids. 65 (2): 79–83. doi:10.1054/plef.2001.0292. PMID 11545623.

External links

• "Prostanoid Receptors: IP₁". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical Pharmacology.
• Overview of all the structural information available in the PDB for UniProt: P43252 (Mouse Prostacyclin receptor) at the PDBe-KB.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.